PMID- 32712772
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1939-8425 (Print)
IS  - 1939-8433 (Electronic)
IS  - 1939-8425 (Linking)
VI  - 13
IP  - 1
DP  - 2020 Jul 25
TI  - Disruption of a Rice Chloroplast-Targeted Gene OsHMBPP Causes a Seedling-Lethal 
      Albino Phenotype.
PG  - 51
LID - 10.1186/s12284-020-00408-1 [doi]
LID - 51
AB  - BACKGROUND: Chloroplast development is coordinately regulated by plastid- and 
      nuclear-encoding genes. Although many regulators have been reported to be 
      involved in chloroplast development, new factors remain to be identified, given 
      the complexity of this process. RESULTS: In this study, we characterized a rice 
      mutant lethal albinic seedling 1(las1)form of a 4-hydroxy-3-methylbut-2-enyl 
      diphosphate reductase (OsHMBPP) that was targeted to the chloroplasts. The LAS1 
      mutation caused the albino lethal phenotype in seedlings. Transmission electron 
      microscopy indicated that las1 were defective in early chloroplast development. 
      LAS1 is preferentially expressed in leaves, implying its role in controlling 
      chloroplast development. The expression levels of many chloroplast-encoded genes 
      were altered significantly in las1. The expression levels of nuclear-encoded gene 
      involved in Chl biosynthesis were also decreased in las1. We further investigated 
      plastidic RNA editing in las1 and found that the edit efficiency of four 
      chloroplast genes were markly altered. Compared with WT, las1 exhibited defective 
      in biogenesis of chloroplast ribosomes. CONCLUSIONS: Our results show that 
      LAS1/OsHMBPP plays an essential role in the early chloroplast development in 
      rice.
FAU - Liu, X
AU  - Liu X
AUID- ORCID: 0000-0002-1901-1506
AD  - Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake, Regional 
      Cooperative Innovation Center for Modern Agriculture and Environmental 
      Protection, Huaiyin Normal University, Huaian, 223300, China. 1240623244@qq.com.
FAU - Cao, P H
AU  - Cao PH
AD  - Suzhou Academy of Agricultural Sciences, Suzhou, 215155, China.
FAU - Huang, Q Q
AU  - Huang QQ
AD  - Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake, Regional 
      Cooperative Innovation Center for Modern Agriculture and Environmental 
      Protection, Huaiyin Normal University, Huaian, 223300, China.
FAU - Yang, Y R
AU  - Yang YR
AD  - Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake, Regional 
      Cooperative Innovation Center for Modern Agriculture and Environmental 
      Protection, Huaiyin Normal University, Huaian, 223300, China.
FAU - Tao, D D
AU  - Tao DD
AD  - Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake, Regional 
      Cooperative Innovation Center for Modern Agriculture and Environmental 
      Protection, Huaiyin Normal University, Huaian, 223300, China.
LA  - eng
GR  - 31900388/National Natural Science Foundation of China/
GR  - BK20191055/Natural Science Foundation of Jiangsu Province/
GR  - 19KJB180009/Natural Science Foundation of Colleges of Jiangsu Province/
PT  - Journal Article
DEP - 20200725
PL  - United States
TA  - Rice (N Y)
JT  - Rice (New York, N.Y.)
JID - 101503136
PMC - PMC7382669
OTO - NOTNLM
OT  - Albino lethal
OT  - CRISPR/Cas9
OT  - Chloroplast development
OT  - RNA editing
OT  - Rice (Oryza sativa L.)
COIS- The authors declare that they have no competing interests.
EDAT- 2020/07/28 06:00
MHDA- 2020/07/28 06:01
PMCR- 2020/07/25
CRDT- 2020/07/27 06:00
PHST- 2020/01/20 00:00 [received]
PHST- 2020/07/06 00:00 [accepted]
PHST- 2020/07/27 06:00 [entrez]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2020/07/28 06:01 [medline]
PHST- 2020/07/25 00:00 [pmc-release]
AID - 10.1186/s12284-020-00408-1 [pii]
AID - 408 [pii]
AID - 10.1186/s12284-020-00408-1 [doi]
PST - epublish
SO  - Rice (N Y). 2020 Jul 25;13(1):51. doi: 10.1186/s12284-020-00408-1.