PMID- 32695121
OWN - NLM
STAT- MEDLINE
DCOM- 20210405
LR  - 20210405
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 11
DP  - 2020
TI  - A Novel alpha/beta Hydrolase Domain Protein Derived From Haemonchus contortus Acts at 
      the Parasite-Host Interface.
PG  - 1388
LID - 10.3389/fimmu.2020.01388 [doi]
LID - 1388
AB  - The alpha/beta-hydrolase domain (ABHD) proteins belonging to alpha/beta-hydrolase (ABH) 
      superfamily are ubiquitously distributed throughout all the organisms, and their 
      functional roles have been implicated in energy metabolism, cell signaling, 
      growth and development. In our preliminary work, we identified a novel ABHD 
      protein derived from Haemonchus contortus excretory-secretory (ES) proteins 
      (HcESPs) that interacted with host T cells. Here, we demonstrated that H. 
      contortus ABHD (HcABHD) protein, expressed in all life-cycle stages of H. 
      contortus, is a mammalian ABHD17 homolog with immunodiagnostic utility and lipase 
      activity. Given its catalytic activities and immunomodulatory potentials, we 
      further investigated the functional diversity of HcABHD as an individual ES 
      protein in parasite-host interactions. HcABHD protein may serve as depalmitoylase 
      or thioesterase to suppress cell viability, inhibit cell proliferation, induce 
      intrinsic and extrinsic T cell apoptosis, and cause cell cycle arrested at G1 
      phase. Moreover, recombinant HcABHD stimuli exerted critical controls on T cell 
      cytokine production profiles, predominantly by inhibiting the secretions of 
      interleukin (IL)-4, interferon-gamma (IFN-gamma) and transforming growth factor-beta 
      (TGF-beta) 1, and promoting IL-10 production. As the immunomodulator acting at the 
      parasite-host interface, HcABHD protein may have potential applications for the 
      vaccine development of therapeutic intervention. Together, these findings may 
      help illuminate the molecular and particularly immunomodulatory aspects of ES 
      proteins and contribute to an enhanced understanding of parasite immune evasion 
      in H. contortus-host biology.
CI  - Copyright (c) 2020 Lu, Tian, Tian, Li, Yan, Xu, Song and Li.
FAU - Lu, Mingmin
AU  - Lu M
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
FAU - Tian, Xiaowei
AU  - Tian X
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
FAU - Tian, Ai-Ling
AU  - Tian AL
AD  - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of 
      Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Lanzhou, China.
FAU - Li, Charles
AU  - Li C
AD  - Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research 
      Center, Agricultural Research Service, U.S. Department of Agriculture, 
      Beltsville, MD, United States.
FAU - Yan, Ruofeng
AU  - Yan R
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
FAU - Xu, Lixin
AU  - Xu L
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
FAU - Song, Xiaokai
AU  - Song X
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
FAU - Li, Xiangrui
AU  - Li X
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200630
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Helminth Proteins)
RN  - EC 3.- (Hydrolases)
SB  - IM
MH  - Animals
MH  - Female
MH  - Goats
MH  - Haemonchiasis/*immunology
MH  - Haemonchus/*immunology
MH  - Helminth Proteins/*immunology
MH  - Host-Parasite Interactions/*immunology
MH  - Hydrolases/*immunology
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC7338770
OTO - NOTNLM
OT  - H. contortus
OT  - excretory and secretory protein
OT  - immunomodulator
OT  - parasite-host interaction
OT  - alpha/beta-hydrolase
EDAT- 2020/07/23 06:00
MHDA- 2021/04/07 06:00
PMCR- 2020/01/01
CRDT- 2020/07/23 06:00
PHST- 2020/01/10 00:00 [received]
PHST- 2020/05/29 00:00 [accepted]
PHST- 2020/07/23 06:00 [entrez]
PHST- 2020/07/23 06:00 [pubmed]
PHST- 2021/04/07 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2020.01388 [doi]
PST - epublish
SO  - Front Immunol. 2020 Jun 30;11:1388. doi: 10.3389/fimmu.2020.01388. eCollection 
      2020.