PMID- 32716099
OWN - NLM
STAT- MEDLINE
DCOM- 20210623
LR  - 20210623
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 44
IP  - 11
DP  - 2020 Nov
TI  - Interleukin-38 inhibits adipogenesis and inflammatory cytokine production in 
      3T3-L1 preadipocytes.
PG  - 2357-2362
LID - 10.1002/cbin.11428 [doi]
AB  - Interleukin-38 (IL-38) is a novel member of the IL-1 cytokine family with 
      anti-inflammatory activity. However, its effect on adipogenesis and inflammatory 
      cytokines secretion of adipocytes in vitro has not been reported. To address 
      whether IL-38 inhibits adipogenesis and inflammation in vitro, adipose precursor 
      3T3-L1 cells were cultured with or without IL-38. The morphology and size of 
      lipid droplets in 3T3-L1 cells were measured by Oil red O staining. The mRNA 
      expression levels of GATA-binding protein-3 (GATA-3), glucose transporter type 4 
      (GLUT4), peroxisome proliferator-associated receptor gamma2, IL-1beta, IL-6, and 
      monocyte chemoattractant protein-1 (MCP-1) in 3T3-L1 cells were detected by 
      real-time PCR, The contents of IL-6, IL-1beta, and MCP-1 in 3T3-L1 cell medium 
      supernatants were determined by enzyme-linked immunosorbent assay. IL-38 
      significantly decreased the number of lipid droplets in 3T3-L1 cells. IL-38 also 
      increased GATA-3 and GLUT4 mRNA expression and inhibited IL-1beta, IL-6, and MCP-1 
      secretion by 3T3-L1 cells. It is concluded that IL-38 can inhibit the 
      differentiation of human adipocytes and inflammatory cytokine production by 
      3T3-L1 cells.
CI  - (c) 2020 International Federation for Cell Biology.
FAU - Li, Yan
AU  - Li Y
AD  - Department of Immunology, Ningbo University School of Medicine, Ningbo, China.
FAU - Chen, Sisi
AU  - Chen S
AD  - Department of Immunology, Ningbo University School of Medicine, Ningbo, China.
FAU - Sun, Jun
AU  - Sun J
AD  - Department of Immunology, Ningbo University School of Medicine, Ningbo, China.
FAU - Yu, Yang
AU  - Yu Y
AD  - The Fifth People's Hospital of Jilin City, Jilin, China.
FAU - Li, Mingcai
AU  - Li M
AUID- ORCID: 0000-0003-3910-6964
AD  - Department of Immunology, Ningbo University School of Medicine, Ningbo, China.
LA  - eng
PT  - Journal Article
DEP - 20200731
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Il1f10 protein, mouse)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukins)
SB  - IM
MH  - 3T3-L1 Cells/metabolism
MH  - Adipocytes/*metabolism
MH  - Adipogenesis/*genetics
MH  - Adipose Tissue/metabolism
MH  - Animals
MH  - Anti-Inflammatory Agents/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cytokines/metabolism
MH  - Inflammation/metabolism
MH  - Interleukin-1/genetics/*metabolism
MH  - Interleukins/metabolism
MH  - Lipid Metabolism/drug effects
MH  - Mice
OTO - NOTNLM
OT  - adipocyte
OT  - anti-inflammation
OT  - differentiation
OT  - interleukin-38
OT  - lipogenesis
EDAT- 2020/07/28 06:00
MHDA- 2021/06/24 06:00
CRDT- 2020/07/28 06:00
PHST- 2020/04/29 00:00 [received]
PHST- 2020/06/25 00:00 [revised]
PHST- 2020/07/23 00:00 [accepted]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2021/06/24 06:00 [medline]
PHST- 2020/07/28 06:00 [entrez]
AID - 10.1002/cbin.11428 [doi]
PST - ppublish
SO  - Cell Biol Int. 2020 Nov;44(11):2357-2362. doi: 10.1002/cbin.11428. Epub 2020 Jul 
      31.