PMID- 32735103
OWN - NLM
STAT- MEDLINE
DCOM- 20201102
LR  - 20201102
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 21
IP  - 4
DP  - 2020 Jul
TI  - A rapid and quantitative fluorescent microsphere immunochromatographic strip test 
      for detection of antibodies to porcine reproductive and respiratory syndrome 
      virus.
PG  - e68
LID - 10.4142/jvs.2020.21.e68 [doi]
LID - e68
AB  - A fluorescent microsphere-based immunochromatographic strip test (FICT) was 
      developed for the rapid, sensitive, and quantitative detection of porcine 
      reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. 
      The assay was based on the formation of a sandwich immune-complex (anti-pig 
      IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with 
      IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, 
      sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. 
      FICT showed a good correlation with the virus neutralization assay. Overall, a 
      promising pen-side diagnostic tool was developed for the rapid and quantitative 
      detection of PRRSV antibodies within 15 min.
CI  - (c) 2020 The Korean Society of Veterinary Science.
FAU - Wei, Yanqiu
AU  - Wei Y
AUID- ORCID: 0000-0001-8306-041X
AD  - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of 
      Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Yang, Baozhi
AU  - Yang B
AUID- ORCID: 0000-0003-4354-5935
AD  - College of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning 
      530004, China.
FAU - Li, Yunlong
AU  - Li Y
AUID- ORCID: 0000-0001-6005-2150
AD  - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of 
      Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Duan, Yongcheng
AU  - Duan Y
AUID- ORCID: 0000-0003-0877-6447
AD  - College of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning 
      530004, China.
FAU - Tian, Deyu
AU  - Tian D
AUID- ORCID: 0000-0002-6565-3323
AD  - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of 
      Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - He, Baoxiang
AU  - He B
AUID- ORCID: 0000-0002-2040-2651
AD  - College of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning 
      530004, China.
FAU - Chen, Chuangfu
AU  - Chen C
AUID- ORCID: 0000-0002-9222-5260
AD  - College of Animal Science and Technology, Shihezi University, Shihezi 832003, 
      China.
FAU - Liu, Wenjun
AU  - Liu W
AUID- ORCID: 0000-0002-3080-8655
AD  - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of 
      Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
AD  - University of Chinese Academy of Sciences, Beijing 100049, China.
FAU - Yang, Limin
AU  - Yang L
AUID- ORCID: 0000-0002-1158-0800
AD  - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of 
      Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
AD  - College of Animal Science and Technology, Shihezi University, Shihezi 832003, 
      China. lmyang@im.ac.cn.
LA  - eng
GR  - 2018YFC1200500/National Key Technologies Research and Development Program of 
      China/China
GR  - 2018YFC1200600/National Key Technologies Research and Development Program of 
      China/China
GR  - 2016ZX10004222-006/National Key Program for Infectious Diseases of China/China
GR  - 81621091/NSFC/National Natural Science Foundation of China/China
PT  - Journal Article
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Antibodies, Viral)
RN  - 0 (Reagent Strips)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*immunology
MH  - Chromatography, Affinity/methods/*veterinary
MH  - Enzyme-Linked Immunosorbent Assay/methods/*veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/immunology
MH  - Porcine respiratory and reproductive syndrome virus/immunology/*isolation & 
      purification
MH  - Reagent Strips/therapeutic use
MH  - Swine
PMC - PMC7402939
OTO - NOTNLM
OT  - Fluorescence
OT  - PRRSV
OT  - immunochromatographic assay
OT  - immunochromatography
OT  - pen-side detection
COIS- The authors declare no conflicts of interest.
EDAT- 2020/08/01 06:00
MHDA- 2020/11/03 06:00
PMCR- 2020/07/01
CRDT- 2020/08/01 06:00
PHST- 2019/11/14 00:00 [received]
PHST- 2020/04/08 00:00 [revised]
PHST- 2020/04/09 00:00 [accepted]
PHST- 2020/08/01 06:00 [entrez]
PHST- 2020/08/01 06:00 [pubmed]
PHST- 2020/11/03 06:00 [medline]
PHST- 2020/07/01 00:00 [pmc-release]
AID - 21.e68 [pii]
AID - 10.4142/jvs.2020.21.e68 [doi]
PST - ppublish
SO  - J Vet Sci. 2020 Jul;21(4):e68. doi: 10.4142/jvs.2020.21.e68.