PMID- 32715402
OWN - NLM
STAT- MEDLINE
DCOM- 20220113
LR  - 20240226
IS  - 1573-6830 (Electronic)
IS  - 0272-4340 (Linking)
VI  - 41
IP  - 7
DP  - 2021 Oct
TI  - HBO-PC Promotes Locomotor Recovery by Reducing Apoptosis and Inflammation in SCI 
      Rats: The Role of the mTOR Signaling Pathway.
PG  - 1537-1547
LID - 10.1007/s10571-020-00921-3 [doi]
AB  - Hyperbaric oxygen preconditioning (HBO-PC) has beneficial effects on the 
      promotion of locomotor recovery by reducing apoptosis and inflammation after 
      traumatic spinal cord injury (SCI). The mammalian target of rapamycin (mTOR) 
      signaling pathway has been implicated in apoptosis and inflammation in many 
      pathophysiological conditions. However, whether HBO-PC improves traumatic 
      SCI-induced locomotor dysfunction by regulating the mTOR signaling pathway and 
      its downstream molecules remains unknown. In the present study, we found that 
      HBO-PC significantly promoted SCI-induced hind-limb locomotor recovery and 
      increased the amplitude and potential of motor evoked potential. Magnetic 
      resonance imaging showed that spinal cavitation or atrophy caused by SCI was 
      obviously alleviated by HBO-PC therapy. Histological analysis showed that the 
      changes in spinal cord neural structure in SCI rats were markedly restored by 
      HBO-PC treatment. Western blot analysis showed that the SCI-induced enhanced 
      levels of p-mTOR, inflammatory cytokines and apoptosis in the spinal cord were 
      abrogated after administration of HBO-PC. Furthermore, intrathecal administration 
      of an mTOR agonist reversed the effects of HBO-PC on locomotor function recovery, 
      p-NF-kappaB p65 and p-p70S6K levels, inflammation and apoptosis. These findings 
      indicated a new mechanism by which HBO-PC therapy suppressed inflammation and 
      apoptosis through inactivation of the mTOR signaling pathway, which contributed 
      to motor disability in SCI rats.
CI  - (c) 2020. Springer Science+Business Media, LLC, part of Springer Nature.
FAU - Chen, Huiqiang
AU  - Chen H
AUID- ORCID: 0000-0001-8573-3921
AD  - Department of Orthopedics, General Hospital of Southern Theater Command, 
      Gusangzhou, 510010, Guangdong Province, China.
FAU - Xu, Guoshen
AU  - Xu G
AUID- ORCID: 0000-0001-7683-9724
AD  - Department of Orthopedics, Guangdong Tongjiang Hospital, Foshan, 528300, 
      Guangdong Province, China.
FAU - Wu, You
AU  - Wu Y
AUID- ORCID: 0000-0003-1533-5551
AD  - Department of Orthopedics, General Hospital of Southern Theater Command, 
      Gusangzhou, 510010, Guangdong Province, China.
FAU - Wang, Xinyu
AU  - Wang X
AUID- ORCID: 0000-0001-8087-1022
AD  - Department of Orthopedics, General Hospital of Southern Theater Command, 
      Gusangzhou, 510010, Guangdong Province, China.
FAU - Wang, Fei
AU  - Wang F
AUID- ORCID: 0000-0003-1175-3166
AD  - Department of Orthopedics, General Hospital of Southern Theater Command, 
      Gusangzhou, 510010, Guangdong Province, China.
FAU - Zhang, Ying
AU  - Zhang Y
AUID- ORCID: 0000-0001-8159-6069
AD  - Department of Orthopedics, General Hospital of Southern Theater Command, 
      Gusangzhou, 510010, Guangdong Province, China. zhangying_doc@aliyun.com.
LA  - eng
GR  - 2017A030313693/Natural Science Foundation of Guangdong Province/
GR  - CGZ16C004/the Research Program of PLA)/
GR  - CWH17J022/Military Logistics Research Project/
PT  - Journal Article
DEP - 20200726
PL  - United States
TA  - Cell Mol Neurobiol
JT  - Cellular and molecular neurobiology
JID - 8200709
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/*physiology
MH  - Inflammation/*metabolism
MH  - Locomotion/physiology
MH  - Male
MH  - Motor Activity/physiology
MH  - Neurons/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/physiology
MH  - Signal Transduction/physiology
MH  - Spinal Cord Injuries/*metabolism/pathology/physiopathology
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Rats
OTO - NOTNLM
OT  - Apoptosis
OT  - HBO-PC
OT  - Inflammation
OT  - Spinal cord injury
OT  - mTOR
EDAT- 2020/07/28 06:00
MHDA- 2022/01/14 06:00
CRDT- 2020/07/28 06:00
PHST- 2019/10/31 00:00 [received]
PHST- 2020/07/10 00:00 [accepted]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2022/01/14 06:00 [medline]
PHST- 2020/07/28 06:00 [entrez]
AID - 10.1007/s10571-020-00921-3 [pii]
AID - 10.1007/s10571-020-00921-3 [doi]
PST - ppublish
SO  - Cell Mol Neurobiol. 2021 Oct;41(7):1537-1547. doi: 10.1007/s10571-020-00921-3. 
      Epub 2020 Jul 26.