PMID- 32706998
OWN - NLM
STAT- MEDLINE
DCOM- 20211110
LR  - 20211110
IS  - 1557-8852 (Electronic)
IS  - 1084-9785 (Linking)
VI  - 36
IP  - 4
DP  - 2021 May
TI  - Long Noncoding RNA HNF1A-AS1 Regulates Osteosarcoma Advancement Through 
      Modulating the miR-32-5p/HMGB1 Axis.
PG  - 371-381
LID - 10.1089/cbr.2019.3486 [doi]
AB  - Background: Osteosarcoma (OS) is a primary malignant tumor in children and 
      adolescents. Long noncoding RNA HNF1A antisense RNA 1 (HNF1A-AS1) is connected 
      with OS development. However, there are few reports on the role and mechanism of 
      HNF1A-AS1 in OS. Materials and Methods: Quantitative real-time polymerase chain 
      reaction (qRT-PCR) was employed to assess the expression of HNF1A-AS1, miR-32-5p, 
      and high-mobility group protein B1 (HMGB1). Western blot analysis was performed 
      to detect the protein level of HMGB1. 
      3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony 
      formation, transwell, or flow cytometer assays were applied to determine the 
      proliferation, migration, invasion, and apoptosis of OS cells. The interaction 
      between HNF1A-AS1 and miR-32-5p or HMGB1 was predicted by the starBase database 
      and confirmed by dual-luciferase reporter assay. Enzyme-linked immunosorbent 
      assay was employed to analyze levels of HMGB1 in the OS cell supernatant. 
      Results: HNF1A-AS1 and HMGB1 were upregulated, while miR-32-5p was downregulated, 
      in OS tissues and cells. Functionally, HNF1A-AS1 depletion induced apoptosis and 
      impeded proliferation, migration, and invasion of OS cells. Interestingly, 
      HNF1A-AS1 bound to miR-32-5p to regulate the expression of HMGB1. Furthermore, 
      miR-32-5p knockdown overturned the effects of HNF1A-AS1 knockdown on apoptosis, 
      proliferation, migration, and invasion of OS cells. In addition, the effects of 
      HNF1A-AS1 silencing on the malignant behaviors of OS cells were reserved by HMGB1 
      overexpression. In addition, HNF1A-AS1 regulated the HMGB1 level in the OS cell 
      supernatant through the miR-32-5p/HMGB1 axis. Conclusion: Downregulation of 
      HNF1A-AS1 blocked OS progression through the miR-32-5p/HMGB1 axis, which provides 
      a possible target and prognostic biomarker for treatment of OS.
FAU - Lou, Pan
AU  - Lou P
AD  - Department of Spinal Surgery, Jingmen No. 1 People's Hospital, Jingmen, China.
FAU - Ding, Tao
AU  - Ding T
AD  - Department of Reproductive Medicine, Jingmen No. 2 People's Hospital, Jingmen, 
      China.
FAU - Zhan, Xu
AU  - Zhan X
AD  - Department of Spinal Surgery, Jingmen No. 1 People's Hospital, Jingmen, China.
LA  - eng
PT  - Journal Article
DEP - 20200720
PL  - United States
TA  - Cancer Biother Radiopharm
JT  - Cancer biotherapy & radiopharmaceuticals
JID - 9605408
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, human)
RN  - 0 (MIRN32 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (long non-coding RNA HNF1A-AS1, human)
SB  - IM
MH  - Bone Neoplasms/genetics/*metabolism/pathology
MH  - HMGB1 Protein/genetics/*metabolism
MH  - Humans
MH  - MicroRNAs/genetics/*metabolism/pharmacology
MH  - Osteosarcoma/*genetics/*metabolism/pathology
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - HMGB1
OT  - HNF1A-AS1
OT  - OS
OT  - miR-32-5p
EDAT- 2020/07/25 06:00
MHDA- 2021/11/11 06:00
CRDT- 2020/07/25 06:00
PHST- 2020/07/25 06:00 [pubmed]
PHST- 2021/11/11 06:00 [medline]
PHST- 2020/07/25 06:00 [entrez]
AID - 10.1089/cbr.2019.3486 [doi]
PST - ppublish
SO  - Cancer Biother Radiopharm. 2021 May;36(4):371-381. doi: 10.1089/cbr.2019.3486. 
      Epub 2020 Jul 20.