PMID- 32713334
OWN - NLM
STAT- MEDLINE
DCOM- 20211223
LR  - 20211223
IS  - 1875-5666 (Electronic)
IS  - 1566-5240 (Linking)
VI  - 21
IP  - 3
DP  - 2021
TI  - The Melatonin and Enriched Environment Ameliorated Low Protein-Induced 
      Intrauterine Growth Retardation by IGF-1 And mtor Signaling Pathway and Autophagy 
      Inhibition in Rats.
PG  - 246-256
LID - 10.2174/1566524020666200726221735 [doi]
AB  - CDATA[Aim: The present study investigated whether melatonin (MEL) and enriched 
      environment (EE) might protect against intrauterine growth retardation (IUGR) in 
      rats. METHODS: Sprague-Dawley rats were randomly allocated to 3 groups: control 
      (C), model (M) and EE+MEL group. Animals were housed in an enriched environment 
      (EE+MEL group) or remained in a standard environment (C group, M group). IUGR rat 
      model was built by feeding a low protein diet during pregnancy. MEL was 
      administered by gavaging. At day 1 post-birth, the baseline characteristics and 
      serum biochemical parameters, morphology of liver and small intestine, enzyme 
      activities, and mRNA expression levels of fetal rats were determined. The 
      autophagy marker LC3 and Beclin1 were determined by western blot analysis. 
      RESULTS: EE+MEL markedly improved the baseline characteristics, hepatic and 
      intestinal morphology of IUGR fetuses. In addition, the lactase activities in the 
      fetal intestine were markedly increased by the EE+MEL. The levels of serum 
      somatostatin (SST), Growth hormone (GH), GH releasing hormone (GHRH), 
      Insulin-like Growth Factor 1 (IGF-1), triiodothyronine (T3), and 
      tetraiodothyronine (T4) were found to be recovered by EE+MEL. In addition, the 
      EE+MEL significantly ameliorated the mRNA expression of SST, GHRH, and GHRH 
      receptor (GHRHR), GH, GHR, IGF-1, and IGF-1 receptor (IGF1R), IGF binding 
      protein-1 (IGFBP1), mammalian target of rapamycin (mTOR), S6 kinase 1 (S6K1) and 
      eukaryotic initiation factor 4E (eIF4E)-binding protein 1 (4EBP1) in fetuses. In 
      IUGR fetal livers, LC3 and Beclin1 were found to be increased at birth, while LC3 
      and Beclin1 were observed to be significantly decreased in the EE+MEL group. 
      CONCLUSION: EE+MEL could improve fetal rats' baseline characteristics, serum 
      biochemical parameters, birth weight, intestinal and hepatic morphology and 
      enzyme activities. These effects could be explained by the activation of the 
      IGF-1/IGFBP1 and IGF-1/mTOR/S6K1/4EBP1 signaling pathway and autophagy 
      inhibition.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Wang, Dan
AU  - Wang D
AD  - College of Human Kinesiology, Shenyang Sport University, 36 Jinqiansong East Road 
      Sujiatun District, Shenyang, 110102, Liaoning, China.
FAU - Wu, Xiao
AU  - Wu X
AD  - Department of basic medical, HE's University, Shenyang, Liaoning 110163, China.
FAU - Lu, Dan
AU  - Lu D
AD  - College of clinical, HE's University, Shenyang, Liaoning 110163, China.
FAU - Li, Yan
AU  - Li Y
AD  - Experimental Teaching Center of Pharmacology, School of Life Science and 
      Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang , Liaoning 110016, 
      China.
FAU - Zhang, Peng
AU  - Zhang P
AD  - Department of basic medical, HE's University, Shenyang, Liaoning 110163, China.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Curr Mol Med
JT  - Current molecular medicine
JID - 101093076
RN  - 0 (Insulin-Like Growth Factor Binding Protein 1)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9002-72-6 (Growth Hormone)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Autophagy/drug effects/genetics
MH  - Diet, Protein-Restricted/adverse effects
MH  - Disease Models, Animal
MH  - Female
MH  - Fetal Growth Retardation/*drug therapy/genetics/metabolism/pathology
MH  - Gene Expression Regulation/drug effects
MH  - Growth Hormone/genetics
MH  - Insulin-Like Growth Factor Binding Protein 1/genetics
MH  - Insulin-Like Growth Factor I/*genetics
MH  - Male
MH  - Melatonin/*pharmacology
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/*genetics
OTO - NOTNLM
OT  - Autophagy
OT  - Intrauterine growth restriction
OT  - Melatonin
OT  - enriched environment
OT  - insulin-like growth factor-1
OT  - mTOR
EDAT- 2020/07/28 06:00
MHDA- 2021/12/24 06:00
CRDT- 2020/07/28 06:00
PHST- 2020/03/10 00:00 [received]
PHST- 2020/06/26 00:00 [revised]
PHST- 2020/06/28 00:00 [accepted]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2021/12/24 06:00 [medline]
PHST- 2020/07/28 06:00 [entrez]
AID - CMM-EPUB-108465 [pii]
AID - 10.2174/1566524020666200726221735 [doi]
PST - ppublish
SO  - Curr Mol Med. 2021;21(3):246-256. doi: 10.2174/1566524020666200726221735.