PMID- 32736961
OWN - NLM
STAT- MEDLINE
DCOM- 20220131
LR  - 20220131
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 145
DP  - 2021 Sep
TI  - Dendritic cell engineered cTXN as new vaccine prospect against L. donovani.
PG  - 155208
LID - S1043-4666(20)30224-6 [pii]
LID - 10.1016/j.cyto.2020.155208 [doi]
AB  - Dendritic cells (DCs), as antigen-presenting cells, can reportedly be infected 
      withLeishmaniaparasites and hence provide a better option to trigger T-cell 
      primary immune responses and immunological memory. We consistently primed DCs 
      during culture with purified recombinant cytosolic tryparedoxin (rcTXN) and then 
      evaluated the vaccine prospect of presentation of rcTXN against VL in BALB/c 
      mice. We reported earlier the immunogenic properties of cTXN antigen derived 
      fromL. donovani when anti-cTXN antibody was detected in the sera of kala-azar 
      patients. It was observed that cTXN antigen, when used as an immunogen with 
      murine DCs acting as a vehicle, was able to induce complete protection against VL 
      in an infected group of immunized mice. This vaccination triggered splenic 
      macrophages to produce more IL-12 and GM-CSF, and restricted IL-10 release to a 
      minimum in an immunized group of infected animals. Concomitant changes in T-cell 
      responses against cTXN antigen were also noticed, which increased the release of 
      protective cytokine-like IFN-gamma under the influence of NF-kappabeta in the indicated 
      vaccinated group of animals. All cTXN-DCs-vaccinated BALB/c mice survived during 
      the experimental period of 120 days. The results obtained in our study suggest 
      that DCs primed with cTXN can be used as a vaccine prospect for the control of 
      visceral leishmaniasis.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Suman, Shashi S
AU  - Suman SS
AD  - Department of Immunology, Rajendra Memorial Research Institute of Medical 
      Sciences, Patna 800007, India.
FAU - Kumar, Akhilesh
AU  - Kumar A
AD  - Department of Immunology, Rajendra Memorial Research Institute of Medical 
      Sciences, Patna 800007, India.
FAU - Singh, Ashish K
AU  - Singh AK
AD  - Department of Immunology, Rajendra Memorial Research Institute of Medical 
      Sciences, Patna 800007, India.
FAU - Amit, Ajay
AU  - Amit A
AD  - Department of Forensic Science, Guru Ghasidas Vishwavidyalaya, Bilaspur (C.G.) 
      495009, India.
FAU - Topno, R K
AU  - Topno RK
AD  - Department of Epidemiology, Rajendra Memorial Research Institute of Medical 
      Sciences, Patna 800007, India.
FAU - Pandey, K
AU  - Pandey K
AD  - Department of Clinical Medicine, Rajendra Memorial Research Institute of Medical 
      Sciences, Patna 800007, India.
FAU - Das, V N R
AU  - Das VNR
AD  - Department of Clinical Medicine, Rajendra Memorial Research Institute of Medical 
      Sciences, Patna 800007, India.
FAU - Das, P
AU  - Das P
AD  - Department of Molecular Biology, Rajendra Memorial Research Institute of Medical 
      Sciences, Patna 800007, India.
FAU - Ali, Vahab
AU  - Ali V
AD  - Department of Biochemistry, Rajendra Memorial Research Institute of Medical 
      Sciences, Patna 800007, India.
FAU - Bimal, Sanjiva
AU  - Bimal S
AD  - Department of Immunology, Rajendra Memorial Research Institute of Medical 
      Sciences, Patna 800007, India. Electronic address: drsbimal24@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20200728
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Cytokines)
RN  - 0 (Leishmaniasis Vaccines)
RN  - 0 (Protozoan Proteins)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Cytokines/immunology
MH  - Dendritic Cells/*immunology/parasitology
MH  - Immunity, Cellular/immunology
MH  - Interleukin-10/immunology
MH  - Interleukin-12/immunology
MH  - Leishmania donovani/*immunology
MH  - Leishmaniasis Vaccines/*immunology
MH  - Leishmaniasis, Visceral/*immunology/parasitology
MH  - Macrophages/immunology/parasitology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Protozoan Proteins/immunology
MH  - T-Lymphocytes/immunology/parasitology
OTO - NOTNLM
OT  - Dendritic cells
OT  - GM-CSF
OT  - IFN-gamma
OT  - IL-10
OT  - Macrophages
OT  - NFkappabeta
OT  - Vaccine
OT  - Visceral leishmaniasis
OT  - cTXN
EDAT- 2020/08/02 06:00
MHDA- 2022/02/01 06:00
CRDT- 2020/08/02 06:00
PHST- 2020/01/14 00:00 [received]
PHST- 2020/07/06 00:00 [revised]
PHST- 2020/07/11 00:00 [accepted]
PHST- 2020/08/02 06:00 [pubmed]
PHST- 2022/02/01 06:00 [medline]
PHST- 2020/08/02 06:00 [entrez]
AID - S1043-4666(20)30224-6 [pii]
AID - 10.1016/j.cyto.2020.155208 [doi]
PST - ppublish
SO  - Cytokine. 2021 Sep;145:155208. doi: 10.1016/j.cyto.2020.155208. Epub 2020 Jul 28.