PMID- 32738527
OWN - NLM
STAT- MEDLINE
DCOM- 20210322
LR  - 20210322
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1153
DP  - 2020 Sep 15
TI  - The use of ultra-high-pressure-liquid-chromatography tandem time-of-flight mass 
      spectrometry as a confirmatory method in drug residue analysis: Application to 
      the determination of antibiotics in piglet liver.
PG  - 122264
LID - S1570-0232(20)30432-3 [pii]
LID - 10.1016/j.jchromb.2020.122264 [doi]
AB  - Consumption of food containing antibiotic residues can cause direct toxic 
      effects, such as allergic reactions, or indirect effects, like the possibility to 
      contribute to the development of antimicrobial resistance. It is essential for 
      consumer food safety, as well as for public health, to monitor antibiotics in 
      foods of animal origin intended for human consumption, particularly in piglets, a 
      highly consumed meat in Portugal. Concerning the lack of regulations regarding 
      the validation criteria for the ToF-MS (time-of-flight mass spectrometry) drug 
      residues determination, this study presents an approach for the use of UHPLC 
      (ultra-high-pressure-liquid-chromatography)-ToF-MS not only for screening but 
      also for confirmatory purposes of 45 antibiotics from 7 different classes. Thus, 
      a method was optimized and validated in accordance with Decision 2002/657/EC by 
      evaluating CCalpha, CCbeta, specificity, selectivity, recovery, ruggedness, 
      repeatability and reproducibility. For antibiotics with no MRL set, the lowest 
      values achieved for CCalpha and CCbeta were, respectively, of 0.1 mug kg(-1) and 
      0.2 mug kg(-1) for cinoxacin. Regarding precision, the CV values obtained ranged 
      from 5% to 21% for repeatability and from 6% to 22% for reproducibility. 
      Recoveries were calculated falling between 82% and 120%. After validation, the 
      method was used to analyse piglet liver samples. Antibiotic residues were 
      determined in 47% of the 21 analysed samples. Nevertheless, all the antibiotics 
      were detected at concentrations below the MRLs.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Magalhaes, Daniela
AU  - Magalhaes D
AD  - University of Coimbra, Pharmacy Faculty, Azinhaga de Santa Comba, 3000-548 
      Coimbra, Portugal.
FAU - Freitas, Andreia
AU  - Freitas A
AD  - INIAV - Instituto Nacional de Investigacao Agraria e Veterinaria, I.P., Rua dos 
      Lagidos, Lugar da Madalena, 4485-655 Vairao, Vila do Conde, Portugal; 
      REQUIMTE/LAQV, University of Oporto, 4051-401 Porto, Portugal.
FAU - Sofia Vila Pouca, Ana
AU  - Sofia Vila Pouca A
AD  - INIAV - Instituto Nacional de Investigacao Agraria e Veterinaria, I.P., Rua dos 
      Lagidos, Lugar da Madalena, 4485-655 Vairao, Vila do Conde, Portugal.
FAU - Barbosa, Jorge
AU  - Barbosa J
AD  - INIAV - Instituto Nacional de Investigacao Agraria e Veterinaria, I.P., Rua dos 
      Lagidos, Lugar da Madalena, 4485-655 Vairao, Vila do Conde, Portugal; 
      REQUIMTE/LAQV, University of Oporto, 4051-401 Porto, Portugal.
FAU - Ramos, Fernando
AU  - Ramos F
AD  - University of Coimbra, Pharmacy Faculty, Azinhaga de Santa Comba, 3000-548 
      Coimbra, Portugal; REQUIMTE/LAQV, University of Oporto, 4051-401 Porto, Portugal. 
      Electronic address: fjramos@ci.uc.pt.
LA  - eng
PT  - Journal Article
DEP - 20200713
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drug Residues/*analysis
MH  - Food Safety
MH  - Limit of Detection
MH  - Liver/*chemistry
MH  - Mass Spectrometry/*methods
MH  - Meat/analysis
MH  - Reproducibility of Results
MH  - Swine
OTO - NOTNLM
OT  - Antibiotics
OT  - Food safety
OT  - Piglet liver
OT  - UHPLC-ToF-MS
COIS- Declaration of Competing Interest None.
EDAT- 2020/08/02 06:00
MHDA- 2021/03/23 06:00
CRDT- 2020/08/02 06:00
PHST- 2020/03/20 00:00 [received]
PHST- 2020/06/02 00:00 [revised]
PHST- 2020/07/08 00:00 [accepted]
PHST- 2020/08/02 06:00 [pubmed]
PHST- 2021/03/23 06:00 [medline]
PHST- 2020/08/02 06:00 [entrez]
AID - S1570-0232(20)30432-3 [pii]
AID - 10.1016/j.jchromb.2020.122264 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Sep 15;1153:122264. doi: 
      10.1016/j.jchromb.2020.122264. Epub 2020 Jul 13.