PMID- 32724429
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 20
IP  - 2
DP  - 2020 Aug
TI  - Association between TP53 gene deletion and protein expression in esophageal 
      squamous cell carcinoma and its prognostic significance.
PG  - 1855-1865
LID - 10.3892/ol.2020.11709 [doi]
AB  - The aim of the present study was to investigate the association between tumor 
      protein 53 (TP53) gene deletion and protein expression and clinical features in 
      esophageal squamous cell carcinoma (ESCC), and to evaluate the predictive value 
      of these two characteristics in the prognosis of ESCC. Immunohistochemistry (IHC) 
      and fluorescence in situ hybridization (FISH) were performed to detect the 
      expression of p53 protein and gene deletion in ESCC tissue samples from different 
      ethnic groups in Xinjiang, in order to analyze their association with 
      clinicopathological characteristics and patient prognosis, as well as the 
      sensitivity and specificity of the two methods. In addition, the results were 
      further validated by tissue microarray from a different region. The positive rate 
      of p53 protein expression was 54.5% (201/369) in the multi-ethnic group, and was 
      significantly different between sex (P=0.026) and between tumor differentiation 
      groups (P=0.032). FISH demonstrated that the TP53 gene deletion rate was 31.8% 
      (68/214), which was significantly different between different tumor 
      differentiation (P=0.002), lymph node metastasis (P=0.005) and vascular invasion 
      (P<0.001) groups. The survival rate of patients with TP53 gene deletion was 
      significantly lower than those without TP53 gene deletion (P<0.05). The positive 
      rate of p53 protein expression in the tissue microarray was 58.1% (68/117), which 
      was significantly different between the depth of invasion groups (P=0.011). The 
      TP53 gene deletion rate was 47.9% (56/117), which significantly differed 
      according to lymph node metastasis (P=0.003) and TNM stage (P=0.01). In addition, 
      the total concordance rates of the two methods were 60.3 and 64.1%, respectively. 
      There were also significant differences in the positive rate of TP53 gene 
      deletion and protein expression in different stages of ESCC (P<0.05), which 
      increased gradually with the progression of ESCC. The deletion of the TP53 gene 
      in esophageal cancer was associated with poor prognosis and may be an important 
      biomarker for evaluating the prognosis of patients with ESCC. The combination of 
      FISH and IHC methods could significantly improve the detection rate of TP53 gene 
      abnormalities and the accuracy of prognostic assessment of ESCC.
CI  - Copyright: (c) Niyaz et al.
FAU - Niyaz, Madiniyet
AU  - Niyaz M
AD  - Clinical Medicine Research Institute, The First Affiliated Hospital of Xinjiang 
      Medical University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Ainiwaer, Julaiti
AU  - Ainiwaer J
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Abudureheman, Abulajiang
AU  - Abudureheman A
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Zhang, Liwei
AU  - Zhang L
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Sheyhidin, Ilyar
AU  - Sheyhidin I
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Turhong, Abduheny
AU  - Turhong A
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Cai, Ren
AU  - Cai R
AD  - Clinical Medicine Research Institute, The First Affiliated Hospital of Xinjiang 
      Medical University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Hou, Zhichao
AU  - Hou Z
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Awut, Edris
AU  - Awut E
AD  - Department of Thoracic Surgery, The First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200609
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC7377104
OTO - NOTNLM
OT  - deletion
OT  - esophageal squamous cell carcinoma
OT  - fluorescence in situ hybridization
OT  - immunohistochemistry
OT  - prognosis
OT  - tumor protein 53
EDAT- 2020/07/30 06:00
MHDA- 2020/07/30 06:01
PMCR- 2020/06/09
CRDT- 2020/07/30 06:00
PHST- 2019/06/24 00:00 [received]
PHST- 2020/04/20 00:00 [accepted]
PHST- 2020/07/30 06:00 [entrez]
PHST- 2020/07/30 06:00 [pubmed]
PHST- 2020/07/30 06:01 [medline]
PHST- 2020/06/09 00:00 [pmc-release]
AID - OL-0-0-11709 [pii]
AID - 10.3892/ol.2020.11709 [doi]
PST - ppublish
SO  - Oncol Lett. 2020 Aug;20(2):1855-1865. doi: 10.3892/ol.2020.11709. Epub 2020 Jun 
      9.