PMID- 32693640 OWN - NLM STAT- MEDLINE DCOM- 20210719 LR - 20210719 IS - 1555-3892 (Electronic) IS - 0963-6897 (Print) IS - 0963-6897 (Linking) VI - 29 DP - 2020 Jan-Dec TI - NEAT1 Negatively Regulates Cell Proliferation and Migration of Neuroblastoma Cells by miR-183-5p/FOXP1 Via the ERK/AKT Pathway. PG - 963689720943608 LID - 10.1177/0963689720943608 [doi] LID - 0963689720943608 AB - Neuroblastoma, a malignant tumor of the sympathetic nervous system, is an aggressive extracranial tumor in childhood. Long noncoding RNAs (lncRNAs) have been discovered to play a key role in the eukaryotic regulatory gene network and be involved in a wide variety of biological processes. We observed that the expression of lncRNA nuclear-enriched abundant transcript-1 (NEAT1) was significantly decreased in human neuroblastoma tissues and cell lines, compared with the normal. We observed cell proliferation, migration, and invasion with Cell Counting Kit-8 assay, colony formation assay, and Transwell assay to investigate the effects of NEAT1, miR-183-5p, or FOXP1 on neuroblastoma cells. And we also used StarBase and luciferase reporter gene assay to predict and confirm the interaction of NEAT1, miR-183-5p, and FOXP1 in neuroblastoma cells. First, overexpression of NEAT1 suppressed cell proliferation and played a key role in cell migration and invasion. In addition, NEAT1 was demonstrated to directly interact with miR-183-5p and exerted its antioncogenic role in neuroblastoma by negatively regulating miR-183-5p expression. miR-183-5p suppressed the expression of FOXP1 and regulated cell proliferation and migration by directly targeting FOXP1 mRNA 3'-untranslated region. Moreover, FOXP1 antagonized the effect of miR-183-5p on the phosphorylation of extracellular-regulated kinase/protein kinase B (ERK/AKT), while FOXP1 siRNA increased the reduced phosphorylation of ERK/AKT caused by miR-183-5p inhibitor in neuroblastoma cells. Taken together, these data showed that NEAT1 negatively regulated cell proliferation and migration of neuroblastoma by the miR-183-5p/FOXP1 axis via suppression of the ERK/AKT pathway. Our findings may provide a new target for the study of pathogenesis and treatment of neuroblastoma. FAU - Pan, Weikang AU - Pan W AD - Pediatric Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. AD - Both the authors contributed equally to this article. FAU - Wu, Ali AU - Wu A AD - Department of Endoscopy, Shaanxi Nuclear Industry, Xianyang, China. AD - Both the authors contributed equally to this article. FAU - Yu, Hui AU - Yu H AD - Pediatric Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. FAU - Yu, Qiang AU - Yu Q AD - Pediatric Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. FAU - Zheng, Baijun AU - Zheng B AD - Pediatric Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. FAU - Yang, Weili AU - Yang W AD - Pediatric Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. FAU - Tian, Donghao AU - Tian D AD - Pediatric Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. FAU - Gao, Ya AU - Gao Y AD - Pediatric Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. FAU - Li, Peng AU - Li P AUID- ORCID: 0000-0002-2368-8659 AD - Pediatric Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Cell Transplant JT - Cell transplantation JID - 9208854 RN - 0 (FOXP1 protein, human) RN - 0 (Forkhead Transcription Factors) RN - 0 (MIRN183 microRNA, human) RN - 0 (MicroRNAs) RN - 0 (NEAT1 long non-coding RNA, human) RN - 0 (RNA, Long Noncoding) RN - 0 (Repressor Proteins) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) SB - IM MH - Cell Movement MH - Cell Proliferation MH - Forkhead Transcription Factors/*metabolism MH - Humans MH - MAP Kinase Signaling System/*physiology MH - MicroRNAs/*metabolism MH - Neuroblastoma/*genetics MH - Proto-Oncogene Proteins c-akt/*metabolism MH - RNA, Long Noncoding/*metabolism MH - Repressor Proteins/*metabolism MH - Transfection PMC - PMC7563027 OTO - NOTNLM OT - FOXP1 OT - NEAT1 OT - miR-183-5p OT - neuroblastoma OT - the ERK/AKT pathway COIS- Declaration of Conflicting Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2020/07/23 06:00 MHDA- 2021/07/20 06:00 PMCR- 2020/07/22 CRDT- 2020/07/23 06:00 PHST- 2020/07/23 06:00 [entrez] PHST- 2020/07/23 06:00 [pubmed] PHST- 2021/07/20 06:00 [medline] PHST- 2020/07/22 00:00 [pmc-release] AID - 10.1177_0963689720943608 [pii] AID - 10.1177/0963689720943608 [doi] PST - ppublish SO - Cell Transplant. 2020 Jan-Dec;29:963689720943608. doi: 10.1177/0963689720943608.