PMID- 32755629
OWN - NLM
STAT- MEDLINE
DCOM- 20210430
LR  - 20210430
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 736
DP  - 2020 Sep 25
TI  - NMDARs contribute to the facial stimuli-evoked mossy fiber-granule cell synaptic 
      transmission in vivo in mice.
PG  - 135285
LID - S0304-3940(20)30555-3 [pii]
LID - 10.1016/j.neulet.2020.135285 [doi]
AB  - N-methyl-D-aspartate receptors (NMDARs) are expressed in granule cell and involve 
      in mossy fiber-granule cell (MF-GC) synaptic transmission in cerebellar cortex. 
      In the absence GABA(A) receptor activity, we here studied the role of NMDARs 
      during the facial stimulation evoked MF-GC synaptic transmission in 
      urethane-anesthetized mice using electrophysiological recording technique and 
      pharmacological methods. Our results showed that facial stimuli train (20 Hz, 5 
      pulses) evoked 5 field potential responses (N1-N5) in mouse cerebellar granular 
      layer, which identified MF-GC synaptic transmission. Blocking NMDARs induced 
      significant depression in the amplitude of N2 to N5, accompanied with significant 
      decrease in pulse ratios, area under the curve (AUC) and half-width of N1. A 
      selective GluN2A antagonist, PEAQX (10 muM) also produced significant depression 
      in the amplitude of N2 to N5, and decreases in pulse ratios. However, a selective 
      GluN2B antagonist, TCN-237 (10 muM) did not significantly attenuate the facial 
      stimuli train-induced mossy fiber-granule cell synaptic transmission. Application 
      of NMDA (1 muM) produced increases in the AUC and half-width of R(on), as well the 
      amplitude and AUC of R(off), which was reversed by following application of 
      PEAQX. Our present results indicated that NMDARs, especially GluN2A contribute to 
      the facial stimulation-evoked MF-GC synaptic transmission, suggesting that the 
      NMDARs play an important role during the lateral sensory information synaptic 
      transmission in the cerebellar granular layer in vivo in mice.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Zhang, Xin-Yuan
AU  - Zhang XY
AD  - Brain Science Research Center, Yanbian University, Yanji, China; Department of 
      Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, 
      China.
FAU - Zhang, Guang-Jian
AU  - Zhang GJ
AD  - Department of Pain, Affiliated Hospital of Yanbian University, Yanji, Jilin 
      Province, China.
FAU - Li, Bing-Xue
AU  - Li BX
AD  - Brain Science Research Center, Yanbian University, Yanji, China; Department of 
      Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, 
      China.
FAU - Bing, Yan-Hua
AU  - Bing YH
AD  - Brain Science Research Center, Yanbian University, Yanji, China.
FAU - Cui, Bai-Ri
AU  - Cui BR
AD  - Department of Osteology, Affiliated Hospital of Yanbian University, Yanji, Jilin 
      Province, China.
FAU - Cui, Li-Na
AU  - Cui LN
AD  - Department of Acupuncture, Affiliated Hospital of Yanbian University, Yanji, 
      Jilin Province, China.
FAU - Chu, Chun-Ping
AU  - Chu CP
AD  - Brain Science Research Center, Yanbian University, Yanji, China; Department of 
      Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, 
      China.
FAU - Qiu, De-Lai
AU  - Qiu DL
AD  - Brain Science Research Center, Yanbian University, Yanji, China; Department of 
      Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, 
      China. Electronic address: dlqiu@ybu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200802
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 
      (5-(alpha-methyl-4-bromobenzylamino)phosphonomethyl-1,4-dihydroquinoxaline-2,3-dione)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Quinoxalines)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 76326-31-3 (2-amino-5-phosphopentanoic acid)
RN  - HG18B9YRS7 (Valine)
SB  - IM
MH  - Animals
MH  - Cerebellar Cortex/drug effects/*physiology
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Mice
MH  - Mice, Inbred ICR
MH  - N-Methylaspartate/pharmacology
MH  - Neurons/drug effects/*physiology
MH  - Physical Stimulation
MH  - Quinoxalines/pharmacology
MH  - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors/*physiology
MH  - Synaptic Transmission/drug effects/*physiology
MH  - Valine/analogs & derivatives/pharmacology
MH  - Vibrissae/physiology
OTO - NOTNLM
OT  - In vivo electrophysiological recording
OT  - Mossy fiber-granule cell synaptic transmission
OT  - N-methyl-d-aspartate receptors (NMDARs)
OT  - Neuropharmacology
OT  - Sensory stimulation
EDAT- 2020/08/07 06:00
MHDA- 2021/05/01 06:00
CRDT- 2020/08/07 06:00
PHST- 2020/06/26 00:00 [received]
PHST- 2020/07/27 00:00 [revised]
PHST- 2020/07/29 00:00 [accepted]
PHST- 2020/08/07 06:00 [pubmed]
PHST- 2021/05/01 06:00 [medline]
PHST- 2020/08/07 06:00 [entrez]
AID - S0304-3940(20)30555-3 [pii]
AID - 10.1016/j.neulet.2020.135285 [doi]
PST - ppublish
SO  - Neurosci Lett. 2020 Sep 25;736:135285. doi: 10.1016/j.neulet.2020.135285. Epub 
      2020 Aug 2.