PMID- 32736675
OWN - NLM
STAT- MEDLINE
DCOM- 20210222
LR  - 20211204
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 529
IP  - 3
DP  - 2020 Aug 27
TI  - NCOA5 is a master regulator of amino acid-induced mTOR activation and beta-casein 
      synthesis in bovine mammary epithelial cells.
PG  - 569-574
LID - S0006-291X(20)31131-1 [pii]
LID - 10.1016/j.bbrc.2020.05.193 [doi]
AB  - The nuclear receptor co-activator 5 (NCOA5) is known as a co-activator or 
      co-repressor that influences gene expression and cellular physiology, but its 
      roles and detailed molecular mechanism is still largely unknown. In this study, 
      we explored the role and molecular mechanism of NCOA5 in amino acid-induced 
      activation of the mechanistic target of rapamycin (mTOR) and milk protein 
      synthesis in bovine mammary epithelial cells (BMECs). Methionine (Met) and 
      leucine (Leu) significantly up-regulated the expression of NCOA5. NCOA5 
      overexpression increased mTOR phosphorylation and beta-casein synthesis, whereas its 
      knockdown exhibited the opposite effects. Furthermore, inhibition of 
      phosphatidylinositol 3-kinase (PI3K) completely abolished the stimulatory effects 
      of Met and Leu on NCOA5 expression. ChIP-qPCR analysis detected that NCOA5 bound 
      to the mTOR promoter, and this interaction was enhanced by the stimulation of Met 
      and Leu. These above data reveal that NCOA5 is a key regulator of amino 
      acid-induced PI3K-mediated mTOR activation and beta-casein synthesis in BMECs.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Yuan, Xiaohan
AU  - Yuan X
AD  - The Key Laboratory of Dairy Science of Education Ministry, College of Life 
      Science, Northeast Agricultural University, Harbin, China.
FAU - Zhang, Li
AU  - Zhang L
AD  - The Key Laboratory of Dairy Science of Education Ministry, College of Life 
      Science, Northeast Agricultural University, Harbin, China.
FAU - Cui, Yingjun
AU  - Cui Y
AD  - The Key Laboratory of Dairy Science of Education Ministry, College of Life 
      Science, Northeast Agricultural University, Harbin, China.
FAU - Yu, Yanbo
AU  - Yu Y
AD  - The Key Laboratory of Dairy Science of Education Ministry, College of Life 
      Science, Northeast Agricultural University, Harbin, China.
FAU - Gao, Xuejun
AU  - Gao X
AD  - School of Animal Science, Yangtze University, Jingzhou, China.
FAU - Ao, Jinxia
AU  - Ao J
AD  - The Key Laboratory of Dairy Science of Education Ministry, College of Life 
      Science, Northeast Agricultural University, Harbin, China. Electronic address: 
      ajx050723@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200715
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Amino Acids)
RN  - 0 (Caseins)
RN  - 0 (Nuclear Receptor Coactivators)
RN  - AE28F7PNPL (Methionine)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Amino Acids/*pharmacology
MH  - Animals
MH  - Caseins/*metabolism
MH  - Cattle
MH  - Cells, Cultured
MH  - Enzyme Activation/drug effects
MH  - Epithelial Cells/*drug effects/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Leucine/pharmacology
MH  - Mammary Glands, Animal/cytology/metabolism
MH  - Methionine/pharmacology
MH  - Nuclear Receptor Coactivators/genetics/*metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
OTO - NOTNLM
OT  - Amino acid
OT  - Bovine mammary epithelial cells
OT  - NCOA5
OT  - mTOR
OT  - beta-casein
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/08/02 06:00
MHDA- 2021/02/23 06:00
CRDT- 2020/08/02 06:00
PHST- 2020/05/12 00:00 [received]
PHST- 2020/05/25 00:00 [accepted]
PHST- 2020/08/02 06:00 [entrez]
PHST- 2020/08/02 06:00 [pubmed]
PHST- 2021/02/23 06:00 [medline]
AID - S0006-291X(20)31131-1 [pii]
AID - 10.1016/j.bbrc.2020.05.193 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2020 Aug 27;529(3):569-574. doi: 
      10.1016/j.bbrc.2020.05.193. Epub 2020 Jul 15.