PMID- 32702099
OWN - NLM
STAT- MEDLINE
DCOM- 20220519
LR  - 20220716
IS  - 1537-6613 (Electronic)
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 225
IP  - 10
DP  - 2022 May 16
TI  - An Exaggerated Monocyte-Derived Cytokine Response to Candida Hyphae in Patients 
      With Recurrent Vulvovaginal Candidiasis.
PG  - 1796-1806
LID - 10.1093/infdis/jiaa444 [doi]
AB  - BACKGROUND: Recurrent vulvovaginal candidiasis (RVVC) affects up to 8% of women. 
      The immunopathogenesis is poorly understood but it has been suggested that RVVC 
      might be due to dysregulated innate immune response. The aim of this study was to 
      compare cytokine profiles in stimulated primary mononuclear cells (PBMCs) from 
      RVVC and healthy individuals. METHODS: PBMCs isolated from RVVC patients (n = 24) 
      and healthy volunteers (n = 30) were stimulated with unspecific and 
      pathogen-specific antigens. Cytokine production was assessed after 24 hours, 48 
      hours, and 7 days using ELISA. RESULTS: No significant differences in cytokine 
      production were found in T helper 1 (Th1), Th2, and Th17 immunity in response to 
      both unspecific and pathogen-specific stimulations. Tumor necrosis factor-alpha 
      (TNF-alpha) production in response to C. albicans hyphae was significantly higher in 
      patients than controls and within the patient group, a significant positive 
      correlation was found between interleukin-1beta (IL-1beta) and both TNF-alpha and IL-6. 
      Both IL-1beta/IL-1Ra and TNF-alpha/IL-10 ratios in Candida hyphae-stimulated PBMCs were 
      significantly higher in patients than controls. CONCLUSIONS: Women affected by 
      RVVC showed increased monocytes-derived cytokine production, which might 
      contribute to an exaggerated vaginal immune response to Candida hyphae. RVVC 
      patients show no defective Th-dependent adaptive immune response upon Candida 
      stimulation.
CI  - (c) The Author(s) 2020. Published by Oxford University Press for the Infectious 
      Diseases Society of America.
FAU - Rosati, Diletta
AU  - Rosati D
AUID- ORCID: 0000-0003-2992-2503
AD  - Department of Internal Medicine, Radboud University Medical Center, Nijmegen, the 
      Netherlands.
AD  - Radboud Center for Infectious Diseases, Radboud University Medical Center, 
      Nijmegen, the Netherlands.
FAU - Bruno, Mariolina
AU  - Bruno M
AD  - Department of Internal Medicine, Radboud University Medical Center, Nijmegen, the 
      Netherlands.
AD  - Radboud Center for Infectious Diseases, Radboud University Medical Center, 
      Nijmegen, the Netherlands.
FAU - Jaeger, Martin
AU  - Jaeger M
AD  - Department of Internal Medicine, Radboud University Medical Center, Nijmegen, the 
      Netherlands.
AD  - Radboud Center for Infectious Diseases, Radboud University Medical Center, 
      Nijmegen, the Netherlands.
FAU - Kullberg, Bart-Jan
AU  - Kullberg BJ
AD  - Department of Internal Medicine, Radboud University Medical Center, Nijmegen, the 
      Netherlands.
AD  - Radboud Center for Infectious Diseases, Radboud University Medical Center, 
      Nijmegen, the Netherlands.
FAU - van de Veerdonk, Frank
AU  - van de Veerdonk F
AD  - Department of Internal Medicine, Radboud University Medical Center, Nijmegen, the 
      Netherlands.
AD  - Radboud Center for Infectious Diseases, Radboud University Medical Center, 
      Nijmegen, the Netherlands.
FAU - Netea, Mihai G
AU  - Netea MG
AD  - Department of Internal Medicine, Radboud University Medical Center, Nijmegen, the 
      Netherlands.
AD  - Radboud Center for Infectious Diseases, Radboud University Medical Center, 
      Nijmegen, the Netherlands.
AD  - Department for Genomics and Immunoregulation, Life and Medical Sciences 
      Institute, University of Bonn, Bonn, Germany.
FAU - Ten Oever, Jaap
AU  - Ten Oever J
AUID- ORCID: 0000-0003-2397-0125
AD  - Department of Internal Medicine, Radboud University Medical Center, Nijmegen, the 
      Netherlands.
AD  - Radboud Center for Infectious Diseases, Radboud University Medical Center, 
      Nijmegen, the Netherlands.
LA  - eng
GR  - 833247/ERC_/European Research Council/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Cytokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Candida
MH  - Candida albicans/physiology
MH  - *Candidiasis, Vulvovaginal
MH  - Cytokines
MH  - Female
MH  - Humans
MH  - Hyphae
MH  - Monocytes
MH  - Tumor Necrosis Factor-alpha
PMC - PMC9113504
OTO - NOTNLM
OT  - Candida albicans
OT  - RVVC
OT  - cytokines
OT  - immune response
OT  - innate immunity
OT  - recurrent vulvovaginal candidiasis
EDAT- 2020/07/24 06:00
MHDA- 2022/05/20 06:00
PMCR- 2020/07/23
CRDT- 2020/07/24 06:00
PHST- 2020/04/29 00:00 [received]
PHST- 2020/07/23 00:00 [accepted]
PHST- 2020/07/24 06:00 [pubmed]
PHST- 2022/05/20 06:00 [medline]
PHST- 2020/07/24 06:00 [entrez]
PHST- 2020/07/23 00:00 [pmc-release]
AID - 5875655 [pii]
AID - jiaa444 [pii]
AID - 10.1093/infdis/jiaa444 [doi]
PST - ppublish
SO  - J Infect Dis. 2022 May 16;225(10):1796-1806. doi: 10.1093/infdis/jiaa444.