PMID- 32733511
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 11
DP  - 2020
TI  - Copper-Induced Activation of MAPKs, CDPKs and CaMKs Triggers Activation of 
      Hexokinase and Inhibition of Pyruvate Kinase Leading to Increased Synthesis of 
      ASC, GSH and NADPH in Ulva compressa.
PG  - 990
LID - 10.3389/fpls.2020.00990 [doi]
LID - 990
AB  - In order to analyze whether copper induces activation of CaMK, CDPK and/or MAPK 
      signaling pathways leading to carbon flux reprogramming and to the synthesis of 
      ascorbate (ASC), glutathione (GSH) and NADPH in order to buffer copper-induced 
      oxidative stress, U. compressa was initially cultivated with 10 microM copper for 0 
      to 10 days. The activities of hexokinase (HK), pyruvate kinase (PK), L-galactone 
      1,4 lactone dehydrogenase (L-GLDH) and glucose 6-P dehydrogenase (G6PDH) were 
      analyzed. HK activity was increased whereas PK was inhibited, and L-GLDH and 
      G6PDH activities were increased indicating a copper-induced modulation of 
      glycolysis leading to carbon flux reprogramming. Then, the alga was cultivated 
      with an inhibitor of CaMs and CaMKs, CDPKs and MAPKs, and with 10 microM of copper 
      for 5 days and the activities of HK, PK, L-GLDH, G6PDH and glutathione synthase 
      (GS), the levels of ASC/DHA, GSG/GSSG and NADPH/NADP, the levels of superoxide 
      anions (SA) and hydrogen peroxide (HP) and the integrity of plasma membrane were 
      determined. The activation of HK was dependent on MAPKs, the inhibition of PK on 
      CDPKs/MAPKs, the activation of L-GLDH on MAPKs, the activation GS on CDPKs/MAPKs, 
      and the activation of G6PDH on MAPKs. Increases in the level of ASC/DHA were 
      dependent on activation of CaMKs/CDPKs/MAPKs, those of GSG/GSSG on MAPKs and 
      those NADPH/NADP on CaMKs/CDPKs/MAPKs. The accumulation of superoxide anions and 
      hydrogen peroxide and the integrity of plasma membrane were dependent on 
      CaMKs/CDPKs/MAPKs. Thus, copper induced the activation of MAPKs, CDPKs and CaMKs 
      leading to the modulation of glycolysis and carbon flux reprogramming which 
      trigger an increase in ASC, GSH and NADPH syntheses and the activation of 
      antioxidant enzymes in order to buffer copper-induced oxidative stress in U. 
      compressa.
CI  - Copyright (c) 2020 Laporte, Gonzalez and Moenne.
FAU - Laporte, Daniel
AU  - Laporte D
AD  - Laboratory of Marine Biotechnology, Faculty of Chemistry and Biology, University 
      of Santiago of Chile, Santiago, Chile.
FAU - Gonzalez, Alberto
AU  - Gonzalez A
AD  - Laboratory of Marine Biotechnology, Faculty of Chemistry and Biology, University 
      of Santiago of Chile, Santiago, Chile.
FAU - Moenne, Alejandra
AU  - Moenne A
AD  - Laboratory of Marine Biotechnology, Faculty of Chemistry and Biology, University 
      of Santiago of Chile, Santiago, Chile.
LA  - eng
PT  - Journal Article
DEP - 20200709
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC7363978
OTO - NOTNLM
OT  - CDPKs
OT  - CaMKs
OT  - MAPKs
OT  - NADPH
OT  - Ulva compressa
OT  - ascorbate
OT  - copper
OT  - glutathione
EDAT- 2020/08/01 06:00
MHDA- 2020/08/01 06:01
PMCR- 2020/01/01
CRDT- 2020/08/01 06:00
PHST- 2020/04/16 00:00 [received]
PHST- 2020/06/17 00:00 [accepted]
PHST- 2020/08/01 06:00 [entrez]
PHST- 2020/08/01 06:00 [pubmed]
PHST- 2020/08/01 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2020.00990 [doi]
PST - epublish
SO  - Front Plant Sci. 2020 Jul 9;11:990. doi: 10.3389/fpls.2020.00990. eCollection 
      2020.