PMID- 32707879
OWN - NLM
STAT- MEDLINE
DCOM- 20210310
LR  - 20211204
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 9
IP  - 8
DP  - 2020 Jul 22
TI  - RTH-149 Cell Line, a Useful Tool to Decipher Molecular Mechanisms Related to Fish 
      Nutrition.
LID - 10.3390/cells9081754 [doi]
LID - 1754
AB  - Nowadays, aquaculture provides more than 50% of fish consumed worldwide but faces 
      new issues that challenge its sustainability. One of them relies on the 
      replacement of fish meal (FM) in aquaculture feeds by other protein sources 
      without deeply affecting the whole organism's homeostasis. Multiple strategies 
      have already been tested using in vivo approaches, but they hardly managed to 
      cope with the multifactorial problems related to the complexities of fish biology 
      together with new feed formulations. In this context, rainbow trout (RT) is 
      particularly concerned by these problems, since, as a carnivorous fish, dietary 
      proteins provide the amino acids required to supply most of its energetic 
      metabolism. Surprisingly, we noticed that in vitro approaches considering RT cell 
      lines as models to study RT amino acid metabolism were never previously used. 
      Therefore, we decided to investigate if, and how, three major pathways described, 
      in other species, to be regulated by amino acid and to control cellular 
      homeostasis were functional in a RT cell line called RTH-149-namely, the 
      mechanistic Target Of Rapamycin (mTOR), autophagy and the general control 
      nonderepressible 2 (GCN2) pathways. Our results not only demonstrated that these 
      three pathways were functional in RTH-149 cells, but they also highlighted some 
      RT specificities with respect to the time response, amino acid dependencies and 
      the activation levels of their downstream targets. Altogether, this article 
      demonstrated, for the first time, that RT cell lines could represent an 
      interesting alternative of in vivo experimentations for the study of fish 
      nutrition-related questions.
FAU - Morin, Guillaume
AU  - Morin G
AD  - Universite de Pau et des Pays de l'Adour, E2S UPPA, INRAE, NUMEA, 64310 
      Saint-Pee-sur-Nivelle, France.
FAU - Pinel, Karine
AU  - Pinel K
AD  - Universite de Pau et des Pays de l'Adour, E2S UPPA, INRAE, NUMEA, 64310 
      Saint-Pee-sur-Nivelle, France.
FAU - Dias, Karine
AU  - Dias K
AD  - Universite de Pau et des Pays de l'Adour, E2S UPPA, INRAE, NUMEA, 64310 
      Saint-Pee-sur-Nivelle, France.
FAU - Seiliez, Iban
AU  - Seiliez I
AUID- ORCID: 0000-0002-2202-1756
AD  - Universite de Pau et des Pays de l'Adour, E2S UPPA, INRAE, NUMEA, 64310 
      Saint-Pee-sur-Nivelle, France.
FAU - Beaumatin, Florian
AU  - Beaumatin F
AUID- ORCID: 0000-0001-6017-7172
AD  - Universite de Pau et des Pays de l'Adour, E2S UPPA, INRAE, NUMEA, 64310 
      Saint-Pee-sur-Nivelle, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200722
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Amino Acids)
RN  - 0 (Culture Media)
RN  - 886U3H6UFF (Chloroquine)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acids/metabolism/pharmacology
MH  - Animals
MH  - Aquaculture/methods
MH  - Autophagy/*drug effects/genetics
MH  - Carcinoma, Hepatocellular/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Chloroquine/pharmacology
MH  - Culture Media/chemistry
MH  - Gene Expression/drug effects
MH  - Homeostasis/drug effects
MH  - Liver Neoplasms/*metabolism/pathology
MH  - Oncorhynchus mykiss/*metabolism
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/*metabolism
PMC - PMC7463835
OTO - NOTNLM
OT  - GCN2 pathway
OT  - RTH-149 cell line
OT  - amino acids
OT  - aquaculture
OT  - autophagy
OT  - cell homeostasis
OT  - mTOR
OT  - nutrition
OT  - rainbow trout
COIS- The authors declare no conflict of interest.
EDAT- 2020/07/28 06:00
MHDA- 2021/03/11 06:00
PMCR- 2020/08/01
CRDT- 2020/07/26 06:00
PHST- 2020/06/22 00:00 [received]
PHST- 2020/07/19 00:00 [revised]
PHST- 2020/07/20 00:00 [accepted]
PHST- 2020/07/26 06:00 [entrez]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2021/03/11 06:00 [medline]
PHST- 2020/08/01 00:00 [pmc-release]
AID - cells9081754 [pii]
AID - cells-09-01754 [pii]
AID - 10.3390/cells9081754 [doi]
PST - epublish
SO  - Cells. 2020 Jul 22;9(8):1754. doi: 10.3390/cells9081754.