PMID- 32714196
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220415
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 11
DP  - 2020
TI  - Human Umbilical Cord Mesenchymal Stem Cell Differentiation Into Odontoblast-Like 
      Cells and Endothelial Cells: A Potential Cell Source for Dental Pulp Tissue 
      Engineering.
PG  - 593
LID - 10.3389/fphys.2020.00593 [doi]
LID - 593
AB  - OBJECTIVES: Dental pulp regeneration is considered an ideal approach for treating 
      dental pulp disease. Because pulp is composed of various cells, determining the 
      proper seed cells is critical. We explored the potential of human umbilical cord 
      mesenchymal stem cells (hUCMSCs) as seed cells for dental pulp regeneration. 
      METHODS: Liquid extract of human treated dentin matrix (LE-TDM) was acquired to 
      culture hUCMSCs. Odontoblast-specific markers were detected by western blot, 
      qRT-PCR, and immunofluorescence assays. Endothelial differentiation of hUCMSCs 
      was examined according to VEGF induction by western blot, qRT-PCR, and Matrigel 
      assays. hUCMSCs and VEGF-induced hUCMSCs (V-hUCMSCs) were also cocultured in vivo 
      for the Matrigel plug assay and in vitro for RNA-sequencing (RNA-seq). Finally, 
      encapsulated mono-cultured hUCMSCs or cocultured hUCMSCs and V-hUCMSCs in 
      scaffolds were injected into the root segments and transplanted into 
      immunodeficient mice for dental pulp regeneration. RESULTS: Under LE-TDM 
      induction, hUCMSCs expressed specific odontoblast markers (DSPP, DMP-1, DSP). 
      Under VEGF induction, hUCMSCs expressed functional endothelial markers (CD31, 
      eNOs, vWF). In vivo, the Matrigel plug assay indicated that cocultured hUCMSCs 
      and V-hUCMSCs formed extensive vessel-like structures. RNA-seq results indicated 
      that cocultured V-hUCMSCs exhibited high Hif-1 signaling pathway activity. Both 
      the hUCMSCs mono-culture and coculture groups showed pulp-like tissue 
      regeneration. The cocultured group showed more extracellular matrix and 
      vascularization than the mono-cultured group in vivo. CONCLUSION: hUCMSCs can 
      differentiate into odontoblast-like cells and functional endothelial cells. 
      Cocultured hUCMSCs and V-hUCMSCs formed vessel-like structures and regenerated 
      dental pulp-like tissue. Therefore, hUCMSCs can be used as an alternative seed 
      cell source for angiogenesis and dental pulp regeneration.
CI  - Copyright (c) 2020 Zhang, Zhang, Li, Ren, Deng, Yin, Gao, Pan and Niu.
FAU - Zhang, Shuang
AU  - Zhang S
AD  - Department of Endodontics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
AD  - Department of Endodontics, School of Stomatology, Harbin Medical University, 
      Harbin, China.
FAU - Zhang, Weiwei
AU  - Zhang W
AD  - Department of Endodontics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
AD  - Department of Endodontics, School of Stomatology, Harbin Medical University, 
      Harbin, China.
FAU - Li, Yanping
AU  - Li Y
AD  - Department of Endodontics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
AD  - Department of Endodontics, School of Stomatology, Harbin Medical University, 
      Harbin, China.
FAU - Ren, Liping
AU  - Ren L
AD  - Department of Prosthodontics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Deng, Haotian
AU  - Deng H
AD  - Department of Endodontics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
AD  - Department of Endodontics, School of Stomatology, Harbin Medical University, 
      Harbin, China.
FAU - Yin, Xiaowei
AU  - Yin X
AD  - Department of Endodontics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
AD  - Department of Endodontics, School of Stomatology, Harbin Medical University, 
      Harbin, China.
FAU - Gao, Xu
AU  - Gao X
AD  - Department of Biochemistry and Molecular Biology, Harbin Medical University, 
      Harbin, China.
FAU - Pan, Shuang
AU  - Pan S
AD  - Department of Endodontics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
AD  - Department of Endodontics, School of Stomatology, Harbin Medical University, 
      Harbin, China.
FAU - Niu, Yumei
AU  - Niu Y
AD  - Department of Endodontics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
AD  - Department of Endodontics, School of Stomatology, Harbin Medical University, 
      Harbin, China.
LA  - eng
PT  - Journal Article
DEP - 20200623
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC7344301
OTO - NOTNLM
OT  - Hif-1 signaling pathway
OT  - angiogenesis
OT  - dental pulp tissue engineering
OT  - endothelial differentiation
OT  - human umbilical cord mesenchymal stem cells
OT  - odontoblast-like cells
EDAT- 2020/07/28 06:00
MHDA- 2020/07/28 06:01
PMCR- 2020/06/23
CRDT- 2020/07/28 06:00
PHST- 2020/02/05 00:00 [received]
PHST- 2020/05/11 00:00 [accepted]
PHST- 2020/07/28 06:00 [entrez]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2020/07/28 06:01 [medline]
PHST- 2020/06/23 00:00 [pmc-release]
AID - 10.3389/fphys.2020.00593 [doi]
PST - epublish
SO  - Front Physiol. 2020 Jun 23;11:593. doi: 10.3389/fphys.2020.00593. eCollection 
      2020.