PMID- 32725513 OWN - NLM STAT- MEDLINE DCOM- 20210921 LR - 20220210 IS - 1573-2576 (Electronic) IS - 0360-3997 (Linking) VI - 43 IP - 6 DP - 2020 Dec TI - Panaxatriol Saponins Promote M2 Polarization of BV2 Cells to Reduce Inflammation and Apoptosis after Glucose/Oxygen Deprivation by Activating STAT3. PG - 2109-2118 LID - 10.1007/s10753-020-01278-x [doi] AB - Panaxatriol saponins (PTS) have a long history in the treatment of stroke. In our previous experiments, PTS has been found to alleviate ischemic stroke and play a role through regulating the inflammatory response, but the specific mechanism of its regulation is still unclear. Cell viability was determined by MTT assay. Expressions of polarization-related proteins CD16, CD68, ARG1 and CD206; inflammatory factors interleukin-1beta (IL-1beta); inducible nitric oxide synthase (iNOS); monocyte chemotactic protein 1(MCP-1) and cyclooxygenase-2 (COX-2); apoptosis-related proteins pro-caspase3; bax; caspase3 and bcl-2; and STAT3 and p-STAT3 were detected by western blot. ELISA was used to detect the expression of inflammatory-related factors in cells. The apoptosis rate was detected by flow cytometry. We found that the survival rate of oxygen sugar deprivation/reoxygenation (OGD/R) cells increased obviously after PTS treatment in a dose-dependent manner. PTS can promote M2 polarization of microglial cells (BV2) and inhibit inflammatory response of OGD/R cells, accompanied by decreased expression of inflammatory factors IL-1beta, iNOS, MCP-1, and COX-2. PTS inhibited apoptosis of OGD/R cells and was accompanied by decreased expression of apoptotic proteins Bax and caspase3 and increased expression of Bcl-2. We also found that PTS activated STAT3 levels in BV2 cells. After the addition of STAT3 inhibitor Stattic, it was found that PTS could promote M2 polarization of BV2 cells by activating the STAT3 pathway, thus inhibiting cell inflammation and apoptosis. PTS promoted M2 polarization in microglia cells by activating the STAT3 pathway, thereby reducing cell inflammation and apoptosis after glucose/oxygen deprivation. FAU - Li, Chaosheng AU - Li C AD - Department of Neurology, Affiliated Hospital of Jiangnan University, Wuxi, 214000, Jiangsu, China. AD - Wuxi Clinical Medicine School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Wuxi, 214000, Jiangsu, China. AD - The Third Hospital Affiliated to Nantong University, Wuxi, 214000, Jiangsu, China. FAU - Fan, Changyan AU - Fan C AD - Department of Neurology, Affiliated Hospital of Jiangnan University, Wuxi, 214000, Jiangsu, China. AD - Wuxi Clinical Medicine School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Wuxi, 214000, Jiangsu, China. AD - The Third Hospital Affiliated to Nantong University, Wuxi, 214000, Jiangsu, China. FAU - Zhao, Jilai AU - Zhao J AD - Department of Neurology, Affiliated Hospital of Jiangnan University, Wuxi, 214000, Jiangsu, China. AD - Wuxi Clinical Medicine School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Wuxi, 214000, Jiangsu, China. AD - The Third Hospital Affiliated to Nantong University, Wuxi, 214000, Jiangsu, China. FAU - Di, Meiqi AU - Di M AD - Department of Neurology, Affiliated Hospital of Jiangnan University, Wuxi, 214000, Jiangsu, China. AD - Wuxi Clinical Medicine School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Wuxi, 214000, Jiangsu, China. AD - The Third Hospital Affiliated to Nantong University, Wuxi, 214000, Jiangsu, China. FAU - Sui, Chenyan AU - Sui C AD - Department of Neurology, Affiliated Hospital of Jiangnan University, Wuxi, 214000, Jiangsu, China. AD - Wuxi Clinical Medicine School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Wuxi, 214000, Jiangsu, China. AD - The Third Hospital Affiliated to Nantong University, Wuxi, 214000, Jiangsu, China. FAU - Han, Likun AU - Han L AD - Department of Neurology, Affiliated Hospital of Jiangnan University, Wuxi, 214000, Jiangsu, China. AD - Wuxi Clinical Medicine School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Wuxi, 214000, Jiangsu, China. AD - The Third Hospital Affiliated to Nantong University, Wuxi, 214000, Jiangsu, China. FAU - Hu, Lingling AU - Hu L AUID- ORCID: 0000-0001-5224-8305 AD - Department of Neurology, Affiliated Hospital of Jiangnan University, Wuxi, 214000, Jiangsu, China. hulingling209@163.com. AD - Wuxi Clinical Medicine School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Wuxi, 214000, Jiangsu, China. hulingling209@163.com. AD - The Third Hospital Affiliated to Nantong University, Wuxi, 214000, Jiangsu, China. hulingling209@163.com. LA - eng GR - QNRC084/Scientific research project of Wuxi Board of Health/ GR - MS201955/Scientific research project of Wuxi Board of Health/ PT - Journal Article PL - United States TA - Inflammation JT - Inflammation JID - 7600105 RN - 0 (Cytokines) RN - 0 (Ginsenosides) RN - 0 (Neuroprotective Agents) RN - 0 (STAT3 Transcription Factor) RN - 0 (Saponins) RN - 0 (Stat3 protein, mouse) RN - 9F5B25FDDG (panaxatriol) RN - IY9XDZ35W2 (Glucose) RN - S88TT14065 (Oxygen) SB - IM MH - Animals MH - Apoptosis/*drug effects MH - Cell Differentiation MH - Cell Line MH - Cell Survival/drug effects MH - Cytokines/biosynthesis/metabolism MH - Ginsenosides/*pharmacology MH - Glucose/*metabolism MH - Inflammation/drug therapy MH - Macrophages/*drug effects MH - Mice MH - Microglia/drug effects MH - Neuroprotective Agents/pharmacology MH - Oxygen/metabolism MH - STAT3 Transcription Factor/biosynthesis/*metabolism MH - Saponins/*pharmacology MH - Signal Transduction/drug effects OTO - NOTNLM OT - BV2 cells OT - M2 polarization OT - STAT3 OT - apoptosis OT - glucose/oxygen deprivation OT - inflammation OT - panaxatriol saponins EDAT- 2020/07/30 06:00 MHDA- 2021/09/22 06:00 CRDT- 2020/07/30 06:00 PHST- 2020/07/30 06:00 [pubmed] PHST- 2021/09/22 06:00 [medline] PHST- 2020/07/30 06:00 [entrez] AID - 10.1007/s10753-020-01278-x [pii] AID - 10.1007/s10753-020-01278-x [doi] PST - ppublish SO - Inflammation. 2020 Dec;43(6):2109-2118. doi: 10.1007/s10753-020-01278-x.