PMID- 32724297
OWN - NLM
STAT- MEDLINE
DCOM- 20210719
LR  - 20210719
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2020
DP  - 2020
TI  - Intraoperative Peritoneal Interleukin-6 Concentration Changes in Relation to the 
      High-Mobility Group Protein B1 and Heat Shock Protein 70 Levels in Children 
      Undergoing Cholecystectomy.
PG  - 9613105
LID - 10.1155/2020/9613105 [doi]
LID - 9613105
AB  - The aim was the evaluation of IL-6 concentration in peritoneal lavage fluid of 
      children which underwent cholecystectomy to ascertain if there is a difference in 
      early inflammatory response depending on the type of surgical approach (open vs. 
      laparoscopy). The analysis of high-mobility group protein B1 (HMGB1) and heat 
      shock protein 70 (HSP70) was performed to find out if the source of IL-6 was 
      related to tissue damage. IL-6 concentration in peritoneal lavage fluid samples, 
      obtained at the beginning and at the end of the laparoscopic (N = 23) and open 
      cholecystectomy (N = 14), was tested with a routinely used 
      electrochemiluminescence assay. The concentrations of HMGB1 and HSP70 were 
      analyzed with the use of an ELISA method. Statistical analysis was performed 
      using the STATISTICA PL release 12.5 Program. The differences were assessed using 
      the Mann-Whitney U test and Wilcoxon matched pairs test. Correlations were 
      studied by using the Spearman correlation test. Our results demonstrated 
      significant peritoneal lavage fluid IL-6 concentration growth measured at the end 
      of the cholecystectomy as compared to the beginning, regardless of the type of 
      the procedure. IL-6 growth during open cholecystectomy was greater compared to 
      laparoscopic cholecystectomy (62.51-fold vs. 3.19-fold). IL-6 concentration did 
      not correlate with HMGB1 and HSP70, which indicate that the significant growth of 
      this cytokine was not related to mechanical tissue damage due to surgical 
      procedure. A clinical significance of the study could be related to the fact that 
      the evaluation of IL-6 concentration in peritoneal lavage fluid may be useful to 
      assess an early local inflammatory response.
CI  - Copyright (c) 2020 Marzena Tylicka et al.
FAU - Tylicka, Marzena
AU  - Tylicka M
AUID- ORCID: 0000-0002-9594-8173
AD  - Department of Biophysics, Medical University of Bialystok, Mickiewicza 2a, 15-089 
      Bialystok, Poland.
FAU - Matuszczak, Ewa
AU  - Matuszczak E
AUID- ORCID: 0000-0003-2425-9589
AD  - Department of Pediatric Surgery and Urology, Medical University of Bialystok, 
      Waszyngtona 17, 15-274 Bialystok, Poland.
FAU - Kaminska, Joanna
AU  - Kaminska J
AUID- ORCID: 0000-0002-2986-4852
AD  - Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, 
      Waszyngtona 15A, 15-269 Bialystok, Poland.
FAU - Debek, Wojciech
AU  - Debek W
AD  - Department of Pediatric Surgery and Urology, Medical University of Bialystok, 
      Waszyngtona 17, 15-274 Bialystok, Poland.
FAU - Modzelewska, Beata
AU  - Modzelewska B
AUID- ORCID: 0000-0002-1051-7906
AD  - Department of Biophysics, Medical University of Bialystok, Mickiewicza 2a, 15-089 
      Bialystok, Poland.
FAU - Kleszczewski, Tomasz
AU  - Kleszczewski T
AD  - Department of Biophysics, Medical University of Bialystok, Mickiewicza 2a, 15-089 
      Bialystok, Poland.
FAU - Dymicka-Piekarska, Violetta
AU  - Dymicka-Piekarska V
AD  - Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, 
      Waszyngtona 15A, 15-269 Bialystok, Poland.
FAU - Matowicka-Karna, Joanna
AU  - Matowicka-Karna J
AUID- ORCID: 0000-0003-4438-9110
AD  - Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, 
      Waszyngtona 15A, 15-269 Bialystok, Poland.
FAU - Karpinska, Maria
AU  - Karpinska M
AD  - Department of Biophysics, Medical University of Bialystok, Mickiewicza 2a, 15-089 
      Bialystok, Poland.
FAU - Koper-Lenkiewicz, Olga M
AU  - Koper-Lenkiewicz OM
AUID- ORCID: 0000-0001-5199-2773
AD  - Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, 
      Waszyngtona 15A, 15-269 Bialystok, Poland.
LA  - eng
PT  - Journal Article
DEP - 20200708
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (HMGB1 Protein)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Child
MH  - *Cholecystectomy
MH  - Cholecystectomy, Laparoscopic
MH  - Enzyme-Linked Immunosorbent Assay
MH  - HMGB1 Protein/*metabolism
MH  - HSP70 Heat-Shock Proteins/*metabolism
MH  - Humans
MH  - Interleukin-6/*metabolism
MH  - Peritoneum/*metabolism
PMC - PMC7366196
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2020/07/30 06:00
MHDA- 2021/07/20 06:00
PMCR- 2020/07/08
CRDT- 2020/07/30 06:00
PHST- 2020/04/15 00:00 [received]
PHST- 2020/06/04 00:00 [revised]
PHST- 2020/06/19 00:00 [accepted]
PHST- 2020/07/30 06:00 [entrez]
PHST- 2020/07/30 06:00 [pubmed]
PHST- 2021/07/20 06:00 [medline]
PHST- 2020/07/08 00:00 [pmc-release]
AID - 10.1155/2020/9613105 [doi]
PST - epublish
SO  - Mediators Inflamm. 2020 Jul 8;2020:9613105. doi: 10.1155/2020/9613105. 
      eCollection 2020.