PMID- 32702113
OWN - NLM
STAT- MEDLINE
DCOM- 20220201
LR  - 20220201
IS  - 1537-6613 (Electronic)
IS  - 0022-1899 (Linking)
VI  - 223
IP  - 5
DP  - 2021 Mar 3
TI  - Signaling Lymphocytic Activation Molecule Family-7 Alleviates Corneal 
      Inflammation by Promoting M2 Polarization.
PG  - 854-865
LID - 10.1093/infdis/jiaa445 [doi]
AB  - BACKGROUND: Signaling lymphocytic activation molecule family-7 (SLAMF7) functions 
      as an immune checkpoint molecule on macrophages in antitumor immunity. However, 
      its role in bacterial infection remains largely unknown. METHODS: Bone 
      marrow-derived macrophages (BMDMs) isolated from wild-type (WT) or SLAMF7 
      knockout (KO) mice were infected with bacteria or treated with 
      lipopolysaccharide/interferon-gamma to investigate the expression and function of 
      SLAMF7 in macrophage polarization. A Pseudomonas aeruginosa keratitis murine 
      model was established to explore the effect of SLAMF7 on P. aeruginosa keratitis 
      using WT vs SLAMF7 KO mice, or recombinant SLAMF7 vs phosphate-buffered 
      saline-treated mice, respectively. RESULTS: SLAMF7 expression was enhanced on 
      M1-polarized or bacterial-infected macrophages, and infiltrating macrophages in 
      P. aeruginosa-infected mouse corneas. SLAMF7 promoted M2 polarization by inducing 
      STAT6 activation. In vivo data showed that SLAMF7 KO aggravated, while treatment 
      with recombinant SLAMF7 alleviated, corneal inflammation and disease severity. In 
      addition, blockage of M2 polarization by Arg-1 inhibitor abrogated the effect of 
      recombinant SLAMF7 in disease progression. CONCLUSIONS: SLAMF7 expression in 
      macrophages was induced upon M1 polarization or bacterial infection and 
      alleviated corneal inflammation and disease progression of P. aeruginosa 
      keratitis by promoting M2 polarization. These findings may provide a potential 
      strategy for the treatment of P. aeruginosa keratitis.
CI  - (c) The Author(s) 2020. Published by Oxford University Press for the Infectious 
      Diseases Society of America. All rights reserved. For permissions, e-mail: 
      journals.permissions@oup.com.
FAU - Zhu, Siying
AU  - Zhu S
AD  - Program of Infection and Immunology, The Fifth Affiliated Hospital of Sun Yat-sen 
      University, Zhongshan School of Medicine, Sun Yat-sen University, Guangdong, 
      China.
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou, China.
FAU - Chen, Yu
AU  - Chen Y
AD  - Program of Infection and Immunology, The Fifth Affiliated Hospital of Sun Yat-sen 
      University, Zhongshan School of Medicine, Sun Yat-sen University, Guangdong, 
      China.
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou, China.
FAU - Lao, Juanfeng
AU  - Lao J
AD  - Program of Infection and Immunology, The Fifth Affiliated Hospital of Sun Yat-sen 
      University, Zhongshan School of Medicine, Sun Yat-sen University, Guangdong, 
      China.
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou, China.
FAU - Wu, Chenglin
AU  - Wu C
AD  - Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Zhan, Xiaoxia
AU  - Zhan X
AD  - Department of Laboratory Medicine, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Wu, Yongjian
AU  - Wu Y
AD  - Program of Infection and Immunology, The Fifth Affiliated Hospital of Sun Yat-sen 
      University, Zhongshan School of Medicine, Sun Yat-sen University, Guangdong, 
      China.
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou, China.
FAU - Shang, Yuqi
AU  - Shang Y
AD  - Department of Infectious Diseases, The Fifth Affiliated Hospital, Sun Yat-sen 
      University, Zhuhai, China.
FAU - Zou, Zhengyu
AU  - Zou Z
AD  - Program of Infection and Immunology, The Fifth Affiliated Hospital of Sun Yat-sen 
      University, Zhongshan School of Medicine, Sun Yat-sen University, Guangdong, 
      China.
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou, China.
FAU - Zhou, Jinyu
AU  - Zhou J
AD  - Program of Infection and Immunology, The Fifth Affiliated Hospital of Sun Yat-sen 
      University, Zhongshan School of Medicine, Sun Yat-sen University, Guangdong, 
      China.
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou, China.
FAU - Ji, Xiang
AU  - Ji X
AD  - Program of Infection and Immunology, The Fifth Affiliated Hospital of Sun Yat-sen 
      University, Zhongshan School of Medicine, Sun Yat-sen University, Guangdong, 
      China.
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou, China.
FAU - Huang, Xi
AU  - Huang X
AD  - Department of Infectious Diseases, The Fifth Affiliated Hospital, Sun Yat-sen 
      University, Zhuhai, China.
FAU - Shi, Xiaomin
AU  - Shi X
AD  - Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Wu, Minhao
AU  - Wu M
AD  - Program of Infection and Immunology, The Fifth Affiliated Hospital of Sun Yat-sen 
      University, Zhongshan School of Medicine, Sun Yat-sen University, Guangdong, 
      China.
AD  - Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of 
      Education, Guangzhou, China.
AD  - Guangdong Engineering and Technology Research Center for Disease-Model Animals, 
      Sun Yat-sen University, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Signaling Lymphocytic Activation Molecule Family)
RN  - 0 (Slamf7 protein, mouse)
SB  - IM
MH  - Animals
MH  - Cell Polarity
MH  - *Cornea/physiopathology
MH  - Disease Progression
MH  - *Inflammation
MH  - *Keratitis/drug therapy/microbiology
MH  - Macrophages/*cytology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Pseudomonas Infections
MH  - Pseudomonas aeruginosa
MH  - Signal Transduction
MH  - Signaling Lymphocytic Activation Molecule Family/*genetics
OTO - NOTNLM
OT  - SLAMF7
OT  - M1/M2 polarization
OT  - corneal inflammation
EDAT- 2020/07/24 06:00
MHDA- 2022/02/02 06:00
CRDT- 2020/07/24 06:00
PHST- 2020/04/30 00:00 [received]
PHST- 2020/07/11 00:00 [revised]
PHST- 2020/07/17 00:00 [accepted]
PHST- 2020/07/24 06:00 [pubmed]
PHST- 2022/02/02 06:00 [medline]
PHST- 2020/07/24 06:00 [entrez]
AID - 5875656 [pii]
AID - 10.1093/infdis/jiaa445 [doi]
PST - ppublish
SO  - J Infect Dis. 2021 Mar 3;223(5):854-865. doi: 10.1093/infdis/jiaa445.