PMID- 32708645
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2073-4360 (Electronic)
IS  - 2073-4360 (Linking)
VI  - 12
IP  - 7
DP  - 2020 Jul 16
TI  - Effects of Electrospun Fibrous Membranes of PolyCaprolactone and 
      Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions.
LID - 10.3390/polym12071580 [doi]
LID - 1580
AB  - Polycaprolactone (PCL) is a synthetic polymer with good mechanical properties 
      that are useful to produce biomaterials of clinical application. It can be 
      successfully combined with chitosan, which enhances the biomaterial properties 
      through the modulation of molecular and cellular mechanisms. The objective of 
      this study was to evaluate the effects of the use of electrospun fibrous 
      membranes consisting of polycaprolactone (PCL) or polycaprolactone coated with 
      chitosan and poly(ethylene oxide) (PCL+CHI/PEO) on mouse skin lesions. Sixty four 
      Black-57 mice were divided into PCL and PCL+CHI/PEO groups. A 1 cm(2) lesion was 
      made on the animals' backs, and the membranes were sutured in place. The tissues 
      were extracted on the 3rd, 7th, and 14th days after the lesion. The tissues were 
      analyzed by histology with Hematoxylin and Eosin (H&E) and Sirius Red stains, 
      morphometry, immunohistochemistry, and Western blot. On the 3rd, 6th, and 9th 
      days after the lesion, the PCL+CHI/PEO group showed a higher wound-healing rate 
      (WHR). On the 3 day, the PCL+CHI/PEO group showed a greater amount of 
      inflammatory infiltrate, greater expression of proliferating cell nuclear antigen 
      (PCNA), and smooth muscle actin (alpha-SMA) (p < 0.05) compared to the PCL group. On 
      the 7th day after the lesion, the PCL+CHI/PEO group showed a greater amount of 
      inflammatory infiltrate, expression of Tumor Necrosis Factor (TNF-alpha) and PCNA (p 
      < 0.05). In addition, it showed a greater immunolabeling of Monocyte 
      Chemoattractant Protein-1 (MCP-1) and deposition of collagen fibers compared to 
      the PCL group. The PCL+CHI/PEO membrane modulated the increase in the 
      inflammatory infiltrate, the expression of MCP-1, PCNA, and alpha-SMA in lesions of 
      mice.
FAU - Zanchetta, Flavia Cristina
AU  - Zanchetta FC
AUID- ORCID: 0000-0002-5934-9683
AD  - School of Nursing, University of Campinas, Campinas CEP 13083887, Brazil.
FAU - Trinca, Rafael Bergamo
AU  - Trinca RB
AUID- ORCID: 0000-0002-8283-090X
AD  - Department of Engineering of Materials and of Bioprocess, School of Chemical 
      Engineering, University of Campinas, Campinas CEP 13083852, Brazil.
FAU - Gomes Silva, Juliany Lino
AU  - Gomes Silva JL
AUID- ORCID: 0000-0002-2930-103X
AD  - School of Nursing, University of Campinas, Campinas CEP 13083887, Brazil.
FAU - Breder, Jessica da Silva Cunha
AU  - Breder JDSC
AD  - School of Nursing, University of Campinas, Campinas CEP 13083887, Brazil.
FAU - Cantarutti, Thiago Anselmo
AU  - Cantarutti TA
AD  - School of Nursing, University of Campinas, Campinas CEP 13083887, Brazil.
FAU - Consonni, Silvio Roberto
AU  - Consonni SR
AUID- ORCID: 0000-0003-3149-021X
AD  - Department of Biochemistry and Tissue Biology, Institute of Biology, University 
      of Campinas, Campinas CEP 13083970, Brazil.
FAU - Moraes, Angela Maria
AU  - Moraes AM
AUID- ORCID: 0000-0002-5813-332X
AD  - Department of Engineering of Materials and of Bioprocess, School of Chemical 
      Engineering, University of Campinas, Campinas CEP 13083852, Brazil.
FAU - Pereira de Araujo, Eliana
AU  - Pereira de Araujo E
AD  - School of Nursing, University of Campinas, Campinas CEP 13083887, Brazil.
FAU - Saad, Mario Jose Abdalla
AU  - Saad MJA
AD  - Department of Internal Medicine, University of Campinas, Campinas CEP 13083887, 
      Brazil.
FAU - Adams, Gary G
AU  - Adams GG
AUID- ORCID: 0000-0002-0335-2162
AD  - School of Health Sciences, Faculty of Medicine, The University of Nottingham, C 
      Floor, South Block Link, Queen's Medical Centre, Nottingham NG7 2HA, UK.
FAU - Melo Lima, Maria Helena
AU  - Melo Lima MH
AUID- ORCID: 0000-0001-6521-8324
AD  - School of Nursing, University of Campinas, Campinas CEP 13083887, Brazil.
LA  - eng
GR  - 307139/2015-8 and 307829/2018-9/Conselho Nacional de Desenvolvimento Cientifico e 
      Tecnologico/
PT  - Journal Article
DEP - 20200716
PL  - Switzerland
TA  - Polymers (Basel)
JT  - Polymers
JID - 101545357
PMC - PMC7408160
OTO - NOTNLM
OT  - C57BL/6J mice
OT  - chitosan
OT  - electrospinning
OT  - polycaprolactone
OT  - wound dressing
OT  - wound healing
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/07/28 06:00
MHDA- 2020/07/28 06:01
PMCR- 2020/07/16
CRDT- 2020/07/26 06:00
PHST- 2020/06/12 00:00 [received]
PHST- 2020/07/09 00:00 [revised]
PHST- 2020/07/13 00:00 [accepted]
PHST- 2020/07/26 06:00 [entrez]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2020/07/28 06:01 [medline]
PHST- 2020/07/16 00:00 [pmc-release]
AID - polym12071580 [pii]
AID - polymers-12-01580 [pii]
AID - 10.3390/polym12071580 [doi]
PST - epublish
SO  - Polymers (Basel). 2020 Jul 16;12(7):1580. doi: 10.3390/polym12071580.