PMID- 32729803
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
DP  - 2020 Jul 30
TI  - Lilac Tasselflower (Emilia sonchifolia) is a New Host for Peanut Witches' Broom 
      Phytoplasma, a 16SrII-V Subgroup Strain in Taiwan.
LID - 10.1094/PDIS-06-20-1304-PDN [doi]
AB  - Lilac tasselflower (Emilia sonchifolia) is an annual herbaceous plant that 
      belongs to the family of Asteraceae. Lilac tasselflower is widely distributed at 
      mid-low altitude regions in Taiwan, and is commonly used as traditional herbal 
      medicine for the treatment of inflammation, rheumatism, dysentery, and analgesic. 
      In March 2020, disease symptoms such as shoot proliferation, phyllody, and 
      witches' broom were observed on lilac tasselflower at the sansheng community park 
      in Mailiao, Yunlin County, Taiwan. Totally, four lilac tasselflower plants were 
      checked and half of them were symptomatic. At the same area, similar symptoms 
      associated with peanut witches' broom (PnWB) disease were observed (Liu et al. 
      2015). Samples including one healthy and two symptomatic lilac tasselflower were 
      collected for total DNA and protein extraction used for PCR and western blotting 
      assays, respectively. First, two sets of phytoplasma universal primer pairs P1/P7 
      and R16F2n/R16R2 were used to perform nested PCR for detection of 16S ribosomal 
      RNA (rRNA) gene (Lee et al. 1993). A specific signal of expected size (1.2 kb) 
      for 16S rRNA was only detected in samples of lilac tasselflower exhibiting 
      disease symptoms. The amplified DNA fragment using primer pairs P1/P7 was 
      partially sequenced (accession no. MT420682) with P1 and a nested primer 
      (5'-GGGTCTTTACTGACGCTGAGG-3'). The 1.4 kb nucleotide sequence shares 100% 
      identity with that of GenBank accession NZ_AMWZ01000008 (complement [31109 to 
      32640]) of phytoplasma associated with PnWB disease (Chung et al. 2013). Further 
      analysis by iPhyClassifier, the virtual RFLP pattern of MT420682 confirmed that 
      the phytoplasma detected in symptomatic lilac tasselflower could be classified 
      into the 16SrII-V subgroup. For western blotting, total protein of each sample 
      was examined using the polyclonal antibody raised against Imp protein of purple 
      coneflower witches' broom phytoplasma (Chien et al. 2020), which shares 100% 
      identity with that (accession no. ADD59806) of PnWB phytoplasma. A specific 
      signal of expected size (19 kDa) for Imp was detected in symptomatic lilac 
      tasselflower, but not in healthy lilac tasselflower. Subsequent PCR, DNA 
      sequencing and western blotting assays further confirmed that the gene encoding a 
      SAP11-like protein detected in samples of lilac tasselflower exhibiting disease 
      symptoms is identical to that (accession no. EMR14684) of PnWB phytoplasma. Our 
      results indicated that lilac tasselflower, which is recognized as a common weed 
      in Taiwan, may facilitate the spreading of phytoplasma disease by acting as an 
      alternative natural host for PnWB phytoplasma.
FAU - Chien, Yuanyu
AU  - Chien Y
AD  - Graduate Institute of Biochemistry, National Chung Hsing University, Taichung 
      City, Taiwan; papaya1032571@gmail.com.
FAU - Tan, Choon-Meng
AU  - Tan CM
AD  - Graduate Institute of Biochemistry, National Chung Hsing University, Taichung, 
      Taiwan; choonmeng617@hotmail.com.
FAU - Kung, Yueh-Chen
AU  - Kung YC
AD  - Graduate Institute of Biochemistry, National Chung Hsing University, Taichung, 
      Taiwan; yckung0103@gmail.com.
FAU - Lee, Ya-Chien
AU  - Lee YC
AD  - Graduate Institute of Biochemistry, National Chung Hsing University, Taichung, 
      Taiwan; edithlee075@gmail.com.
FAU - Chiu, Yi-Ching
AU  - Chiu YC
AD  - Graduate Institute of Biochemistry, National Chung Hsing University, Taichung, 
      Taiwan; jean31019@gmail.com.
FAU - Yang, Jun-Yi
AU  - Yang JY
AD  - Institute of Biochemistry, National Chung Hsing University, Taichung, Taiwan.
AD  - Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung, 
      Taiwan; jyang@nchu.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20200730
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
SB  - IM
OTO - NOTNLM
OT  - Lilac tasselflower
OT  - Peanut witches' broom disease
OT  - Phytoplasma
OT  - SAP11
EDAT- 2020/07/31 06:00
MHDA- 2020/07/31 06:00
CRDT- 2020/07/31 06:00
PHST- 2020/07/31 06:00 [entrez]
PHST- 2020/07/31 06:00 [pubmed]
PHST- 2020/07/31 06:00 [medline]
AID - 10.1094/PDIS-06-20-1304-PDN [doi]
PST - aheadofprint
SO  - Plant Dis. 2020 Jul 30. doi: 10.1094/PDIS-06-20-1304-PDN.