PMID- 32744004
OWN - NLM
STAT- MEDLINE
DCOM- 20201118
LR  - 20201118
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 36
IP  - 2
DP  - 2020 Mar
TI  - [Effects of mice macrophages on skeletal muscle cells under high glucose 
      treatment].
PG  - 124-129
LID - 10.12047/j.cjap.5901.2020.028 [doi]
AB  - Objective: To study the effects of mice macrophages on myogenic differentiation 
      and insulin sensitivity of skeletal muscle cells under high glucose condition. 
      Methods: C2C12 myoblasts and RAW264. 7 macrophages were co-cultured in transwell 
      and treated with 60 mmol/L glucose. They were randomly divided into single 
      culture control group (SC group, n=12), co-culture control group (CC group, 
      n=12), single culture high glucose group (SH group, n=12) and co-culture high 
      glucose group (CH group, n=12). Cell morphology was observed by phase contrast 
      microscope. C2C12 were collected after 1 and 3 days of co-culture. Cell viability 
      was measured by CCK-8. Embryonic myosin heavy chain (E-MHC) and glucose 
      transporters 4 (GLUT4) protein expressions were detected by immunofluorescence. 
      The expressions of myogenic factor 5 (Myf5), myogenic determination gene (MyoD) 
      and myogenin gene were detected by real-time PCR. 
      2-(N-(7-nitrobenz-2-oxa-13-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) assay was 
      used to detect the cellular basis and insulin-stimulated glucose uptake. Results: 
      Under normal glucose concentration, this co-culture with RAW264. 7 promoted C2C12 
      myotube formation, E-MHC protein expression (P＜0. 01), MyoD and myogenin gene 
      expressions (P＜ 0. 05), insulin-stimulated 2-NBDG uptake (P＜0. 05), and basic 
      GLUT4 level (P＜0. 05). High glucose stimulation inhibited myotube formation, 
      myogenic regulatory factor gene expression, 2-NBDG uptake and GLUT4 expression in 
      C2C12 (P＜0. 05). When co-cultured with C2C12 under high glucose treatment, 
      compared with co-culture control group and high glucose group, cell activity, 
      E-MHC protein expression, myogenic regulator gene expressions, 2-NBDG uptake and 
      GLUT4 protein expression were significantly decreased (P＜0. 05). Conclusion: 
      Co-culture with RAW264. 7 promotes myogenic differentiation and increases insulin 
      sensitivity in C2C12, but this effect is reversed under 60 mmol/L glucose 
      treatment, which inhibits myogenic differentiation and induces insulin 
      resistance.
FAU - Luo, Wei
AU  - Luo W
AD  - Department of Sports and Health Sciences, Nanjing Sport Institute, Nanjing 
      210014.
AD  - Department of Exercise Physiology, Beijing Sport University, Beijing 100084.
FAU - Ai, Lei
AU  - Ai L
AD  - Jiangsu Research Institute of Sports Science, Nanjing 210033, China.
FAU - Wang, Bo-Fa
AU  - Wang BF
AD  - Department of Exercise Physiology, Beijing Sport University, Beijing 100084.
FAU - Wang, Li-Ying
AU  - Wang LY
AD  - Department of Exercise Physiology, Beijing Sport University, Beijing 100084.
FAU - Gan, Yan-Ming
AU  - Gan YM
AD  - Department of Exercise Physiology, Beijing Sport University, Beijing 100084.
FAU - Zhou, Yue
AU  - Zhou Y
AD  - Department of Exercise Physiology, Beijing Sport University, Beijing 100084.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (GLUT4 enhancer factor, mouse)
RN  - 0 (Myogenin)
RN  - 0 (Transcription Factors)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - DNA-Binding Proteins/metabolism
MH  - Glucose
MH  - Macrophages/*cytology
MH  - Mice
MH  - Muscle Fibers, Skeletal
MH  - Myoblasts/*cytology
MH  - Myogenin/genetics
MH  - Myosin Heavy Chains/metabolism
MH  - RAW 264.7 Cells
MH  - Transcription Factors/metabolism
OTO - NOTNLM
OT  - co-culture
OT  - high glucose
OT  - insulin sensitivity
OT  - mice
OT  - myocyte
OT  - myogenic differentiation
EDAT- 2020/08/04 06:00
MHDA- 2020/11/20 06:00
CRDT- 2020/08/04 06:00
PHST- 2020/08/04 06:00 [entrez]
PHST- 2020/08/04 06:00 [pubmed]
PHST- 2020/11/20 06:00 [medline]
AID - 10.12047/j.cjap.5901.2020.028 [doi]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2020 Mar;36(2):124-129. doi: 
      10.12047/j.cjap.5901.2020.028.