PMID- 32742479
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201006
IS  - 1837-9664 (Print)
IS  - 1837-9664 (Electronic)
IS  - 1837-9664 (Linking)
VI  - 11
IP  - 18
DP  - 2020
TI  - Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous 
      Cell Carcinoma.
PG  - 5329-5344
LID - 10.7150/jca.45394 [doi]
AB  - At present, no blood-based biomarkers have been used in clinical practice for 
      laryngeal squamous cell carcinoma (LSCC). Increasing evidence suggests that 
      circulating exosomal microRNAs (miRNAs) may serve as potential diagnostic 
      biomarkers for various cancers. This study aims to identify and evaluate serum 
      exosomal miRNAs for LSCC diagnosis. The ExoQuick solution (EQ), which provides a 
      high-yield and is a highly efficient exosome isolation method, was selected to 
      isolate serum exosomes in the current study. In LSCC samples, exosome 
      concentrations were higher than in healthy control (HC) samples. RNA-seq analysis 
      identified a total of 1608 miRNAs, with 34 upregulated and 41 downregulated in 
      LSCC samples relative to HC samples. Furthermore, qRT-PCR showed that miR-941 is 
      significantly upregulated in LSCC serum exosomes, with this same trend seen in 
      LSCC tissues and cells. Moreover, when examining miR-941 in cell lines, miR-941 
      overexpression promoted proliferation and invasion, while miR-941 knockdown 
      inhibited cell proliferation and invasion. ROC curve analysis showed that miR-941 
      has an area under the curve (AUC) of 0.797 (95% CI = 0.676-0.918) for 
      distinguishing LSCC patients from HCs. In conclusion, serum exosomal miR-941 may 
      serve as a promising oncogenic biomarker for diagnosing LSCC, and has the 
      potential as a therapeutic target.
CI  - (c) The author(s).
FAU - Zhao, Qinli
AU  - Zhao Q
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan 030001, Shanxi, P. R. China.
FAU - Zheng, Xiwang
AU  - Zheng X
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
FAU - Guo, Huina
AU  - Guo H
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
FAU - Xue, Xuting
AU  - Xue X
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
FAU - Zhang, Yuliang
AU  - Zhang Y
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
FAU - Niu, Min
AU  - Niu M
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
FAU - Cui, Jiajia
AU  - Cui J
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
FAU - Liu, Hongliang
AU  - Liu H
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
FAU - Luo, Hongjie
AU  - Luo H
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
FAU - Yang, Dongli
AU  - Yang D
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan 030001, Shanxi, P. R. China.
FAU - Shi, Yong
AU  - Shi Y
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan 030001, Shanxi, P. R. China.
FAU - Huangfu, Hui
AU  - Huangfu H
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
FAU - Wen, Shuxin
AU  - Wen S
AD  - Department of Otolaryngology Head & Neck Surgery, General Hospital of Shenzhen 
      University, Shenzhen 518061, Guangdong, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
FAU - Wu, Yongyan
AU  - Wu Y
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
AD  - Department of Biochemistry & Molecular Biology, Shanxi Medical University, 
      Taiyuan 030001, Shanxi, P. R. China.
AD  - Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical 
      University, Taiyuan, 030001 Shanxi, P. R. China.
FAU - Gao, Wei
AU  - Gao W
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
AD  - Department of Biochemistry & Molecular Biology, Shanxi Medical University, 
      Taiyuan 030001, Shanxi, P. R. China.
FAU - Wang, Binquan
AU  - Wang B
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan 030001, Shanxi, P. R. China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan 030001, 
      Shanxi, P. R. China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan 030001, Shanxi, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20200709
PL  - Australia
TA  - J Cancer
JT  - Journal of Cancer
JID - 101535920
PMC - PMC7391210
OTO - NOTNLM
OT  - biomarker
OT  - diagnosis
OT  - exosome
OT  - hsa-miR-941
OT  - laryngeal squamous cell carcinoma
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2020/08/04 06:00
MHDA- 2020/08/04 06:01
PMCR- 2020/01/01
CRDT- 2020/08/04 06:00
PHST- 2020/02/27 00:00 [received]
PHST- 2020/06/14 00:00 [accepted]
PHST- 2020/08/04 06:00 [entrez]
PHST- 2020/08/04 06:00 [pubmed]
PHST- 2020/08/04 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - jcav11p5329 [pii]
AID - 10.7150/jca.45394 [doi]
PST - epublish
SO  - J Cancer. 2020 Jul 9;11(18):5329-5344. doi: 10.7150/jca.45394. eCollection 2020.