PMID- 32695125
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 11
DP  - 2020
TI  - Chromosome Painting Based on Bulked Oligonucleotides in Cotton.
PG  - 802
LID - 10.3389/fpls.2020.00802 [doi]
LID - 802
AB  - Chromosome painting is one of the key technologies in cytogenetic research, which 
      can accurately identify chromosomes or chromosome regions. Oligonucleotide 
      (oligo) probes designed based on genome sequences have both flexibility and 
      specificity, which would be ideal probes for fluorescence in situ hybridization 
      (FISH) analysis of genome structure. In this study, the bulked oligos of the two 
      arms of chromosome seven of cotton were developed based on the genome sequence of 
      Gossypium raimondii (DD, 2n = 2x = 26), and each arm contains 12,544 oligos. 
      Chromosome seven was easily identified in both D genome and AD genome cotton 
      species using the bulked chromosome-specific painting probes. Together with 45S 
      ribosomal DNA (rDNA) probe, the chromosome-specific painting probe was also 
      successfully used to correct the chromosomal localization of 45S rDNA in G. 
      raimondii. The study reveals that bulked oligos specific to a chromosome is a 
      useful tool for chromosome painting in cotton.
CI  - Copyright (c) 2020 Liu, Wang, Wei, Liu, Lu, Liu, Zhang and Peng.
FAU - Liu, Yuling
AU  - Liu Y
AD  - Anyang Institute of Technology, Anyang, China.
FAU - Wang, Xiuyuan
AU  - Wang X
AD  - Anyang Institute of Technology, Anyang, China.
FAU - Wei, Yangyang
AU  - Wei Y
AD  - Anyang Institute of Technology, Anyang, China.
FAU - Liu, Zhen
AU  - Liu Z
AD  - Anyang Institute of Technology, Anyang, China.
FAU - Lu, Quanwei
AU  - Lu Q
AD  - Anyang Institute of Technology, Anyang, China.
FAU - Liu, Fang
AU  - Liu F
AD  - State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese 
      Academy of Agricultural Sciences, Anyang, China.
FAU - Zhang, Tao
AU  - Zhang T
AD  - Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant 
      Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop 
      Genomics and Molecular Breeding, Agricultural College of Yangzhou University, 
      Yangzhou, China.
FAU - Peng, Renhai
AU  - Peng R
AD  - Anyang Institute of Technology, Anyang, China.
LA  - eng
PT  - Journal Article
DEP - 20200630
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC7338755
OTO - NOTNLM
OT  - 45S rDNA
OT  - Gossypium raimondii
OT  - chromosome painting
OT  - fluorescence in situ hybridization
OT  - oligonucleotide
EDAT- 2020/07/23 06:00
MHDA- 2020/07/23 06:01
PMCR- 2020/01/01
CRDT- 2020/07/23 06:00
PHST- 2020/03/03 00:00 [received]
PHST- 2020/05/19 00:00 [accepted]
PHST- 2020/07/23 06:00 [entrez]
PHST- 2020/07/23 06:00 [pubmed]
PHST- 2020/07/23 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2020.00802 [doi]
PST - epublish
SO  - Front Plant Sci. 2020 Jun 30;11:802. doi: 10.3389/fpls.2020.00802. eCollection 
      2020.