PMID- 32753906
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220416
IS  - 1178-6973 (Print)
IS  - 1178-6973 (Electronic)
IS  - 1178-6973 (Linking)
VI  - 13
DP  - 2020
TI  - The Osteogenic Effect of Local Delivery of Vancomycin and Tobramycin on Bone 
      Marrow Stromal Cells.
PG  - 2083-2091
LID - 10.2147/IDR.S261767 [doi]
AB  - PURPOSE: Bone tissue infections are a difficult problem in orthopedic surgery. 
      Topical application of vancomycin and tobramycin powder has been proved to 
      significantly reduce infection rates. However, the osteogenic effect of the 
      topical application of these two antibiotics is unclear. In this study, the 
      osteogenic effect of local delivery antibiotics on bone regeneration was 
      investigated in vitro. METHODS: Bone marrow stromal cells (BMSCs) were incubated 
      in the presence of vancomycin (14.28mug/mL), tobramycin (28.57mug/mL), or 
      vancomycin combined with tobramycin (vancomycin 14.28mug/mL and tobramycin 
      28.57mug/mL). Cell viability, proliferation, and migration were analyzed. The 
      alizarin red staining as well as the alkaline phosphatase staining was 
      investigated. Then, the quantitative real-time (qRT)-PCR of osteogenic mRNA 
      expression levels were also evaluated. RESULTS: The results showed that 
      vancomycin combined with tobramycin has no adverse effect on the viability and 
      proliferation of BMSCs. The topical application of vancomycin alone may interfere 
      with the bone regenerative processes. However, the tobramycin can promote the 
      osteogenic differentiation of BMSCs and also rescue the osteogenic potential of 
      BMSCs inhibited by vancomycin both in vitro. CONCLUSION: From this in vitro 
      study, local application of vancomycin combined with tobramycin does not affect 
      the osteogenic potential of BMSCs.
CI  - (c) 2020 Yu et al.
FAU - Yu, Lingjia
AU  - Yu L
AD  - Department of Orthopedics, Beijing Friendship Hospital, Capital Medical 
      University, Beijing 100050, People's Republic of China.
FAU - Fei, Qi
AU  - Fei Q
AUID- ORCID: 0000-0001-9753-2692
AD  - Department of Orthopedics, Beijing Friendship Hospital, Capital Medical 
      University, Beijing 100050, People's Republic of China.
FAU - Lin, Jisheng
AU  - Lin J
AD  - Department of Orthopedics, Beijing Friendship Hospital, Capital Medical 
      University, Beijing 100050, People's Republic of China.
FAU - Yang, Yong
AU  - Yang Y
AD  - Department of Orthopedics, Beijing Friendship Hospital, Capital Medical 
      University, Beijing 100050, People's Republic of China.
FAU - Xu, Yisheng
AU  - Xu Y
AD  - Orthopedics Department, Guangdong Provincial Hospital of Traditional Chinese 
      Medicine, Guangzhou, Guangdong 510120, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20200701
PL  - New Zealand
TA  - Infect Drug Resist
JT  - Infection and drug resistance
JID - 101550216
PMC - PMC7351982
OTO - NOTNLM
OT  - bone regeneration
OT  - osteogenesis
OT  - tobramycin
OT  - vancomycin
COIS- The authors report no conflicts of interest in this work.
EDAT- 2020/08/06 06:00
MHDA- 2020/08/06 06:01
PMCR- 2020/07/01
CRDT- 2020/08/06 06:00
PHST- 2020/05/13 00:00 [received]
PHST- 2020/06/18 00:00 [accepted]
PHST- 2020/08/06 06:00 [entrez]
PHST- 2020/08/06 06:00 [pubmed]
PHST- 2020/08/06 06:01 [medline]
PHST- 2020/07/01 00:00 [pmc-release]
AID - 261767 [pii]
AID - 10.2147/IDR.S261767 [doi]
PST - epublish
SO  - Infect Drug Resist. 2020 Jul 1;13:2083-2091. doi: 10.2147/IDR.S261767. 
      eCollection 2020.