PMID- 32731586
OWN - NLM
STAT- MEDLINE
DCOM- 20210224
LR  - 20210224
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 12
IP  - 8
DP  - 2020 Jul 28
TI  - Host Transcriptional Response to Persistent Infection with a Live-Attenuated 
      Porcine Reproductive and Respiratory Syndrome Virus Strain.
LID - 10.3390/v12080817 [doi]
LID - 817
AB  - Both virulent and live-attenuated porcine reproductive and respiratory syndrome 
      virus (PRRSV) strains can establish persistent infection in lymphoid tissues of 
      pigs. To investigate the mechanisms of PRRSV persistence, we performed a 
      transcriptional analysis of inguinal lymphoid tissue collected from pigs 
      experimentally infected with an attenuated PRRSV strain at 46 days post 
      infection. A total of 6404 differentially expressed genes (DEGs) were detected of 
      which 3960 DEGs were upregulated and 2444 DEGs were downregulated. Specifically, 
      genes involved in innate immune responses and chemokines and receptors associated 
      with T-cell homing to lymphoid tissues were down regulated. As a result, homing 
      of virus-specific T-cells to lymphoid tissues seems to be ineffective, evidenced 
      by the lower frequencies of virus-specific T-cell in lymphoid tissue than in 
      peripheral blood. Genes associated with T-cell exhaustion were upregulated. 
      Likewise, genes involved in the anti-apoptotic pathway were upregulated. 
      Collectively, the data suggested that the live-attenuated PRRSV strain 
      establishes a pro-survival microenvironment in lymphoid tissue by suppressing 
      innate immune responses, T-cell homing, and preventing cell apoptosis.
FAU - Chaudhari, Jayeshbhai
AU  - Chaudhari J
AUID- ORCID: 0000-0002-1864-562X
AD  - Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, 
      USA.
AD  - School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, Lincoln, NE 68583, USA.
FAU - Liew, Chia-Sin
AU  - Liew CS
AD  - Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
FAU - Workman, Aspen M
AU  - Workman AM
AUID- ORCID: 0000-0003-2852-3519
AD  - USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE 68933, USA.
FAU - Riethoven, Jean-Jack M
AU  - Riethoven JM
AUID- ORCID: 0000-0002-2709-7880
AD  - Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
FAU - Steffen, David
AU  - Steffen D
AUID- ORCID: 0000-0002-5166-2296
AD  - School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, Lincoln, NE 68583, USA.
FAU - Sillman, Sarah
AU  - Sillman S
AD  - School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, Lincoln, NE 68583, USA.
FAU - Vu, Hiep L X
AU  - Vu HLX
AUID- ORCID: 0000-0001-8084-4358
AD  - Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, 
      USA.
AD  - Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583, 
      USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20200728
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics/immunology
MH  - *Gene Expression Profiling
MH  - Host Microbial Interactions/*genetics/*immunology
MH  - Immunity, Innate/*genetics
MH  - Lymphoid Tissue/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/immunology
MH  - Swine/virology
MH  - T-Lymphocytes/immunology
PMC - PMC7474429
OTO - NOTNLM
OT  - PRRSV
OT  - RNA-sequencing
OT  - T-cell exhaustion
OT  - apoptosis
OT  - persistent infection
OT  - transcriptome
COIS- H.L.X.V. is named on the United States patent No. 10738088 entitled "A 
      non-naturally occuring porcine reproductive and respiratory syndrome virus 
      (prrsv) and methods of using". The other authors declare no conflict of interest.
EDAT- 2020/08/01 06:00
MHDA- 2021/02/25 06:00
PMCR- 2020/08/01
CRDT- 2020/08/01 06:00
PHST- 2020/06/24 00:00 [received]
PHST- 2020/07/15 00:00 [revised]
PHST- 2020/07/24 00:00 [accepted]
PHST- 2020/08/01 06:00 [entrez]
PHST- 2020/08/01 06:00 [pubmed]
PHST- 2021/02/25 06:00 [medline]
PHST- 2020/08/01 00:00 [pmc-release]
AID - v12080817 [pii]
AID - viruses-12-00817 [pii]
AID - 10.3390/v12080817 [doi]
PST - epublish
SO  - Viruses. 2020 Jul 28;12(8):817. doi: 10.3390/v12080817.