PMID- 32707944
OWN - NLM
STAT- MEDLINE
DCOM- 20210308
LR  - 20231213
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 10
IP  - 8
DP  - 2020 Jul 22
TI  - Immunoglobulin E-Binding Pattern of Canadian Peanut Allergic Children and 
      Cross-Reactivity with Almond, Hazelnut and Pistachio.
LID - 10.3390/biom10081091 [doi]
LID - 1091
AB  - Peanut allergic individuals can be both co-sensitized and co-allergic to peanut 
      and tree nuts. At the moment, standard diagnostic approaches do not always allow 
      differentiation between clinically relevant sensitization and nonsignificant 
      cross-reactions, and the responsibility of each allergen remains unclear. The 
      objective of this study was therefore to determine a peanut sensitization profile 
      in a cohort of Canadian peanut allergic children and assess the immunoglobulin E 
      (IgE) molecular cross-reactivity between peanut, almond, hazelnut and pistachio. 
      The specific IgE (sIgE) levels of each patient serum were determined by 
      ImmunoCAP, indirect ELISA and immunoblot to examine their sIgE-binding levels and 
      profiles to peanut proteins. Reciprocal inhibition ELISA and immunoblotting were 
      used to study sIgE cross-reactions between peanut and the selected tree nuts 
      using an adjusted and representative serum pool of the nine allergic patients. 
      The results showed that the prepared peanut and tree nut protein extracts allowed 
      for the detection of the majority of peanut and selected tree nut known 
      allergens. The reciprocal inhibition ELISA experiments showed limited sIgE 
      cross-reactivities between peanut and the studied tree nuts, with peanut being 
      most likely the sensitizing allergen and tree nuts the cross-reactive ones. In 
      the case of hazelnut and pistachio, a coexisting primary sensitization to 
      hazelnut and pistachio was also demonstrated in the serum pool. Reciprocal 
      inhibition immunoblotting further revealed that storage proteins (2S albumin, 7S 
      vicilin and 11S legumin) could possibly account for the observed 
      IgE-cross-reactions between peanut and the studied tree nuts in this cohort of 
      allergic individuals. It also demonstrated the importance of conformational 
      epitopes in the exhibited cross-reactions.
FAU - Pitre, Melanie
AU  - Pitre M
AD  - Saint-Hyacinthe Research and Development Centre, Agriculture and Agri-Food 
      Canada, 3600 Casavant Blvd. W. Saint-Hyacinthe, QC J2S 8E3, Canada.
FAU - L'Hocine, Lamia
AU  - L'Hocine L
AUID- ORCID: 0000-0001-9370-3925
AD  - Saint-Hyacinthe Research and Development Centre, Agriculture and Agri-Food 
      Canada, 3600 Casavant Blvd. W. Saint-Hyacinthe, QC J2S 8E3, Canada.
FAU - Achouri, Allaoua
AU  - Achouri A
AD  - Saint-Hyacinthe Research and Development Centre, Agriculture and Agri-Food 
      Canada, 3600 Casavant Blvd. W. Saint-Hyacinthe, QC J2S 8E3, Canada.
FAU - Blaquiere, Martin
AU  - Blaquiere M
AD  - Sherbrooke University Hospital Center, Hotel-Dieu de Sherbrooke, Department of 
      Pediatrics, 580 Bowen south, Sherbrooke, QC J1G 2E3, Canada.
FAU - Des Roches, Anne
AU  - Des Roches A
AD  - Immunoallergy and Rheumatology Service Department of Pediatrics Medical School 
      CHU Sainte-Justine Institution, 3175 Cote Sainte-Catherine, Montreal, QC H3T 1C5, 
      Canada.
LA  - eng
GR  - RBPI#1255/Agriculture and Agri-Food Canada/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200722
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
RN  - 0 (Allergens)
RN  - 0 (Plant Proteins)
RN  - 0 (Seed Storage Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9067-60-1 (vicilin protein, plant)
SB  - IM
MH  - Adolescent
MH  - Allergens/immunology
MH  - Arachis/*immunology
MH  - Canada
MH  - Child
MH  - Child, Preschool
MH  - Corylus/*immunology
MH  - Cross Reactions/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin E/blood/*immunology
MH  - Male
MH  - Peanut Hypersensitivity/*immunology
MH  - Pistacia/*immunology
MH  - Plant Proteins/immunology
MH  - Prunus dulcis/*immunology
MH  - Seed Storage Proteins/immunology
MH  - Legumins
PMC - PMC7464554
OTO - NOTNLM
OT  - IgE-binding
OT  - allergens
OT  - almond
OT  - cross-reactivity
OT  - hazelnut
OT  - inhibition ELISA
OT  - inhibition immunoblotting
OT  - peanut
OT  - pistachio
OT  - protein extracts
OT  - sensitization
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses or interpretation of the data; 
      in the writing of the manuscript or in the decision to publish the results.
EDAT- 2020/07/28 06:00
MHDA- 2021/03/09 06:00
PMCR- 2020/08/01
CRDT- 2020/07/26 06:00
PHST- 2020/06/22 00:00 [received]
PHST- 2020/07/17 00:00 [revised]
PHST- 2020/07/20 00:00 [accepted]
PHST- 2020/07/26 06:00 [entrez]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2021/03/09 06:00 [medline]
PHST- 2020/08/01 00:00 [pmc-release]
AID - biom10081091 [pii]
AID - biomolecules-10-01091 [pii]
AID - 10.3390/biom10081091 [doi]
PST - epublish
SO  - Biomolecules. 2020 Jul 22;10(8):1091. doi: 10.3390/biom10081091.