PMID- 32710808
OWN - NLM
STAT- MEDLINE
DCOM- 20211122
LR  - 20211122
IS  - 1521-6551 (Electronic)
IS  - 1521-6543 (Linking)
VI  - 72
IP  - 9
DP  - 2020 Sep
TI  - Identification of continuous immunoglobulin G epitopes of Dermatophagoides 
      farinae allergens by peptide microarray immunoassay.
PG  - 1976-1985
LID - 10.1002/iub.2333 [doi]
AB  - Dermatophagoides farinae, as a common house dust mite species, is one of the main 
      sources of allergens in the world. At present, Dermatophagoides farinae is found 
      to contain more than 30 groups of allergens. These allergens are used for 
      allergen-specific immunotherapy (AIT) of allergic diseases. During the AIT 
      process, immunoglobulin G (IgG) antibodies can block immunoglobulin E (IgE) 
      antibody-induced allergic reactions in the human body. One of the mechanisms may 
      be that IgG and IgE competitively bind to the same allergic protein, so it is 
      necessary to explore the binding sites (epitopes) of IgG antibodies to allergens. 
      In this study, peptide arrays were constructed to react with the serums from 
      patients with allergic asthma to find the IgG epitopes of several allergens 
      including major allergens (Der f 1, 2) and mid-tier allergens (Der f 4, 5, and 
      7), and then verified by enzyme-linked immunosorbent assay (ELISA) test. Relevant 
      epitopic sequences were located on the tertiary structure of individual 
      allergens, as reconstructed by homology modeling. One IgG epitope of Der f 1 
      (90-106aa, NVPSELDLRSLRTVTPI), five IgG epitopes of Der f 4 (61-77aa, 
      ERYQPVSYDIHTRSGDE; 193-209aa, FRSDASTHQWPDDLRSI; 226-242aa, HPFIYHETIYYGGNGIN; 
      271-287aa, LRWLRNFGTEWGLVPSG; 352-368aa, NDWVGPPTDQHGNILSV), and one IgG epitope 
      of Der f 5 (84-101aa, RYNVEIALKSNEILERDL) were identified. IgG epitopes of Der f 
      2, 7 were not found. There are overlaps between the IgG and IgE epitopes of Der f 
      1, 4, and 5. These findings not only reflect the practicality of peptide array 
      and ELISA test in the allergen IgG epitope identification, but also provide more 
      information for further understanding of the human immunological changes during 
      AIT and the molecular mechanisms of IgG blocking IgE activity.
CI  - (c) 2020 International Union of Biochemistry and Molecular Biology.
FAU - Teng, Feixiang
AU  - Teng F
AUID- ORCID: 0000-0003-4624-9584
AD  - Department of Basic Medicine, Jiangsu Vocational College of Medicine, Yancheng, 
      China.
FAU - Han, Feifei
AU  - Han F
AD  - Department of Clinical Laboratory, Wuxi People's Hospital Affiliated to Nanjing 
      Medical University, Wuxi, China.
FAU - Zhu, Xuming
AU  - Zhu X
AD  - Department of Clinical Laboratory, Wuxi People's Hospital Affiliated to Nanjing 
      Medical University, Wuxi, China.
FAU - Yu, Lili
AU  - Yu L
AD  - Department of Basic Medicine, Jiangsu Vocational College of Medicine, Yancheng, 
      China.
FAU - Gai, Dongzheng
AU  - Gai D
AD  - Department of Internal Medicine, University of Arkansas for Medical Sciences, 
      Little Rock, Arkansas, USA.
FAU - Xu, Cong
AU  - Xu C
AD  - Department of Internal Medicine, University of Arkansas for Medical Sciences, 
      Little Rock, Arkansas, USA.
FAU - Cui, Yubao
AU  - Cui Y
AUID- ORCID: 0000-0001-7736-8012
AD  - Department of Clinical Laboratory, Wuxi People's Hospital Affiliated to Nanjing 
      Medical University, Wuxi, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200725
PL  - England
TA  - IUBMB Life
JT  - IUBMB life
JID - 100888706
RN  - 0 (Allergens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Peptide Fragments)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/blood/*immunology
MH  - Animals
MH  - Antigens, Dermatophagoides/blood/*immunology
MH  - Arthropod Proteins/immunology
MH  - Asthma/blood/immunology
MH  - Child
MH  - Child, Preschool
MH  - Epitopes/blood/*immunology
MH  - Female
MH  - Humans
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/metabolism
MH  - Immunoglobulin G/blood/*immunology
MH  - Infant
MH  - Male
MH  - Peptide Fragments/*immunology
MH  - Pyroglyphidae/*immunology
OTO - NOTNLM
OT  - IgG
OT  - epitope
OT  - house dust mites
OT  - peptide array
OT  - protein tertiary structure
EDAT- 2020/07/28 06:00
MHDA- 2021/11/23 06:00
CRDT- 2020/07/26 06:00
PHST- 2020/04/09 00:00 [received]
PHST- 2020/05/19 00:00 [revised]
PHST- 2020/05/27 00:00 [accepted]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2021/11/23 06:00 [medline]
PHST- 2020/07/26 06:00 [entrez]
AID - 10.1002/iub.2333 [doi]
PST - ppublish
SO  - IUBMB Life. 2020 Sep;72(9):1976-1985. doi: 10.1002/iub.2333. Epub 2020 Jul 25.