PMID- 32699287
OWN - NLM
STAT- MEDLINE
DCOM- 20201210
LR  - 20210722
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Jul 22
TI  - Hydrogen-rich water reduces inflammatory responses and prevents apoptosis of 
      peripheral blood cells in healthy adults: a randomized, double-blind, controlled 
      trial.
PG  - 12130
LID - 10.1038/s41598-020-68930-2 [doi]
LID - 12130
AB  - The evidence for the beneficial effects of drinking hydrogen-water (HW) is rare. 
      We aimed to investigate the effects of HW consumption on oxidative stress and 
      immune functions in healthy adults using systemic approaches of biochemical, 
      cellular, and molecular nutrition. In a randomized, double-blind, 
      placebo-controlled study, healthy adults (20-59 y) consumed either 1.5 L/d of HW 
      (n = 20) or plain water (PW, n = 18) for 4 weeks. The changes from baseline to 
      the 4th week in serum biological antioxidant potential (BAP), derivatives of 
      reactive oxygen, and 8-Oxo-2'-deoxyguanosine did not differ between groups; 
      however, in those aged >/= 30 y, BAP increased greater in the HW group than the PW 
      group. Apoptosis of peripheral blood mononuclear cells (PBMCs) was significantly 
      less in the HW group. Flow cytometry analysis of CD4(+), CD8(+), CD20(+), CD14(+) 
      and CD11b(+) cells showed that the frequency of CD14(+) cells decreased in the HW 
      group. RNA-sequencing analysis of PBMCs demonstrated that the transcriptomes of 
      the HW group were clearly distinguished from those of the PW group. Most notably, 
      transcriptional networks of inflammatory responses and NF-kappaB signaling were 
      significantly down-regulated in the HW group. These finding suggest HW increases 
      antioxidant capacity thereby reducing inflammatory responses in healthy adults.
FAU - Sim, Minju
AU  - Sim M
AD  - Department of Food and Nutrition, Seoul National University, Seoul, 08826, 
      Republic of Korea.
FAU - Kim, Chong-Su
AU  - Kim CS
AD  - Department of Food and Nutrition, Seoul National University, Seoul, 08826, 
      Republic of Korea.
FAU - Shon, Woo-Jeong
AU  - Shon WJ
AD  - Department of Food and Nutrition, Seoul National University, Seoul, 08826, 
      Republic of Korea.
FAU - Lee, Young-Kwan
AU  - Lee YK
AD  - Department of Biomedical Sciences, Seoul National University College of Medicine, 
      Seoul, 03080, Republic of Korea.
FAU - Choi, Eun Young
AU  - Choi EY
AD  - Department of Biomedical Sciences, Seoul National University College of Medicine, 
      Seoul, 03080, Republic of Korea.
FAU - Shin, Dong-Mi
AU  - Shin DM
AD  - Department of Food and Nutrition, Seoul National University, Seoul, 08826, 
      Republic of Korea. shindm@snu.ac.kr.
AD  - Research Institute of Human Ecology, Seoul National University, Seoul, 08826, 
      Republic of Korea. shindm@snu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20200722
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antioxidants)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (NF-kappa B)
RN  - 059QF0KO0R (Water)
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Adult
MH  - Antioxidants/analysis/metabolism
MH  - *Apoptosis/drug effects
MH  - Cluster Analysis
MH  - Double-Blind Method
MH  - Down-Regulation/drug effects
MH  - Female
MH  - Healthy Volunteers
MH  - Humans
MH  - Hydrogen/administration & dosage/*chemistry/pharmacology
MH  - Leukocytes, Mononuclear/cytology/drug effects/*metabolism
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Male
MH  - Middle Aged
MH  - NF-kappa B/metabolism
MH  - Placebo Effect
MH  - Transcriptome
MH  - Water/*administration & dosage/chemistry
MH  - Young Adult
PMC - PMC7376192
COIS- The authors declare no competing interests.
EDAT- 2020/07/24 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/07/22
CRDT- 2020/07/24 06:00
PHST- 2019/09/01 00:00 [received]
PHST- 2020/04/10 00:00 [accepted]
PHST- 2020/07/24 06:00 [entrez]
PHST- 2020/07/24 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/07/22 00:00 [pmc-release]
AID - 10.1038/s41598-020-68930-2 [pii]
AID - 68930 [pii]
AID - 10.1038/s41598-020-68930-2 [doi]
PST - epublish
SO  - Sci Rep. 2020 Jul 22;10(1):12130. doi: 10.1038/s41598-020-68930-2.