PMID- 32737916
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1529-8019 (Electronic)
IS  - 1396-0296 (Linking)
VI  - 33
IP  - 6
DP  - 2020 Nov
TI  - Genetically modified adipose-derived stem cells with matrix metalloproteinase 3 
      promote scarless cutaneous repair.
PG  - e14112
LID - 10.1111/dth.14112 [doi]
AB  - Adipose-derived stem cells (ASCs) possess strong regenerative potencies and have 
      been used to improve wound healing in animal models and clinical studies. 
      However, the use of ASCs on scarless wound healing is not satisfactory. Matrix 
      metalloproteinase 3 (MMP-3) is involved in extracellular matrix (ECM) remolding 
      and scar formation. We aimed to investigate the effect of ASCs stable expressing 
      MMP-3 (ASCs-MMP-3) on wound healing and scarring. A cutaneous wound healing 
      animal model was used to assess the effect of ASCs and ASCs-MMP-3 on wound 
      healing and scar formation. The target protein levels in the wound tissues were 
      determined by western blot assay. Our results demonstrated that ASCs alone 
      promoted wound healing but had a negligible effect on reducing scarring. 
      ASCs-MMP-3 not only possessed the ability of ASCs to speed up wound healing, but 
      also incorporated the capability of MMP-3 to reduce scaring. Overexpressing of 
      MMP-3 decreased the collagen I, transforming growth factor (TGF)-beta1, and alpha-smooth 
      muscle actin (alpha-SMA) levels and enhanced collagen III and TGF-beta3 levels which 
      contributed to reducing scar formation. Our studies suggested that ASCs-MMP-3 is 
      a potential candidate for developing effective therapeutic strategies for 
      scarless wound healing.
CI  - (c) 2020 Wiley Periodicals LLC.
FAU - Rong, Shouxiang
AU  - Rong S
AUID- ORCID: 0000-0002-9464-1305
AD  - Department of Plastic Surgery, Caoxian Hospital of Traditional Chinese Medicine, 
      Heze City, Shandong Province, China.
AD  - Department of Plastic Surgery, Sanya People's Hospital, Sanya, Hainan Province, 
      China.
FAU - Li, Chunlan
AU  - Li C
AD  - Department of Plastic Surgery, Sanya People's Hospital, Sanya, Hainan Province, 
      China.
FAU - Li, Shuting
AU  - Li S
AD  - Department of Plastic Surgery, Sanya People's Hospital, Sanya, Hainan Province, 
      China.
FAU - Wu, Shaoqiang
AU  - Wu S
AD  - Department of Plastic Surgery, Sanya People's Hospital, Sanya, Hainan Province, 
      China.
FAU - Sun, Fei
AU  - Sun F
AD  - Department of Plastic Surgery, Sanya People's Hospital, Sanya, Hainan Province, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20200827
PL  - United States
TA  - Dermatol Ther
JT  - Dermatologic therapy
JID - 9700070
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adipocytes
MH  - Adipose Tissue
MH  - Animals
MH  - Cicatrix/pathology/prevention & control
MH  - *Matrix Metalloproteinase 3/genetics
MH  - *Stem Cells/pathology
MH  - Wound Healing
OTO - NOTNLM
OT  - ASCs
OT  - MMP-3
OT  - TGF-beta
OT  - collagens
OT  - scarless wound healing
EDAT- 2020/08/02 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/08/02 06:00
PHST- 2020/06/24 00:00 [received]
PHST- 2020/07/24 00:00 [revised]
PHST- 2020/07/28 00:00 [accepted]
PHST- 2020/08/02 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/08/02 06:00 [entrez]
AID - 10.1111/dth.14112 [doi]
PST - ppublish
SO  - Dermatol Ther. 2020 Nov;33(6):e14112. doi: 10.1111/dth.14112. Epub 2020 Aug 27.