PMID- 3271462
OWN - NLM
STAT- MEDLINE
DCOM- 19900320
LR  - 20131121
IS  - 0739-1102 (Print)
IS  - 0739-1102 (Linking)
VI  - 5
IP  - 1
DP  - 1987 Aug
TI  - Interactions of acridine orange with double stranded nucleic acids. Spectral and 
      affinity studies.
PG  - 127-43
AB  - Spectral properties of acridine orange (AO) alone or in complexes with natural 
      and synthetic nucleic acids of various base composition have been studied in 
      aqueous solutions by absorption and fluorescence spectroscopy. The dimerization 
      constant and absorption spectra of the dye in monomeric and dimeric form were 
      established; dimerization of AO resulted in quenching of its fluorescence. 
      Complexes of the dye with synthetic nucleic acids differed in the degree of 
      enhancement of fluorescence quantum yield, varying between 1.42 to 2.38 fold as 
      compared to AO monomer; these differences, however, were not base-dependent. 
      Affinity of the dye to natural and synthetic polymers was studied and analyzed 
      using McGhee-von Hippel model of polymer-ligand interactions. Because the 
      sterical requirement for intercalative binding assumes interaction of dye 
      monomer, the correction for AO dimerization was made in all calculations. All 
      studied DNAs (natural and synthetic ones, the latter being homopolymer pairs or 
      alternating copolymers of A,T or G,C or I,C base composition) had similar 
      intrinsic association constants (KI = 5 X 10(4) - 1 X 10(5), M-1) and binding 
      site size (n = 2.0-2.4 b.p.). The exception was poly(dA).poly(dT), having KI = 
      1.2 X 10(4) and n = 19.3 b.p. The results of KI measurement for calf thymus DNA 
      and AO in different sodium ion concentration were in good agreement with 
      predictions of the counterion condensation theory. The intercalation of AO into 
      DNA is discussed in view of recent theoretical models of DNA-ligand interactions.
FAU - Kapuscinski, J
AU  - Kapuscinski J
AD  - Sloan-Kettering Institute, Cancer Research Walker Laboratory, Rye, N.Y. 10580.
FAU - Darzynkiewicz, Z
AU  - Darzynkiewicz Z
LA  - eng
GR  - CA 23296/CA/NCI NIH HHS/United States
GR  - CA 28704/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Biomol Struct Dyn
JT  - Journal of biomolecular structure & dynamics
JID - 8404176
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Intercalating Agents)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*metabolism/pharmacology
MH  - DNA/drug effects/*metabolism/ultrastructure
MH  - Fluorescent Dyes/*metabolism/pharmacology
MH  - Intercalating Agents/*metabolism/pharmacology
MH  - Nucleic Acid Conformation/drug effects
MH  - Oligodeoxyribonucleotides/metabolism
MH  - Spectrometry, Fluorescence
EDAT- 1987/08/01 00:00
MHDA- 1987/08/01 00:01
CRDT- 1987/08/01 00:00
PHST- 1987/08/01 00:00 [pubmed]
PHST- 1987/08/01 00:01 [medline]
PHST- 1987/08/01 00:00 [entrez]
AID - 10.1080/07391102.1987.10506381 [doi]
PST - ppublish
SO  - J Biomol Struct Dyn. 1987 Aug;5(1):127-43. doi: 10.1080/07391102.1987.10506381.