PMID- 32743127
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210923
IS  - 2468-2330 (Electronic)
IS  - 2468-2330 (Linking)
VI  - 1
DP  - 2018 Mar
TI  - Mitogen activated protein kinases (MAPK) and protein phosphatases are involved in 
      Aspergillus fumigatus adhesion and biofilm formation.
PG  - 43-56
LID - 10.1016/j.tcsw.2018.03.002 [doi]
AB  - The main characteristic of biofilm formation is extracellular matrix (ECM) 
      production. The cells within the biofilm are surrounded by ECM which provides 
      structural integrity and protection. During an infection, this protection is 
      mainly against cells of the immune system and antifungal drugs. A. fumigatus 
      forms biofilms during static growth on a solid substratum and in chronic 
      aspergillosis infections. It is important to understand how, and which, A. 
      fumigatus signal transduction pathways are important for the adhesion and biofilm 
      formation in a host during infection. Here we investigated the role of MAP 
      kinases and protein phosphatases in biofilm formation. The loss of the MAP 
      kinases MpkA, MpkC and SakA had an impact on the cell surface and the ECM during 
      biofilm formation and reduced the adherence of A. fumigatus to polystyrene and 
      fibronectin-coated plates. The phosphatase null mutants DeltasitA and DeltaptcB, involved 
      in regulation of MpkA and SakA phosphorylation, influenced cell wall carbohydrate 
      exposure. Moreover, we characterized the A. fumigatus protein phosphatase PphA. 
      The DeltapphA strain was more sensitive to cell wall-damaging agents, had increased 
      beta-(1,3)-glucan and reduced chitin, decreased conidia phagocytosis by 
      Dictyostelium discoideum and reduced adhesion and biofilm formation. Finally, 
      DeltapphA strain was avirulent in a murine model of invasive pulmonary aspergillosis 
      and increased the released of tumor necrosis factor alpha (TNF-alpha) from bone 
      marrow derived macrophages (BMDMs). These results show that MAP kinases and 
      phosphatases play an important role in signaling pathways that regulate the 
      composition of the cell wall, extracellular matrix production as well as adhesion 
      and biofilm formation in A. fumigatus.
CI  - (c) 2018 Elsevier B.V.
FAU - Manfiolli, Adriana Oliveira
AU  - Manfiolli AO
AD  - Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, 
      Ribeirao Preto, Brazil.
FAU - Dos Reis, Thaila Fernanda
AU  - Dos Reis TF
AD  - Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, 
      Ribeirao Preto, Brazil.
FAU - de Assis, Leandro Jose
AU  - de Assis LJ
AD  - Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, 
      Ribeirao Preto, Brazil.
FAU - de Castro, Patricia Alves
AU  - de Castro PA
AD  - Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, 
      Ribeirao Preto, Brazil.
FAU - Silva, Lilian Pereira
AU  - Silva LP
AD  - Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, 
      Ribeirao Preto, Brazil.
FAU - Hori, Juliana I
AU  - Hori JI
AD  - Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, 
      Ribeirao Preto, Brazil.
FAU - Walker, Louise A
AU  - Walker LA
AD  - School of Medical Sciences, University of Aberdeen, Aberdeen, UK.
FAU - Munro, Carol A
AU  - Munro CA
AD  - School of Medical Sciences, University of Aberdeen, Aberdeen, UK.
FAU - Rajendran, Ranjith
AU  - Rajendran R
AD  - Infection and Immunity Research Group, Glasgow Dental School, School of Medicine, 
      College of Medical, Veterinary and Life Sciences, The University of Glasgow, 378 
      Sauchiehall Street, Glasgow G2 3JZ, UK.
FAU - Ramage, Gordon
AU  - Ramage G
AD  - Infection and Immunity Research Group, Glasgow Dental School, School of Medicine, 
      College of Medical, Veterinary and Life Sciences, The University of Glasgow, 378 
      Sauchiehall Street, Glasgow G2 3JZ, UK.
FAU - Goldman, Gustavo H
AU  - Goldman GH
AD  - Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, 
      Ribeirao Preto, Brazil.
LA  - eng
GR  - MR/N006364/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
DEP - 20180326
PL  - Netherlands
TA  - Cell Surf
JT  - Cell surface (Amsterdam, Netherlands)
JID - 101728565
EIN - Cell Surf. 2020 Jan 23;6:100035. PMID: 32744537
PMC - PMC7389341
OTO - NOTNLM
OT  - Aspergillus fumigatus
OT  - Biofilm
OT  - Extracellular matrix
OT  - Mitogen activated protein kinases
OT  - Phosphatases
OT  - Polysaccharide
EDAT- 2018/03/26 00:00
MHDA- 2018/03/26 00:01
PMCR- 2018/03/26
CRDT- 2020/08/04 06:00
PHST- 2018/02/09 00:00 [received]
PHST- 2018/03/08 00:00 [revised]
PHST- 2018/03/14 00:00 [accepted]
PHST- 2020/08/04 06:00 [entrez]
PHST- 2018/03/26 00:00 [pubmed]
PHST- 2018/03/26 00:01 [medline]
PHST- 2018/03/26 00:00 [pmc-release]
AID - S2468-2330(18)30004-5 [pii]
AID - 10.1016/j.tcsw.2018.03.002 [doi]
PST - epublish
SO  - Cell Surf. 2018 Mar 26;1:43-56. doi: 10.1016/j.tcsw.2018.03.002. eCollection 2018 
      Mar.