PMID- 32711591
OWN - NLM
STAT- MEDLINE
DCOM- 20210603
LR  - 20220531
IS  - 1533-4899 (Electronic)
IS  - 1533-4880 (Linking)
VI  - 20
IP  - 12
DP  - 2020 Dec 1
TI  - Mind Bomb 1 Promotes Pancreatic Cancer Proliferation by Activating beta-Catenin 
      Signaling.
PG  - 7276-7282
LID - 10.1166/jnn.2020.18755 [doi]
AB  - Mind bomb 1 (MIB1), an E3 ligase, plays a vital role in chemo-resistance and 
      cancer metastasis. According to The Cancer Genome Atlas (TCGA), MIB1 gene is 
      preferentially amplified in pancreatic cancer. Copy number alterations in MIB1 
      gene are associated with worse survival. Gene Expression Omnibus (GEO) also 
      showed that pancreatic cancer with high mRNA level of MIB1 tend to be more 
      resistant to gemcitabine and higher mRNA levels of MIB1 are found in pancreatic 
      tumors compared with adjacent normal tissues. MIB1 knockdown (KD) in Panc-1 and 
      HPAF2 cell lines significantly inhibit proliferation and colony formation of 
      pancreatic cancer. The gene set enrichment analysis (GSEA) has also showed that 
      beta-catenin is the downstream of MIB1. Western blot analysis showed that total and 
      active beta-catenin levels are decreased in MIB1 KD cells. beta-catenin inhibitor also 
      inhibits proliferation of Panc-1 and HPAF2 cells. We in this study implanted 
      HPAF2 scramble and MIB1 KD cells orthotopically in athymic nude mice. Gemcitabine 
      was used to treat the mice. Results revealed that after MIB1 KD HPAF2 cells were 
      more sensitive to gemcitabine. In conclusion, we demonstrated that MIB1 promotes 
      pancreatic cancer proliferation through activating beta-catenin signaling. MIB1 may 
      thus be a therapeutic target in pancreatic cancer therapy.
FAU - Fu, Xiao
AU  - Fu X
AD  - Department of General Surgery, The Affiliated Drum Tower Hospital of Nanjing 
      University Medical School, Nanjing, 210008, China.
FAU - Tang, Neng
AU  - Tang N
AD  - Department of General Surgery, The Affiliated Drum Tower Hospital of Nanjing 
      University Medical School, Nanjing, 210008, China.
FAU - Xie, Weiqi
AU  - Xie W
AD  - Department of General Surgery, The Affiliated Drum Tower Hospital of Nanjing 
      University Medical School, Nanjing, 210008, China.
FAU - Mao, Liang
AU  - Mao L
AD  - Department of General Surgery, The Affiliated Drum Tower Hospital of Nanjing 
      University Medical School, Nanjing, 210008, China.
FAU - Qiu, Yudong
AU  - Qiu Y
AD  - Department of General Surgery, The Affiliated Drum Tower Hospital of Nanjing 
      University Medical School, Nanjing, 210008, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nanosci Nanotechnol
JT  - Journal of nanoscience and nanotechnology
JID - 101088195
RN  - 0 (beta Catenin)
RN  - EC 2.3.2.27 (MIB1 ligase, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Mice
MH  - Mice, Nude
MH  - *Pancreatic Neoplasms/drug therapy/genetics
MH  - *Signal Transduction
MH  - *Ubiquitin-Protein Ligases
MH  - *beta Catenin/genetics/metabolism
EDAT- 2020/07/28 06:00
MHDA- 2021/06/04 06:00
CRDT- 2020/07/27 06:00
PHST- 2020/07/27 06:00 [entrez]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2021/06/04 06:00 [medline]
AID - 10.1166/jnn.2020.18755 [doi]
PST - ppublish
SO  - J Nanosci Nanotechnol. 2020 Dec 1;20(12):7276-7282. doi: 10.1166/jnn.2020.18755.