PMID- 32731411
OWN - NLM
STAT- MEDLINE
DCOM- 20210310
LR  - 20210310
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 12
IP  - 8
DP  - 2020 Jul 28
TI  - Barrier Protection and Recovery Effects of Gut Commensal Bacteria on 
      Differentiated Intestinal Epithelial Cells In Vitro.
LID - 10.3390/nu12082251 [doi]
LID - 2251
AB  - Alterations in the gut microbiota composition play a crucial role in the 
      pathogenesis of inflammatory bowel disease (IBD) as specific commensal bacterial 
      species are underrepresented in the microbiota of IBD patients. In this study, we 
      examined the therapeutic potential of three commensal bacterial species, 
      Faecalibacterium prausnitzii (F. prausnitzii), Roseburia intestinalis (R. 
      intestinalis) and Bacteroides faecis (B. faecis) in an in vitro model of 
      intestinal inflammation, by using differentiated Caco-2 and HT29-MTX cells, 
      stimulated with a pro-inflammatory cocktail consisting of interleukin-1beta (IL-1beta), 
      tumor necrosis factor-alpha (TNFalpha), interferon-gamma (IFNgamma), and lipopolysaccharide 
      (LPS). Results obtained in this work demonstrated that all three bacterial 
      species are able to recover the impairment of the epithelial barrier function 
      induced by the inflammatory stimulus, as determined by an amelioration of the 
      transepithelial electrical resistance (TEER) and the paracellular permeability of 
      the cell monolayer. Moreover, inflammatory stimulus increased claudin-2 
      expression and decreased occludin expression were improved in the cells treated 
      with commensal bacteria. Furthermore, the commensals were able to counteract the 
      increased release of interleukin-8 (IL-8) and monocyte chemoattractant protein-1 
      (MCP-1) induced by the inflammatory stimulus. These findings indicated that F. 
      prausnitzii, R. intestinalis and B. faecis improve the epithelial barrier 
      integrity and limit inflammatory responses.
FAU - Mohebali, Nooshin
AU  - Mohebali N
AD  - Molecular Bacteriology, Institute of Medical Microbiology, Virology and Hygiene, 
      University Medicine Rostock, 18057 Rostock, Germany.
FAU - Ekat, Katharina
AU  - Ekat K
AD  - Molecular Bacteriology, Institute of Medical Microbiology, Virology and Hygiene, 
      University Medicine Rostock, 18057 Rostock, Germany.
FAU - Kreikemeyer, Bernd
AU  - Kreikemeyer B
AUID- ORCID: 0000-0001-9527-5098
AD  - Molecular Bacteriology, Institute of Medical Microbiology, Virology and Hygiene, 
      University Medicine Rostock, 18057 Rostock, Germany.
FAU - Breitruck, Anne
AU  - Breitruck A
AD  - Molecular Bacteriology, Institute of Medical Microbiology, Virology and Hygiene, 
      University Medicine Rostock, 18057 Rostock, Germany.
LA  - eng
GR  - 2017-01/Damp Stiftung/
PT  - Journal Article
DEP - 20200728
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Claudin-2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Occludin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
RN  - Bacteroides faecis
RN  - Roseburia intestinalis
SB  - IM
MH  - *Bacteroides
MH  - Caco-2 Cells
MH  - Chemokine CCL2/metabolism
MH  - Claudin-2
MH  - *Clostridiales
MH  - Electric Impedance
MH  - Epithelial Cells/*microbiology
MH  - *Faecalibacterium prausnitzii
MH  - Gastrointestinal Microbiome/*physiology
MH  - HT29 Cells
MH  - Humans
MH  - Inflammatory Bowel Diseases/*microbiology
MH  - Interferon-gamma/administration & dosage
MH  - Interleukin-1beta/administration & dosage
MH  - Interleukin-8/metabolism
MH  - Intestinal Mucosa/microbiology
MH  - Lipopolysaccharides/administration & dosage
MH  - Occludin/metabolism
MH  - Permeability
MH  - Tumor Necrosis Factor-alpha/administration & dosage
PMC - PMC7468801
OTO - NOTNLM
OT  - Bacteroides faecis
OT  - Caco-2 cells
OT  - Faecalibacterium prausnitzii
OT  - HT29-MTX cells
OT  - IBD
OT  - Roseburia intestinalis
OT  - commensal bacteria
OT  - tight junction proteins
OT  - transepithelial electrical resistance (TEER)
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/08/01 06:00
MHDA- 2021/03/11 06:00
PMCR- 2020/08/01
CRDT- 2020/08/01 06:00
PHST- 2020/06/26 00:00 [received]
PHST- 2020/07/21 00:00 [revised]
PHST- 2020/07/22 00:00 [accepted]
PHST- 2020/08/01 06:00 [entrez]
PHST- 2020/08/01 06:00 [pubmed]
PHST- 2021/03/11 06:00 [medline]
PHST- 2020/08/01 00:00 [pmc-release]
AID - nu12082251 [pii]
AID - nutrients-12-02251 [pii]
AID - 10.3390/nu12082251 [doi]
PST - epublish
SO  - Nutrients. 2020 Jul 28;12(8):2251. doi: 10.3390/nu12082251.