PMID- 32733887
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 8
DP  - 2020
TI  - Maternal Cytokines CXCL12, VEGFA, and WNT5A Promote Porcine Oocyte Maturation via 
      MAPK Activation and Canonical WNT Inhibition.
PG  - 578
LID - 10.3389/fcell.2020.00578 [doi]
LID - 578
AB  - Maternal regulatory factors endow the oocyte with developmental competence in 
      vivo, which might be absent in current in vitro maturation (IVM) systems, thereby 
      compromising oocyte quality. In the present study, by employing RNA sequencing 
      data analysis, we expect to identify potential contributing factors to support 
      porcine oocyte maturation through binding to their receptors on the oolemma. 
      Here, C-X-C motif chemokine ligand 12 (CXCL12), vascular endothelial growth 
      factor A (VEGFA), and Wingless-type MMTV integration site family member 5A 
      (WNT5A), termed CVW, are selected and confirmed to be important maternal 
      cytokines for porcine oocyte maturation. Combined supplementation of CVW promotes 
      the nuclear maturation percentage from 57.2% in controls to 75.9%. More 
      importantly, these maternal cytokines improve the developmental potential of 
      matured oocytes by parthenogenesis, fertilization, and cloning, as their 
      blastocyst formation efficiencies and total cell numbers are increased. CVW 
      supplementation also enlarges perivitelline space and promotes cumulus expansion, 
      which results in a more complete transzonal projection retraction on the zona 
      pellucida, and a reduced incidence of polyspermy in fertilized oocytes. 
      Meanwhile, inhibiting the CVW receptor-mediated signaling pathways severely 
      impairs oocyte meiotic resumption and cumulus expansion during IVM. We further 
      determine that maturation improvement by CVW is achieved through activating the 
      MAPK pathway in advance and inhibiting the canonical WNT pathway at the end of 
      the IVM period. These findings provide a new combination of three cytokines to 
      promote the porcine IVM process, which also holds potential to be used in human 
      assisted reproduction technologies as well as in other species.
CI  - Copyright (c) 2020 Liu, Hao, Li, Zhou, Zhu, Bu, Liu, Hou, Zhang and Miao.
FAU - Liu, Xin
AU  - Liu X
AD  - Institute of Stem Cell and Regenerative Biology, College of Animal Science and 
      Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction 
      (Huazhong Agricultural University), Ministry of Education, Wuhan, China.
FAU - Hao, Yuchen
AU  - Hao Y
AD  - Institute of Stem Cell and Regenerative Biology, College of Animal Science and 
      Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction 
      (Huazhong Agricultural University), Ministry of Education, Wuhan, China.
FAU - Li, Zhekun
AU  - Li Z
AD  - Institute of Stem Cell and Regenerative Biology, College of Animal Science and 
      Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction 
      (Huazhong Agricultural University), Ministry of Education, Wuhan, China.
FAU - Zhou, Jilong
AU  - Zhou J
AD  - Institute of Stem Cell and Regenerative Biology, College of Animal Science and 
      Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction 
      (Huazhong Agricultural University), Ministry of Education, Wuhan, China.
FAU - Zhu, Hongmei
AU  - Zhu H
AD  - College of Animal Science and Veterinary Medicine, Huazhong Agricultural 
      University, Wuhan, China.
FAU - Bu, Guowei
AU  - Bu G
AD  - Institute of Stem Cell and Regenerative Biology, College of Animal Science and 
      Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction 
      (Huazhong Agricultural University), Ministry of Education, Wuhan, China.
FAU - Liu, Zhiting
AU  - Liu Z
AD  - Institute of Stem Cell and Regenerative Biology, College of Animal Science and 
      Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction 
      (Huazhong Agricultural University), Ministry of Education, Wuhan, China.
FAU - Hou, Xudong
AU  - Hou X
AD  - College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 
      China.
FAU - Zhang, Xia
AU  - Zhang X
AD  - College of Animal Science and Veterinary Medicine, Huazhong Agricultural 
      University, Wuhan, China.
FAU - Miao, Yi-Liang
AU  - Miao YL
AD  - Institute of Stem Cell and Regenerative Biology, College of Animal Science and 
      Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction 
      (Huazhong Agricultural University), Ministry of Education, Wuhan, China.
AD  - The Cooperative Innovation Center for Sustainable Pig Production, Huazhong 
      Agricultural University, Wuhan, China.
LA  - eng
PT  - Journal Article
DEP - 20200707
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC7358312
OTO - NOTNLM
OT  - CXCL12
OT  - VEGFA
OT  - WNT5A
OT  - embryo development
OT  - in vitro maturation
OT  - porcine oocyte
EDAT- 2020/08/01 06:00
MHDA- 2020/08/01 06:01
PMCR- 2020/01/01
CRDT- 2020/08/01 06:00
PHST- 2020/04/01 00:00 [received]
PHST- 2020/06/15 00:00 [accepted]
PHST- 2020/08/01 06:00 [entrez]
PHST- 2020/08/01 06:00 [pubmed]
PHST- 2020/08/01 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fcell.2020.00578 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2020 Jul 7;8:578. doi: 10.3389/fcell.2020.00578. eCollection 
      2020.