PMID- 32742785
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220416
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 8
DP  - 2020
TI  - Identification of key genes of human bone marrow stromal cells adipogenesis at an 
      early stage.
PG  - e9484
LID - 10.7717/peerj.9484 [doi]
LID - e9484
AB  - BACKGROUND: Bone marrow adipocyte (BMA), closely associated with bone 
      degeneration, shares common progenitors with osteoblastic lineage. However, the 
      intrinsic mechanism of cells fate commitment between BMA and osteogenic lineage 
      remains unclear. METHODS: Gene Expression Omnibus (GEO) dataset GSE107789 
      publicly available was downloaded and analyzed. Differentially expressed genes 
      (DEGs) were analyzed using GEO2R. Functional and pathway enrichment analyses of 
      Gene Ontology and Kyoto Encyclopedia of Genes and Genomes were conducted by The 
      Database for Annotation, Visualization and Integrated Discovery and Gene set 
      enrichment analysis software. Protein-protein interactions (PPI) network was 
      obtained using STRING database, visualized and clustered by Cytoscape software. 
      Transcriptional levels of key genes were verified by real-time quantitative PCR 
      in vitro in Bone marrow stromal cells (BMSCs) undergoing adipogenic 
      differentiation at day 7 and in vivo in ovariectomized mice model. RESULTS: A 
      total of 2,869 DEGs, including 1,357 up-regulated and 1,512 down-regulated ones, 
      were screened out from transcriptional profile of human BMSCs undergoing 
      adipogenic induction at day 7 vs. day 0. Functional and pathway enrichment 
      analysis, combined with modules analysis of PPI network, highlighted ACSL1, 
      sphingosine 1-phosphate receptors 3 (S1PR3), ZBTB16 and glypican 3 as key genes 
      up-regulated at the early stage of BMSCs adipogenic differentiation. Furthermore, 
      up-regulated mRNA expression levels of ACSL1, S1PR3 and ZBTB16 were confirmed 
      both in vitro and in vivo. CONCLUSION: ACSL1, S1PR3 and ZBTB16 may play crucial 
      roles in early regulation of BMSCs adipogenic differentiation.
CI  - (c) 2020 Chen et al.
FAU - Chen, Pengyu
AU  - Chen P
AD  - Department of Orthopaedics, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong, China.
AD  - Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, 
      Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
FAU - Song, Mingrui
AU  - Song M
AD  - Department of Orthopaedics, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong, China.
AD  - Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, 
      Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
FAU - Wang, Yutian
AU  - Wang Y
AD  - Department of Orthopaedics, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong, China.
AD  - Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, 
      Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
FAU - Deng, Songyun
AU  - Deng S
AD  - Department of Orthopaedics, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong, China.
AD  - Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, 
      Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
FAU - Hong, Weisheng
AU  - Hong W
AD  - Department of Orthopaedics, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong, China.
AD  - Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, 
      Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
FAU - Zhang, Xianrong
AU  - Zhang X
AUID- ORCID: 0000-0002-0992-5013
AD  - Department of Orthopaedics, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong, China.
AD  - Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, 
      Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
FAU - Yu, Bin
AU  - Yu B
AD  - Department of Orthopaedics, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong, China.
AD  - Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, 
      Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
LA  - eng
PT  - Journal Article
DEP - 20200721
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC7380279
OTO - NOTNLM
OT  - Adipogenesis
OT  - Bioinformatics
OT  - Bone marrow adipocytes
OT  - Bone marrow stromal cells
OT  - Differentially expressed genes
COIS- The authors declare that they have no competing interests.
EDAT- 2020/08/04 06:00
MHDA- 2020/08/04 06:01
PMCR- 2020/07/21
CRDT- 2020/08/04 06:00
PHST- 2020/03/20 00:00 [received]
PHST- 2020/06/15 00:00 [accepted]
PHST- 2020/08/04 06:00 [entrez]
PHST- 2020/08/04 06:00 [pubmed]
PHST- 2020/08/04 06:01 [medline]
PHST- 2020/07/21 00:00 [pmc-release]
AID - 9484 [pii]
AID - 10.7717/peerj.9484 [doi]
PST - epublish
SO  - PeerJ. 2020 Jul 21;8:e9484. doi: 10.7717/peerj.9484. eCollection 2020.