PMID- 32702419
OWN - NLM
STAT- MEDLINE
DCOM- 20210412
LR  - 20210412
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 164
DP  - 2020 Dec 1
TI  - Cardiac ECM/chitosan/alginate ternary scaffolds for cardiac tissue engineering 
      application.
PG  - 389-402
LID - S0141-8130(20)33887-3 [pii]
LID - 10.1016/j.ijbiomac.2020.07.134 [doi]
AB  - Since cardiovascular diseases are the number one cause of death in worldwide, and 
      the traditional treatments have limitations, the emergence of cardiac tissue 
      engineering (CTE) can be a promising approach. In this study, scaffold 
      fabrication from the solubilized cardiac extracellular matrix (ECM) accompanied 
      with alginate and chitosan for CTE was carried out. The influence of blending 
      ratios on chemical and physical properties of the scaffolds including FTIR 
      spectroscopy, porosity, pore size, and their mechanical properties were 
      investigated. The porosity of scaffolds was more than 96% with very high swelling 
      rate while maintaining their stability in PBS solution. Blending ECM with 
      chitosan and alginate significantly improve the tensile strength of ECM. FTIR 
      spectrum of scaffolds demonstrated interaction of solubilized ECM with two 
      opposite-charged polysaccharides. The proliferation of human mesenchymal stem 
      cells (hMSCs) on the ternary scaffolds using MTS assay, revealed that blending 
      ECM with polysaccharides at ratio of 75: 25 (E75/P25) led to improve the 
      proliferation of hMSCs on scaffolds. Scanning electron microscope (SEM) revealed 
      the porous structure and the presence of hMSCs cells inside the pores. In 
      addition, histological analysis confirmed that cardiomyocyte penetration inside 
      scaffolds after 7 days of culture. The immunofluorescence staining revealed that 
      higher expression of cardiac marker (cTnT) in ternary scaffold in comparison with 
      ECM(.)
CI  - Copyright (c) 2020. Published by Elsevier B.V.
FAU - Tamimi, Maryam
AU  - Tamimi M
AD  - Hard Tissue Engineering Research Center, Tissue Engineering and Regenerative 
      Medicine Institute, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
FAU - Rajabi, Sarah
AU  - Rajabi S
AD  - Department of Cell Engineering, Cell Science Research Center, Royan Institute for 
      Stem Cell Biology and Technology, ACECR, Tehran, Iran. Electronic address: 
      Srajabi@royaninstitute.org.
FAU - Pezeshki-Modaress, Mohamad
AU  - Pezeshki-Modaress M
AD  - Burn Research Center, Iran University of Medical Sciences, Tehran, Iran. 
      Electronic address: Pezeshkimodaress.m@iums.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20200720
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Alginates)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Alginates/*chemistry
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Chemical Phenomena
MH  - Chitosan/*chemistry
MH  - Extracellular Matrix/*metabolism
MH  - Humans
MH  - Hydrolysis
MH  - Immunohistochemistry
MH  - Mechanical Phenomena
MH  - Myocytes, Cardiac/*metabolism
MH  - *Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - Alginate
OT  - Cardiac tissue engineering
OT  - Chitosan
OT  - Extracellular matrix
OT  - Scaffold
EDAT- 2020/07/24 06:00
MHDA- 2021/04/13 06:00
CRDT- 2020/07/24 06:00
PHST- 2020/02/06 00:00 [received]
PHST- 2020/04/18 00:00 [revised]
PHST- 2020/07/11 00:00 [accepted]
PHST- 2020/07/24 06:00 [pubmed]
PHST- 2021/04/13 06:00 [medline]
PHST- 2020/07/24 06:00 [entrez]
AID - S0141-8130(20)33887-3 [pii]
AID - 10.1016/j.ijbiomac.2020.07.134 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2020 Dec 1;164:389-402. doi: 10.1016/j.ijbiomac.2020.07.134. 
      Epub 2020 Jul 20.