PMID- 32726143
OWN - NLM
STAT- MEDLINE
DCOM- 20201130
LR  - 20201130
IS  - 1522-1504 (Electronic)
IS  - 1040-0605 (Linking)
VI  - 319
IP  - 4
DP  - 2020 Oct 1
TI  - Cobalt exposure via skin alters lung immune cells and enhances pulmonary 
      responses to cobalt in mice.
PG  - L641-L651
LID - 10.1152/ajplung.00265.2020 [doi]
AB  - Cobalt has been associated with allergic contact dermatitis and occupational 
      asthma. However, the link between skin exposure and lung responses to cobalt is 
      currently unknown. We investigated the effect of prior dermal sensitization to 
      cobalt on pulmonary physiological and immunological responses after subsequent 
      challenge with cobalt via the airways. BALB/c mice received epicutaneous 
      applications (25 muL/ear) with 5% CoCl(2*)6H(2)O (Co) or the vehicle (Veh) 
      dimethyl sulfoxide (DMSO) twice; they then received oropharyngeal challenges with 
      0.05% CoCl(2*)6H(2)O or saline five times, thereby obtaining four groups: 
      Veh/Veh, Co/Veh, Veh/Co, and Co/Co. To detect early respiratory responses 
      noninvasively, we performed sequential in vivo microcomputed tomography (microCT). 
      One day after the last challenge, we assessed airway hyperreactivity (AHR) to 
      methacholine, inflammation in bronchoalveolar lavage (BAL), innate lymphoid cells 
      (ILCs) and dendritic cells (DCs) in the lungs, and serum IgE. Compared with the 
      Veh/Veh group, the Co/Co group showed increased microCT-derived lung response, 
      increased AHR to methacholine, mixed neutrophilic and eosinophilic inflammation, 
      elevated monocyte chemoattractant protein-1 (MCP-1), and elevated keratinocyte 
      chemoattractant (KC) in BAL. Flow cytometry in the Co/Co group demonstrated 
      increased DC, type 1 and type 2 conventional DC (cDC1/cDC2), monocyte-derived DC, 
      increased ILC group 2, and natural cytotoxicity receptor(-)ILC group 3. The 
      Veh/Co group showed only increased AHR to methacholine and elevated MCP-1 in BAL, 
      whereas the Co/Veh group showed increased cDC1 and ILC2 in lung. We conclude that 
      dermal sensitization to cobalt may increase the susceptibility of the lungs to 
      inhaling cobalt. Mechanistically, this enhanced susceptibility involves changes 
      in pulmonary DCs and ILCs.
FAU - Tsui, Hung-Chang
AU  - Tsui HC
AUID- ORCID: 0000-0002-0105-5455
AD  - Centre for Environment and Health, Department of Public Health and Primary Care, 
      KU Leuven, Leuven, Belgium.
FAU - Decaesteker, Tatjana
AU  - Decaesteker T
AD  - Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), Department of 
      Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium.
FAU - Jonckheere, Anne-Charlotte
AU  - Jonckheere AC
AD  - Allergy and Clinical Immunology Research Group, Department of Microbiology and 
      Immunology, KU Leuven, Leuven, Belgium.
FAU - Vande Velde, Greetje
AU  - Vande Velde G
AD  - Biomedical MRI, Department of Imaging and Pathology, KU Leuven, Leuven, Belgium.
FAU - Cremer, Jonathan
AU  - Cremer J
AD  - Translational Research in Gastrointestinal Disorders (TARGID), Department of 
      Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium.
FAU - Verbeken, Erik
AU  - Verbeken E
AD  - Translational Cell and Tissue Research, Department of Imaging and Pathology, KU 
      Leuven, Leuven, Belgium.
FAU - Hoet, Peter H M
AU  - Hoet PHM
AD  - Centre for Environment and Health, Department of Public Health and Primary Care, 
      KU Leuven, Leuven, Belgium.
FAU - Nemery, Benoit
AU  - Nemery B
AD  - Centre for Environment and Health, Department of Public Health and Primary Care, 
      KU Leuven, Leuven, Belgium.
FAU - Vanoirbeek, Jeroen A J
AU  - Vanoirbeek JAJ
AD  - Centre for Environment and Health, Department of Public Health and Primary Care, 
      KU Leuven, Leuven, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200729
PL  - United States
TA  - Am J Physiol Lung Cell Mol Physiol
JT  - American journal of physiology. Lung cellular and molecular physiology
JID - 100901229
RN  - 0W5ETF9M2K (Methacholine Chloride)
RN  - 3G0H8C9362 (Cobalt)
SB  - IM
MH  - Animals
MH  - Bronchial Hyperreactivity/*drug therapy/immunology
MH  - Bronchoalveolar Lavage/methods
MH  - Bronchoalveolar Lavage Fluid/immunology
MH  - Cobalt/*pharmacology
MH  - Disease Models, Animal
MH  - Inflammation/chemically induced/*drug therapy
MH  - Lung/drug effects/immunology
MH  - Lymphocytes/*drug effects/immunology
MH  - Methacholine Chloride/metabolism
MH  - Mice, Inbred BALB C
OTO - NOTNLM
OT  - cobalt
OT  - dendritic cell
OT  - innate lymphoid cell
OT  - occupational asthma
OT  - skin exposure
EDAT- 2020/07/30 06:00
MHDA- 2020/12/01 06:00
CRDT- 2020/07/30 06:00
PHST- 2020/07/30 06:00 [pubmed]
PHST- 2020/12/01 06:00 [medline]
PHST- 2020/07/30 06:00 [entrez]
AID - 10.1152/ajplung.00265.2020 [doi]
PST - ppublish
SO  - Am J Physiol Lung Cell Mol Physiol. 2020 Oct 1;319(4):L641-L651. doi: 
      10.1152/ajplung.00265.2020. Epub 2020 Jul 29.