PMID- 32694391
OWN - NLM
STAT- MEDLINE
DCOM- 20211215
LR  - 20211215
IS  - 1540-0514 (Electronic)
IS  - 1073-2322 (Linking)
VI  - 55
IP  - 1
DP  - 2021 Jan 1
TI  - Prevention of Severe Intestinal Barrier Dysfunction Through a Single-Species 
      Probiotics is Associated With the Activation of Microbiome-Mediated 
      Glutamate-Glutamine Biosynthesis.
PG  - 128-137
LID - 10.1097/SHK.0000000000001593 [doi]
AB  - INTRODUCTION: Intra-abdominal hypertension (IAH), the leading complication in the 
      intensive care unit, significantly disturbs the gut microbial composition by 
      decreasing the relative abundance of Lactobacillus and increasing the relative 
      abundance of opportunistic infectious bacteria. METHODS: To evaluate the 
      preventative effect of Lactobacillus-based probiotics on IAH-induced intestinal 
      barrier damages, a single-species probiotics (L92) and a multispecies probiotics 
      (VSL#3) were introduced orally to Sprague-Dawley rats for 7 days before inducing 
      IAH. The intestinal histology and permeability to macromolecules (fluoresceine 
      isothiocyanate, FITC-dextran, N = 8 for each group), the parameters of 
      immunomodulatory and oxidative responses [monocyte chemotactic protein 1 (MCP-1), 
      interleukin-1beta (IL-1beta), interleukin-4 (IL-4), interleukin-10 (IL-10), 
      malonaldehyde, glutathione peroxidase (GSH- Px), catalase (CAT), and superoxide 
      dismutase; N = 4 for each group], and the microbiome profiling (N = 4 for each 
      group) were analyzed. RESULTS: Seven-day pretreatments of L92 significantly 
      alleviated the IAH-induced increase in intestinal permeability to FITC-dextran 
      and histological damage (P  < 0.0001), accompanied with the suppression of 
      inflammatory and oxidative activation. The increase of MCP-1 and IL-1beta was 
      significantly inhibited (P  < 0.05); the anti-inflammatory cytokines, IL-4, and 
      IL-10 were maintained at high levels; and the suppression of CAT (P  <  0.05) was 
      significantly reversed when pretreated with L92. On the contrary, no significant 
      protective effects were observed in the VSL#3-pretreated group. Among the 84 
      identified species, 260 MetaCyc pathways, and 217 Kyoto Encyclopedia of Genes and 
      Genomes (KEGG) pathways, the protective effects of L92 were correlated with an 
      increased relative abundance of Bacteroides finegoldii, Odoribacter splanchnicus, 
      and the global activation of amino acid biosynthesis pathways, especially the 
      glutamate-glutamine biosynthesis pathway. CONCLUSIONS: Seven-day pretreatment 
      with a single-species probiotics can prevent IAH-induced severe intestinal 
      barrier dysfunction, potentially through microbial modulation.
CI  - Copyright (c) 2020 by the Shock Society.
FAU - Leng, Yuxin
AU  - Leng Y
AD  - Department of Intensive Care Unit, Peking University Third Hospital, Beijing, 
      China.
AD  - Department of Genetics, Stanford University School of Medicine, Stanford, 
      California.
FAU - Jiang, Chao
AU  - Jiang C
AD  - Department of Genetics, Stanford University School of Medicine, Stanford, 
      California.
AD  - Life Sciences Institute, Zhejiang University, Hangzhou, China.
FAU - Xing, Xiaofang
AU  - Xing X
AD  - Department of Molecular Diagnostics, Peking University Cancer Hospital, Beijing, 
      China.
FAU - Tsai, Ming-Shian
AU  - Tsai MS
AD  - Department of Genetics, Stanford University School of Medicine, Stanford, 
      California.
FAU - Snyder, Michael
AU  - Snyder M
AD  - Department of Genetics, Stanford University School of Medicine, Stanford, 
      California.
FAU - Zhai, Aixia
AU  - Zhai A
AD  - Department of Microbiology, Harbin Medical University, Harbin, China.
FAU - Yao, Gaiqi
AU  - Yao G
AD  - Department of Intensive Care Unit, Peking University Third Hospital, Beijing, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Shock
JT  - Shock (Augusta, Ga.)
JID - 9421564
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Gastrointestinal Microbiome
MH  - Intestinal Absorption/physiology
MH  - Intestinal Diseases/metabolism/microbiology/*prevention & control
MH  - Intra-Abdominal Hypertension/*complications/metabolism
MH  - *Lactobacillus
MH  - Male
MH  - Probiotics/*therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
COIS- The authors report no conflicts of interest.
EDAT- 2020/07/23 06:00
MHDA- 2021/12/16 06:00
CRDT- 2020/07/23 06:00
PHST- 2020/07/23 06:00 [pubmed]
PHST- 2021/12/16 06:00 [medline]
PHST- 2020/07/23 06:00 [entrez]
AID - 00024382-202101000-00017 [pii]
AID - 10.1097/SHK.0000000000001593 [doi]
PST - ppublish
SO  - Shock. 2021 Jan 1;55(1):128-137. doi: 10.1097/SHK.0000000000001593.