PMID- 32718596
OWN - NLM
STAT- MEDLINE
DCOM- 20210414
LR  - 20210414
IS  - 1879-1344 (Electronic)
IS  - 0144-8617 (Linking)
VI  - 245
DP  - 2020 Oct 1
TI  - A new application of acetylation for analysis of acidic heteropolysaccharides by 
      liquid chromatography-electrospray mass spectrometry.
PG  - 116439
LID - S0144-8617(20)30613-5 [pii]
LID - 10.1016/j.carbpol.2020.116439 [doi]
AB  - A novel ultra-high-performance liquid chromatography-electrospray mass 
      spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of 
      neutral and acidic monosaccharaides by employing acetylation derivatization that 
      is specialized for gas chromatography-mass spectrometry (GC-MS) analyses. The 
      UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion 
      monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and 
      Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA). The separation was achieved 
      on a Cortecs C18(+) (2.1 mm x 150 mm, 1.6 mum) within 8 min using acetonitrile 
      water as the mobile phase without additional buffer salt added. Furthermore, 
      quantitative analysis of the multi-monosaccharaides by single marker (QAMS) was 
      developed and validated using a relative correction factor. The UHPLC-ESI-MS and 
      QAMS were compared and successfully applied to analyze the monosaccharide 
      contents of a purified acidic heteropolysaccharide previously isolated from 
      Ephedra sinica. The established UHPLC-ESI-MS and QAMS approach possesses high 
      precision, stability and repeatability, and can be widely used for 
      high-throughput analysis of the monosaccharide compositions of plant 
      polysaccharides.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Li, Ye
AU  - Li Y
AD  - Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese 
      Medicine), Ministry of Education, 24 Heping Road, Harbin, 150040, PR China.
FAU - Liang, Jun
AU  - Liang J
AD  - Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese 
      Medicine), Ministry of Education, 24 Heping Road, Harbin, 150040, PR China.
FAU - Shen, Yu
AU  - Shen Y
AD  - Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese 
      Medicine), Ministry of Education, 24 Heping Road, Harbin, 150040, PR China.
FAU - Kuang, Hai-Xue
AU  - Kuang HX
AD  - Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese 
      Medicine), Ministry of Education, 24 Heping Road, Harbin, 150040, PR China. 
      Electronic address: hxkuang56@163.com.
FAU - Xia, Yong-Gang
AU  - Xia YG
AD  - Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese 
      Medicine), Ministry of Education, 24 Heping Road, Harbin, 150040, PR China. 
      Electronic address: yonggangxia@163.com.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20200528
PL  - England
TA  - Carbohydr Polym
JT  - Carbohydrate polymers
JID - 8307156
RN  - 0 (Monosaccharides)
RN  - 0 (Plant Extracts)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Acetylation
MH  - Chromatography, High Pressure Liquid/methods
MH  - Ephedra sinica/*chemistry
MH  - Hydrogen-Ion Concentration
MH  - Monosaccharides/*analysis
MH  - Plant Extracts/*analysis
MH  - Polysaccharides/*analysis
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
OTO - NOTNLM
OT  - Polysaccharide
OT  - QAMS
OT  - UHPLC-ESI-MS
OT  - monosaccharide compositions
COIS- Declaration of Competing Interest No conflict of interest exists in the 
      submission of this manuscript.
EDAT- 2020/07/29 06:00
MHDA- 2021/04/15 06:00
CRDT- 2020/07/29 06:00
PHST- 2020/04/07 00:00 [received]
PHST- 2020/05/03 00:00 [revised]
PHST- 2020/05/08 00:00 [accepted]
PHST- 2020/07/29 06:00 [entrez]
PHST- 2020/07/29 06:00 [pubmed]
PHST- 2021/04/15 06:00 [medline]
AID - S0144-8617(20)30613-5 [pii]
AID - 10.1016/j.carbpol.2020.116439 [doi]
PST - ppublish
SO  - Carbohydr Polym. 2020 Oct 1;245:116439. doi: 10.1016/j.carbpol.2020.116439. Epub 
      2020 May 28.