PMID- 32729420
OWN - NLM
STAT- MEDLINE
DCOM- 20211124
LR  - 20211124
IS  - 1875-6360 (Electronic)
IS  - 1573-3971 (Linking)
VI  - 17
IP  - 1
DP  - 2021
TI  - Dietary Antioxidants Significantly Reduced Phorbol Myristate Acetate Induced 
      Oxidative Stress of Peripheral Blood Mononuclear Cells of Patients with 
      Rheumatoid Arthritis.
PG  - 81-87
LID - 10.2174/1573397116999200729154954 [doi]
AB  - BACKGROUND: Rheumatoid arthritis (RA) is an autoimmune disease responsible for 
      maximum human morbidity in modern life, whereas oxidative stress is the ultimate 
      potential biomarker for determining disease activity in patients with RA. 
      OBJECTIVE: The present study scientifically validated the effectiveness of 
      antioxidants commonly present in different food supplements to neutralize the 
      free radicals mediated oxidative stress in isolated peripheral blood mononuclear 
      lymphocytes (PBML) of patients with RA. METHODS: The study population included 
      patients with Rheumatoid arthritis, RA (n =15), who fulfilled the American 
      College of Rheumatology criteria for RA. Peripheral blood was collected, and 
      isolated mononuclear lymphocyte cells (PBML) were pretreated with phorbol 
      myristate acetate (PMS) and furthermore, incubated with different concentrations 
      of Naringenin, beta carotene and Nacetyl cysteine (NAC) in an ex vivo condition. The 
      resultant cell lysate was used for further studies for the determination of other 
      oxidative biomarkers. The increase of superoxide and nitric oxide production was 
      observed when PBML was treated PMS. RESULTS: Importantly, the increased oxidative 
      stress was effectively decreased by the selected plantderived compounds 
      beta-carotene and naringenin. CONCLUSION: The study scientifically evaluated the 
      efficacy of the molecules validated by one-way ANOVA, followed by Dunnett's post 
      hoc test of significance. Collectively, our results indicate that both beta carotene 
      and naringenin may be a promising non-toxic food supplement in attenuating the 
      oxidative stress associated pathology in RA, meriting further pharmacological 
      studies on other inflammatory cells like neutrophils.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Chetia, Purbajit
AU  - Chetia P
AD  - Department of Pharmacology, Himalayan Pharmacy Institute, Majhitar, Rangpo, East 
      Sikkim 737136, Sikkim, India.
FAU - Khandelwal, Bidita
AU  - Khandelwal B
AD  - Department of Medicine, Sikkim Manipal Institute of Medical Science, 5th Mile 
      Tadong, Gangtok 737102, Sikkim, India.
FAU - Haldar, Pallab Kanti
AU  - Haldar PK
AD  - Department of Pharmaceutical Technology, Jadavpur University, 188B Raja SC 
      Mullick Road, Kolkata 700032, West Bengal, India.
FAU - Bala, Asis
AU  - Bala A
AD  - Division of Pharmacology, Guru Nanak Institute of Pharmaceutical Science and 
      Technology, Sodepur, Pin- 700114, West Bengal, India.
LA  - eng
PT  - Journal Article
PL  - United Arab Emirates
TA  - Curr Rheumatol Rev
JT  - Current rheumatology reviews
JID - 101261938
RN  - 0 (Antioxidants)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adult
MH  - Antioxidants/*pharmacology
MH  - Arthritis, Rheumatoid/blood/*metabolism
MH  - Cells, Cultured
MH  - Dietary Supplements
MH  - Female
MH  - Humans
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Oxidative Stress/*drug effects
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Young Adult
OTO - NOTNLM
OT  - Rheumatoid arthritis
OT  - antioxidants
OT  - mononuclear lymphocytes
OT  - naringenin
OT  - oxidative stress
OT  - beta carotene and Nacetyl cysteine (NAC)
EDAT- 2020/07/31 06:00
MHDA- 2021/11/25 06:00
CRDT- 2020/07/31 06:00
PHST- 2020/04/01 00:00 [received]
PHST- 2020/06/24 00:00 [revised]
PHST- 2020/06/29 00:00 [accepted]
PHST- 2020/07/31 06:00 [pubmed]
PHST- 2021/11/25 06:00 [medline]
PHST- 2020/07/31 06:00 [entrez]
AID - CRR-EPUB-108662 [pii]
AID - 10.2174/1573397116999200729154954 [doi]
PST - ppublish
SO  - Curr Rheumatol Rev. 2021;17(1):81-87. doi: 10.2174/1573397116999200729154954.