PMID- 32751764
OWN - NLM
STAT- MEDLINE
DCOM- 20210217
LR  - 20210217
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 10
IP  - 8
DP  - 2020 Jul 30
TI  - Effect of Methionine Diet on Time-Related Metabolic and Histopathological Changes 
      of Rat Hippocampus in the Model of Global Brain Ischemia.
LID - 10.3390/biom10081128 [doi]
LID - 1128
AB  - Hyperhomocysteinemia (hHcy) represents a strong risk factor for 
      atherosclerosis-associated diseases, like stroke, dementia or Alzheimer's 
      disease. A methionine (Met)-rich diet leads to an elevated level of homocysteine 
      in plasma and might cause pathological alterations across the brain. The 
      hippocampus is being constantly studied for its selective vulnerability linked 
      with neurodegeneration. This study explores metabolic and histo-morphological 
      changes in the rat hippocampus after global ischemia in the hHcy conditions using 
      a combination of proton magnetic resonance spectroscopy and magnetic 
      resonance-volumetry as well as immunohistochemical analysis. After 4 weeks of a 
      Met-enriched diet at a dose of 2 g/kg of animal weight/day, adult male Wistar 
      rats underwent 4-vessel occlusion lasting for 15 min, followed by a reperfusion 
      period varying from 3 to 7 days. Histo-morphological analyses showed that the 
      subsequent ischemia-reperfusion insult (IRI) aggravates the extent of the sole 
      hHcy-induced degeneration of the hippocampal neurons. Decreased volume in the 
      grey matter, extensive changes in the metabolic ratio, deeper alterations in the 
      number and morphology of neurons, astrocytes and their processes were 
      demonstrated in the hippocampus 7 days post-ischemia in the hHcy animals. Our 
      results suggest that the combination of the two risk factors (hHcy and IRI) 
      endorses and exacerbates the rat hippocampal neurodegenerative processes.
FAU - Kovalska, Maria
AU  - Kovalska M
AD  - Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
FAU - Hnilicova, Petra
AU  - Hnilicova P
AUID- ORCID: 0000-0003-1529-1675
AD  - Department of Neuroscience, Biomedical Center Martin, Jessenius Faculty of 
      Medicine, Comenius University in Bratislava, 03601 Martin, Slovakia.
FAU - Kalenska, Dagmar
AU  - Kalenska D
AD  - Department of Anatomy, Jessenius Faculty of Medicine, Comenius University in 
      Bratislava, 03601 Martin, Slovakia.
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
FAU - Tomascova, Anna
AU  - Tomascova A
AD  - Department of Neuroscience, Biomedical Center Martin, Jessenius Faculty of 
      Medicine, Comenius University in Bratislava, 03601 Martin, Slovakia.
FAU - Adamkov, Marian
AU  - Adamkov M
AD  - Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
FAU - Lehotsky, Jan
AU  - Lehotsky J
AD  - Department of Neuroscience, Biomedical Center Martin, Jessenius Faculty of 
      Medicine, Comenius University in Bratislava, 03601 Martin, Slovakia.
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
LA  - eng
GR  - VEGA 1/0171/18/Agentura na Podporu Vyskumu a Vyvoja/International
GR  - VEGA 1/0230/20/Vedecka Grantova Agentura MSVVaS SR a SAV/International
GR  - APVV 15/0107/Ministry of Education of the Slovak Republic/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200730
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/complications/*metabolism/pathology
MH  - *Diet
MH  - Hippocampus/diagnostic imaging/drug effects/*metabolism/pathology
MH  - Homocysteine/metabolism
MH  - Hyperhomocysteinemia/*complications/etiology
MH  - Magnetic Resonance Spectroscopy
MH  - Male
MH  - Methionine/administration & dosage/*adverse effects
MH  - Rats
MH  - Rats, Wistar
PMC - PMC7465067
OTO - NOTNLM
OT  - 1H MRS
OT  - brain ischemia and reperfusion
OT  - hippocampus
OT  - methionine diet
OT  - neurodegeneration
OT  - rat
COIS- The authors declare no conflict of interest.
EDAT- 2020/08/06 06:00
MHDA- 2021/02/18 06:00
PMCR- 2020/08/01
CRDT- 2020/08/06 06:00
PHST- 2020/06/29 00:00 [received]
PHST- 2020/07/24 00:00 [revised]
PHST- 2020/07/29 00:00 [accepted]
PHST- 2020/08/06 06:00 [entrez]
PHST- 2020/08/06 06:00 [pubmed]
PHST- 2021/02/18 06:00 [medline]
PHST- 2020/08/01 00:00 [pmc-release]
AID - biom10081128 [pii]
AID - biomolecules-10-01128 [pii]
AID - 10.3390/biom10081128 [doi]
PST - epublish
SO  - Biomolecules. 2020 Jul 30;10(8):1128. doi: 10.3390/biom10081128.