PMID- 32714396
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220415
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2020
DP  - 2020
TI  - Cerebrospinal Fluid Pulsation Stress Promotes the Angiogenesis of 
      Tissue-Engineered Laminae.
PG  - 8026362
LID - 10.1155/2020/8026362 [doi]
LID - 8026362
AB  - BACKGROUND: Angiogenesis is a prerequisite step to achieve the success of bone 
      regeneration by tissue engineering technology. Previous studies have shown the 
      role of cerebrospinal fluid pulsation (CSFP) stress in the reconstruction of 
      tissue-engineered laminae. In this study, we investigated the role of CSFP stress 
      in the angiogenesis of tissue-engineered laminae. METHODS: For the in vitro 
      study, a CSFP bioreactor was used to investigate the impact of CSFP stress on the 
      osteogenic mesenchymal stem cells (MSCs). For the in vivo study, forty-eight New 
      Zealand rabbits were randomly divided into the CSFP group and the Non-CSFP group. 
      Tissue-engineered laminae (TEL) was made by hydroxyapatite-collagen I scaffold 
      and osteogenic MSCs and then implanted into the lamina defect in the two groups. 
      The angiogenic and osteogenic abilities of newborn laminae were examined with 
      histological staining, qRT-PCR, and radiological analysis. RESULTS: The in vitro 
      study showed that CSFP stress could promote the vascular endothelial growth 
      factor A (VEGF-A) expression levels of osteogenic MSCs. In the animal study, the 
      expression levels of angiogenic markers in the CSFP group were higher than those 
      in the Non-CSFP group; moreover, in the CSFP group, their expression levels on 
      the dura mater surface, which are closer to the CSFP stress stimulation, were 
      also higher than those on the paraspinal muscle surface. The expression levels of 
      osteogenic markers in the CSFP group were also higher than those in the Non-CSFP 
      group. CONCLUSION: CSFP stress could promote the angiogenic ability of osteogenic 
      MSCs and thus promote the angiogenesis of tissue-engineered laminae. The 
      pretreatment of osteogenic MSC with a CSFP bioreactor may have important 
      implications for vertebral lamina reconstruction with a tissue engineering 
      technique.
CI  - Copyright (c) 2020 Linli Li et al.
FAU - Li, Linli
AU  - Li L
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      China.
FAU - He, Yiqun
AU  - He Y
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      China.
FAU - Tang, Han
AU  - Tang H
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      China.
FAU - Mao, Wei
AU  - Mao W
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      China.
FAU - Ni, Haofei
AU  - Ni H
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      China.
FAU - Lyu, Feizhou
AU  - Lyu F
AUID- ORCID: 0000-0001-8887-5554
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      China.
AD  - Department of Orthopedics, Huashan Hospital, Fudan University, China.
FAU - Dong, Youhai
AU  - Dong Y
AUID- ORCID: 0000-0002-3891-9093
AD  - Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20200702
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC7352145
COIS- The authors declare that there are no conflicts of interest regarding the 
      publication of this paper.
EDAT- 2020/07/28 06:00
MHDA- 2020/07/28 06:01
PMCR- 2020/07/02
CRDT- 2020/07/28 06:00
PHST- 2020/03/02 00:00 [received]
PHST- 2020/05/11 00:00 [revised]
PHST- 2020/05/17 00:00 [accepted]
PHST- 2020/07/28 06:00 [entrez]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2020/07/28 06:01 [medline]
PHST- 2020/07/02 00:00 [pmc-release]
AID - 10.1155/2020/8026362 [doi]
PST - epublish
SO  - Stem Cells Int. 2020 Jul 2;2020:8026362. doi: 10.1155/2020/8026362. eCollection 
      2020.