PMID- 32698859
OWN - NLM
STAT- MEDLINE
DCOM- 20210329
LR  - 20210329
IS  - 1756-9966 (Electronic)
IS  - 0392-9078 (Print)
IS  - 0392-9078 (Linking)
VI  - 39
IP  - 1
DP  - 2020 Jul 22
TI  - Human umbilical cord mesenchymal stem cells-derived exosomes deliver microRNA-375 
      to downregulate ENAH and thus retard esophageal squamous cell carcinoma 
      progression.
PG  - 140
LID - 10.1186/s13046-020-01631-w [doi]
LID - 140
AB  - BACKGROUND: Exosomal microRNAs (miRNAs or miRs) from bone marrow-derived 
      mesenchymal stem cells (UCMSCs) have emerged as promising therapeutic strategies 
      for cancer treatment. The current study aimed to elucidate the underlying 
      mechanism of human umbilical cord mesenchymal stem cells (hUCMSCs)-derived 
      exosomal miR-375 in esophageal squamous cell carcinoma (ESCC). METHODS: After 
      determining the expression of miR-375 and its putative target enabled homolog 
      (ENAH) in ESCC tissues and cells, we tested effects of their altered expression 
      on ESCC proliferation, invasion, migration, and tumorsphere formation was 
      subsequently measured. Transfected hUCMSCs-derived exosomes (hUCMSCs-exo) were 
      isolated and co-cultured with ESCC cells to measure the effects of miR-375 
      delivered by hUCMSCs-exo on ESCC development. Finally, we investigated the effect 
      of miR-375 on tumor growth in vivo. RESULTS: The expression of miR-375 was 
      reduced, while the expression of ENAH was elevated in ESCC. ENAH was identified 
      as a target gene of miR-375. Elevated miR-375 or depleted ENAH expression 
      inhibited ESCC cell proliferation, invasion, migration, tumorsphere formation, 
      and promoted apoptosis. Moreover, miR-375 delivered by hUCMSCs-exo could suppress 
      ESCC cell proliferation, invasion, migration, tumorsphere formation, but promoted 
      apoptosis in vitro, as well as inhibiting tumor growth in vivo. CONCLUSIONS: 
      Taken together, hUCMSCs-exo can deliver miR-375 to suppress ENAH expression and 
      subsequently inhibit the initiation and progression of ESCC.
FAU - He, Zhanfeng
AU  - He Z
AD  - Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou 
      University, No. 1, Jianshe East Road, Zhengzhou, 450052, Henan Province, People's 
      Republic of China.
FAU - Li, Weihao
AU  - Li W
AD  - Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou 
      University, No. 1, Jianshe East Road, Zhengzhou, 450052, Henan Province, People's 
      Republic of China.
FAU - Zheng, Tianliang
AU  - Zheng T
AD  - Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou 
      University, No. 1, Jianshe East Road, Zhengzhou, 450052, Henan Province, People's 
      Republic of China.
FAU - Liu, Donglei
AU  - Liu D
AD  - Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou 
      University, No. 1, Jianshe East Road, Zhengzhou, 450052, Henan Province, People's 
      Republic of China.
FAU - Zhao, Song
AU  - Zhao S
AD  - Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou 
      University, No. 1, Jianshe East Road, Zhengzhou, 450052, Henan Province, People's 
      Republic of China. zhaosong1963@yeah.net.
LA  - eng
PT  - Journal Article
DEP - 20200722
PL  - England
TA  - J Exp Clin Cancer Res
JT  - Journal of experimental & clinical cancer research : CR
JID - 8308647
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Enah protein, human)
RN  - 0 (MIRN375 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Microfilament Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Cell Proliferation
MH  - Esophageal Neoplasms/genetics/metabolism/pathology/*therapy
MH  - Esophageal Squamous Cell Carcinoma/genetics/metabolism/pathology/therapy
MH  - Exosomes/*genetics
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/*administration & dosage/genetics
MH  - Microfilament Proteins/genetics/*metabolism
MH  - Middle Aged
MH  - Prognosis
MH  - Survival Rate
MH  - Tumor Cells, Cultured
MH  - Umbilical Cord/cytology
MH  - Xenograft Model Antitumor Assays
PMC - PMC7374920
OTO - NOTNLM
OT  - Enabled homolog
OT  - Esophageal squamous cell carcinoma
OT  - Exosomes
OT  - Human umbilical cord mesenchymal stem cells
OT  - microRNA-375
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/07/24 06:00
MHDA- 2021/03/30 06:00
PMCR- 2020/07/22
CRDT- 2020/07/24 06:00
PHST- 2019/12/25 00:00 [received]
PHST- 2020/06/29 00:00 [accepted]
PHST- 2020/07/24 06:00 [entrez]
PHST- 2020/07/24 06:00 [pubmed]
PHST- 2021/03/30 06:00 [medline]
PHST- 2020/07/22 00:00 [pmc-release]
AID - 10.1186/s13046-020-01631-w [pii]
AID - 1631 [pii]
AID - 10.1186/s13046-020-01631-w [doi]
PST - epublish
SO  - J Exp Clin Cancer Res. 2020 Jul 22;39(1):140. doi: 10.1186/s13046-020-01631-w.