PMID- 32735097
OWN - NLM
STAT- MEDLINE
DCOM- 20201102
LR  - 20201102
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 21
IP  - 4
DP  - 2020 Jul
TI  - Comparison of the presentation of atopic dermatitis induced by 
      trinitrochlorobenzene and house dust mite in NC/Nga mice.
PG  - e59
LID - 10.4142/jvs.2020.21.e59 [doi]
LID - e59
AB  - BACKGROUND: Atopic dermatitis (AD) is a common chronic inflammatory skin disease. 
      To understand AD, there have been many trials establishing AD animal models. 
      Although various trials to establish AD animal models have been existed, even the 
      mechanisms of AD in animal models are not enough clarified. OBJECTIVES: This 
      study assessed AD characteristics induced in Nishiki-nezumi Cinnamon/Nagoya 
      (Nc/Nga) mice following trinitrochlorobenzene (TNCB) treatment for different 
      periods and house dust mite (HDM) treatment to compare each model's immunological 
      patterns, especially with cytokine antibody array tool. METHODS: In this study, 
      we exposed Nc/Nga mice to TNCB or HDM extract to induce AD. Nc/Nga mice were 
      divided into 4 groups: control, TNCB 2 weeks-treated, TNCB 8 weeks-treated, and 
      HDM-treated groups. After AD induction, all mice were evaluated by serum 
      immunoglobulin E (IgE) concentration and serum cytokine antibody assays, scoring 
      of skin lesions, scoring of scratching frequency, and histological analysis. 
      RESULTS: The results showed significant differences between groups in serum IgE 
      concentration, skin lesion scores, and scratching frequency. The analysis results 
      for serum cytokine antibody arrays showed that in the TNCB 8 weeks- and 
      HDM-treated groups, but not in the TNCB 2 weeks-treated group, expressions of 
      genes related to the immune response were enriched. Among the histological 
      results, the skin lesions in the HDM-treated group were most similar to those of 
      AD. CONCLUSIONS: We confirmed that immunological pattern of AD mice was markedly 
      different between HDM and TNCB treated groups. In addition, the immunological 
      pattern was quietly different dependent on TNCB treated duration.
CI  - (c) 2020 The Korean Society of Veterinary Science.
FAU - Kim, Yoon Hwan
AU  - Kim YH
AUID- ORCID: 0000-0002-0727-9927
AD  - Department of Veterinary Internal Medicine and Institute of Veterinary Science, 
      College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, 
      Korea.
FAU - Kim, Tae Hyeong
AU  - Kim TH
AUID- ORCID: 0000-0002-0938-0818
AD  - Department of Anatomy and Institute of Veterinary Science, College of Veterinary 
      Medicine, Kangwon National University, Chuncheon 24341, Korea.
FAU - Kang, Min Soo
AU  - Kang MS
AUID- ORCID: 0000-0002-1698-9445
AD  - Department of Anatomy and Institute of Veterinary Science, College of Veterinary 
      Medicine, Kangwon National University, Chuncheon 24341, Korea.
FAU - Ahn, Jin Ok
AU  - Ahn JO
AUID- ORCID: 0000-0002-3300-6084
AD  - Department of Veterinary Internal Medicine and Institute of Veterinary Science, 
      College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, 
      Korea.
FAU - Choi, Jung Hoon
AU  - Choi JH
AUID- ORCID: 0000-0002-3725-4907
AD  - Department of Anatomy and Institute of Veterinary Science, College of Veterinary 
      Medicine, Kangwon National University, Chuncheon 24341, Korea. 
      jhchoi@kangwon.ac.kr.
FAU - Chung, Jin Young
AU  - Chung JY
AUID- ORCID: 0000-0001-6729-9834
AD  - Department of Veterinary Internal Medicine and Institute of Veterinary Science, 
      College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, 
      Korea. chungjinyoung@kangwon.ac.kr.
LA  - eng
GR  - PJ01395602/RDA/Rural Development Administration/Korea
PT  - Journal Article
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Cytokines)
RN  - Z4ZG7O5SZ9 (Picryl Chloride)
SB  - IM
MH  - Animals
MH  - Cytokines/*analysis
MH  - Dermatitis, Atopic/chemically induced/*immunology/parasitology
MH  - Disease Models, Animal
MH  - Female
MH  - Mice
MH  - Picryl Chloride/*adverse effects
MH  - Pyroglyphidae/*physiology
MH  - Time Factors
PMC - PMC7402931
OTO - NOTNLM
OT  - Atopic dermatitis
OT  - Nc/Nga mouse
OT  - cytokines
OT  - house dust mite
OT  - trinitrochlorobenzene
COIS- The authors declare no conflicts of interest.
EDAT- 2020/08/01 06:00
MHDA- 2020/11/03 06:00
PMCR- 2020/07/01
CRDT- 2020/08/01 06:00
PHST- 2020/01/17 00:00 [received]
PHST- 2020/04/01 00:00 [revised]
PHST- 2020/04/12 00:00 [accepted]
PHST- 2020/08/01 06:00 [entrez]
PHST- 2020/08/01 06:00 [pubmed]
PHST- 2020/11/03 06:00 [medline]
PHST- 2020/07/01 00:00 [pmc-release]
AID - 21.e59 [pii]
AID - 10.4142/jvs.2020.21.e59 [doi]
PST - ppublish
SO  - J Vet Sci. 2020 Jul;21(4):e59. doi: 10.4142/jvs.2020.21.e59.