PMID- 32754136
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 11
DP  - 2020
TI  - Polymicrobial Biofilm Interaction Between Histophilus somni and Pasteurella 
      multocida.
PG  - 1561
LID - 10.3389/fmicb.2020.01561 [doi]
LID - 1561
AB  - Histophilus somni and Pasteurella multocida are two of multiple agents 
      responsible for bovine respiratory disease (BRD) in cattle. Following respiratory 
      infection of calves with H. somni, P. multocida may also be isolated from the 
      lower respiratory tract. Because H. somni may form a biofilm during BRD, we 
      sought to determine if P. multocida can co-exist with H. somni in a polymicrobial 
      biofilm in vitro and in vivo. Interactions between the two species in the biofilm 
      were characterized and quantified by fluorescence in situ hybridization (FISH). 
      The biofilm matrix of each species was examined using fluorescently tagged 
      lectins (FTL) specific for the exopolysaccharide (EPS) using confocal laser 
      scanning microscopy. Bacterial interactions were determined by auto-aggregation 
      and biofilm morphology. Pasteurella multocida and H. somni were evenly 
      distributed in the in vitro biofilm, and both species contributed to the 
      polymicrobial biofilm matrix. The average biomass and biofilm thickness, and the 
      total carbohydrate and protein content of the biofilm, were greatest when both 
      species were present. Polymicrobial bacterial suspensions auto-aggregated faster 
      than single species suspensions, suggesting physical interactions between the two 
      species. Almost 300 P. multocida genes were significantly differentially 
      regulated when the bacteria were in a polymicrobial biofilm compared to a 
      mono-species biofilm, as determined by RNA-sequencing. As expected, host genes 
      associated with inflammation and immune response were significantly upregulated 
      at the infection site following H. somni challenge. Encapsulated P. multocida 
      isolates not capable of forming a substantial biofilm enhanced an in vitro 
      polymicrobial biofilm with H. somni, indicating they contributed to the 
      polymicrobial biofilm matrix. Indirect evidence indicated that encapsulated P. 
      multocida also contributed to a polymicrobial biofilm in vivo. Only the EPS of H. 
      somni could be detected by FTL staining of bovine tissues following challenge 
      with H. somni. However, both species were isolated and an immune response to the 
      biofilm matrix of both species was greater than the response to planktonic cells, 
      suggesting encapsulated P. multocida may take advantage of the H. somni biofilm 
      to persist in the host during chronic BRD. These results may have important 
      implications for the management and prevention of BRD.
CI  - Copyright (c) 2020 Petruzzi, Dickerman, Lahmers, Scarratt and Inzana.
FAU - Petruzzi, Briana
AU  - Petruzzi B
AD  - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Tech, Blacksburg, VA, United States.
FAU - Dickerman, Allan
AU  - Dickerman A
AD  - Biocomplexity Institute and Initiative, University of Virginia, Virginia Tech, 
      Charlottesville, VA, United States.
FAU - Lahmers, Kevin
AU  - Lahmers K
AD  - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Tech, Blacksburg, VA, United States.
FAU - Scarratt, William K
AU  - Scarratt WK
AD  - Department of Large Animal Clinical Sciences, Virginia-Maryland College of 
      Veterinary Medicine, Blacksburg, VA, United States.
FAU - Inzana, Thomas J
AU  - Inzana TJ
AD  - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Tech, Blacksburg, VA, United States.
AD  - Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, 
      Long Island University, Brookville, NY, United States.
LA  - eng
PT  - Journal Article
DEP - 20200710
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC7366659
OTO - NOTNLM
OT  - Histophilus somni
OT  - Pasteurella multocida
OT  - biofilm
OT  - bovine respiratory disease
OT  - polymicrobial
EDAT- 2020/08/06 06:00
MHDA- 2020/08/06 06:01
PMCR- 2020/07/10
CRDT- 2020/08/06 06:00
PHST- 2020/04/27 00:00 [received]
PHST- 2020/06/16 00:00 [accepted]
PHST- 2020/08/06 06:00 [entrez]
PHST- 2020/08/06 06:00 [pubmed]
PHST- 2020/08/06 06:01 [medline]
PHST- 2020/07/10 00:00 [pmc-release]
AID - 10.3389/fmicb.2020.01561 [doi]
PST - epublish
SO  - Front Microbiol. 2020 Jul 10;11:1561. doi: 10.3389/fmicb.2020.01561. eCollection 
      2020.