PMID- 32717403
OWN - NLM
STAT- MEDLINE
DCOM- 20210427
LR  - 20211002
IS  - 1090-2139 (Electronic)
IS  - 0889-1591 (Print)
IS  - 0889-1591 (Linking)
VI  - 89
DP  - 2020 Oct
TI  - Expression of a heroin contextually conditioned immune effect in male rats 
      requires CaMKIIalpha-expressing neurons in dorsal, but not ventral, subiculum and 
      hippocampal CA1.
PG  - 414-422
LID - S0889-1591(20)30917-X [pii]
LID - 10.1016/j.bbi.2020.07.028 [doi]
AB  - The physiological and motivational effects of heroin and other abused drugs 
      become associated with environmental (contextual) stimuli during repeated drug 
      use. As a result, these contextual stimuli gain the ability to elicit drug-like 
      conditioned effects. For example, after context-heroin pairings, exposure to the 
      heroin-paired context alone produces similar effects on peripheral immune 
      function as heroin itself. Conditioned immune effects can significantly 
      exacerbate the adverse health consequences of heroin use. Our laboratory has 
      shown that exposure to a heroin-paired context suppresses lipopolysaccharide 
      (LPS)-induced splenic nitric oxide (NO) production in male rats, and this effect 
      is mediated in part by the dorsal hippocampus (dHpc). However, specific dHpc 
      output regions, whose efferents might mediate conditioned immune effects, have 
      not been identified, nor has the contribution of ventral hippocampus (vHpc) been 
      investigated. Here, we evaluated the role of CaMKIIalpha-expressing neurons in the 
      dHpc and vHpc main output regions by expressing G(i)-coupled designer receptors 
      exclusively activated by designer drugs (DREADDs) under a CaMKIIalpha promoter in the 
      dorsal subiculum and CA1 (dSub, dCA1) or ventral subiculum and CA1 (vSub, vCA1). 
      After context-heroin conditioning, clozapine-N-oxide (CNO, DREADD agonist) or 
      vehicle was administered systemically prior to heroin-paired context (or 
      home-cage control) exposure and LPS immune challenge. Chemogenetic inhibition of 
      CaMKIIalpha-expressing neurons in dHpc, but not vHpc, output regions attenuated the 
      expression of conditioned splenic NO suppression. These results establish that 
      the main dHpc output regions, the dSub and dCA1, are critical for this 
      context-heroin conditioned immune effect.
CI  - Published by Elsevier Inc.
FAU - Lebonville, Christina L
AU  - Lebonville CL
AD  - Department of Psychology and Neuroscience, University of North Carolina at Chapel 
      Hill, CB#3270, Chapel Hill, NC 27599-3270 USA.
FAU - Paniccia, Jacqueline E
AU  - Paniccia JE
AD  - Department of Psychology and Neuroscience, University of North Carolina at Chapel 
      Hill, CB#3270, Chapel Hill, NC 27599-3270 USA.
FAU - Parekh, Shveta V
AU  - Parekh SV
AD  - Department of Psychology and Neuroscience, University of North Carolina at Chapel 
      Hill, CB#3270, Chapel Hill, NC 27599-3270 USA.
FAU - Wangler, Lynde M
AU  - Wangler LM
AD  - Department of Psychology and Neuroscience, University of North Carolina at Chapel 
      Hill, CB#3270, Chapel Hill, NC 27599-3270 USA.
FAU - Jones, Meghan E
AU  - Jones ME
AD  - Department of Psychology and Neuroscience, University of North Carolina at Chapel 
      Hill, CB#3270, Chapel Hill, NC 27599-3270 USA.
FAU - Fuchs, Rita A
AU  - Fuchs RA
AD  - Integrative Physiology and Neuroscience, College of Veterinary Medicine, 
      Washington State University, P.O. Box 647620, Pullman, WA 99164-7620, USA.
FAU - Lysle, Donald T
AU  - Lysle DT
AD  - Department of Psychology and Neuroscience, University of North Carolina at Chapel 
      Hill, CB#3270, Chapel Hill, NC 27599-3270 USA. Electronic address: 
      dlysle@email.unc.edu.
LA  - eng
GR  - F31 DA047054/DA/NIDA NIH HHS/United States
GR  - R01 DA025646/DA/NIDA NIH HHS/United States
GR  - R01 DA034721/DA/NIDA NIH HHS/United States
GR  - T32 DA007244/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20200724
PL  - Netherlands
TA  - Brain Behav Immun
JT  - Brain, behavior, and immunity
JID - 8800478
RN  - 70D95007SX (Heroin)
SB  - IM
MH  - Animals
MH  - Conditioning, Classical
MH  - *Heroin
MH  - *Hippocampus
MH  - Male
MH  - Neurons
MH  - Rats
PMC - PMC7572614
MID - NIHMS1618266
OTO - NOTNLM
OT  - Chemogenetics
OT  - DREADD
OT  - Heroin
OT  - Hippocampus
OT  - Immune conditioning
OT  - Learning
OT  - Nitric oxide
OT  - Opioid
EDAT- 2020/07/28 06:00
MHDA- 2021/04/28 06:00
PMCR- 2021/10/01
CRDT- 2020/07/28 06:00
PHST- 2020/05/11 00:00 [received]
PHST- 2020/07/16 00:00 [revised]
PHST- 2020/07/20 00:00 [accepted]
PHST- 2020/07/28 06:00 [pubmed]
PHST- 2021/04/28 06:00 [medline]
PHST- 2020/07/28 06:00 [entrez]
PHST- 2021/10/01 00:00 [pmc-release]
AID - S0889-1591(20)30917-X [pii]
AID - 10.1016/j.bbi.2020.07.028 [doi]
PST - ppublish
SO  - Brain Behav Immun. 2020 Oct;89:414-422. doi: 10.1016/j.bbi.2020.07.028. Epub 2020 
      Jul 24.