PMID- 32708891
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2077-0383 (Print)
IS  - 2077-0383 (Electronic)
IS  - 2077-0383 (Linking)
VI  - 9
IP  - 7
DP  - 2020 Jul 17
TI  - Changes in High-Density Lipoproteins Related to Outcomes in Patients with Acute 
      Stroke.
LID - 10.3390/jcm9072269 [doi]
LID - 2269
AB  - Modifications in high-density lipoprotein (HDL) particle sizes and HDL-binding 
      proteins have been reported in stroke patients. We evaluated whether the 
      lipoprotein profile, HDL composition and functionality were altered in stroke 
      patients according to their clinical outcome using the modified Rankin Score at 3 
      months. Plasma samples were obtained from stroke patients treated with 
      intravenous thrombolysis. Levels of cardiovascular and inflammatory markers in 
      plasma were measured using the Human CVD Panel 1 (Milliplex((R)) MAP). Lipoprotein 
      subfractions from plasma were quantified by non-denaturing acrylamide gel 
      electrophoresis, using the Lipoprint((R))-System (Quantimetrix((R))), and HDLs were 
      isolated by ultracentrifugation. Relative amounts of paraoxonase-1 (PON1) and 
      alpha-1 anti-trypsin (AAT) in the isolated HDLs were determined by Western blot. 
      HDL anti-inflammatory function was evaluated in human blood-brain barrier 
      endothelial cells stimulated with 100 ng/mL TNFalpha, and HDL antioxidant function 
      was evaluated via their capacity to limit copper-induced low-density lipoprotein 
      oxidation. Stroke patients with unfavorable outcomes had a lower proportion of 
      small-sized HDLs and increased plasma levels of E-selectin (SELE) and the 
      intercellular adhesion molecule 1 (ICAM1). HDLs from patients with unfavorable 
      outcomes had lower levels of PON1 and displayed a blunted capacity to reduce the 
      expression of SELE, interleukin 8 (IL8) and the monocyte chemoattractant 
      protein-1 (MCP1) mRNA induced by TNFalpha in endothelial cells. These HDLs also had a 
      reduced antioxidant capacity relative to HDLs from healthy donors. In conclusion, 
      an increased ratio of large/small HDLs with impaired anti-inflammatory and 
      antioxidant capacities was associated with unfavorable outcomes in stroke 
      patients. Alteration of HDL functionality was mainly associated with a low amount 
      of PON1 and high amount of AAT.
FAU - Varela, Lourdes M
AU  - Varela LM
AUID- ORCID: 0000-0002-8875-6042
AD  - Inserm U1148, Paris University, 75018 Paris, France.
AD  - Instituto de Biomedicina de Sevilla (IBiS)/Hospital Universitario Virgen del 
      Rocio/CSIC/Departamento de Fisiologia Medica y Biofisica-Universidad de Sevilla, 
      41013 Sevilla, Spain.
FAU - Meseguer, Elena
AU  - Meseguer E
AUID- ORCID: 0000-0002-7184-2614
AD  - Department of Neurology and Stroke Center, Paris University, 75018 Paris, France.
FAU - Lapergue, Bertrand
AU  - Lapergue B
AD  - Department of Neurology, Stroke Center, Foch Hospital, 92150 Suresnes, France.
FAU - Couret, David
AU  - Couret D
AD  - CHU de La Reunion, 97410 Saint-Pierre, France.
FAU - Amarenco, Pierre
AU  - Amarenco P
AD  - Department of Neurology and Stroke Center, Paris University, 75018 Paris, France.
FAU - Meilhac, Olivier
AU  - Meilhac O
AD  - CHU de La Reunion, 97410 Saint-Pierre, France.
AD  - Universite de La Reunion, Inserm U1188 DeTROI, F-97490 Sainte-Clotilde, France.
LA  - eng
GR  - ANR-JCJC-1105/Agence Nationale de la Recherche/
GR  - VI PPIT-US/Universidad de Sevilla/
GR  - RE0001897/European Regional Development Fund/
PT  - Journal Article
DEP - 20200717
PL  - Switzerland
TA  - J Clin Med
JT  - Journal of clinical medicine
JID - 101606588
PMC - PMC7408777
OTO - NOTNLM
OT  - HDL
OT  - PON1
OT  - blood-brain barrier (BBB) dysfunction
OT  - functional outcome
OT  - low-density lipoproteins (LDL) oxidation
OT  - stroke
COIS- The authors have no conflict of interest.
EDAT- 2020/07/28 06:00
MHDA- 2020/07/28 06:01
PMCR- 2020/07/17
CRDT- 2020/07/26 06:00
PHST- 2020/06/18 00:00 [received]
PHST- 2020/07/11 00:00 [revised]
PHST- 2020/07/16 00:00 [accepted]
PHST- 2020/07/26 06:00 [entrez]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2020/07/28 06:01 [medline]
PHST- 2020/07/17 00:00 [pmc-release]
AID - jcm9072269 [pii]
AID - jcm-09-02269 [pii]
AID - 10.3390/jcm9072269 [doi]
PST - epublish
SO  - J Clin Med. 2020 Jul 17;9(7):2269. doi: 10.3390/jcm9072269.