PMID- 32755652 OWN - NLM STAT- MEDLINE DCOM- 20210225 LR - 20210225 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 263 DP - 2020 Dec 5 TI - Sojadodamgangki-tang attenuates allergic lung inflammation by inhibiting T helper 2 cells and Augmenting alveolar macrophages. PG - 113152 LID - S0378-8741(20)33034-8 [pii] LID - 10.1016/j.jep.2020.113152 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Sojadodamgangki-tang (SDG) is a traditional East-Asian herbal medicine mainly composed of Pinellia ternate (Thunb.) Makino, Perilla frutescens (L.) Britt and 10 kinds of medicinal herbs. It has been used to treat asthma and mucus secretion including lung and bronchi. AIM OF THE STUDY: The aim of this study was to investigate the anti-inflammatory effects of Sojadodamgangki-tang (SDG) on allergic lung inflammation in vitro and in vivo as well as the underlying mechanisms. MATERIALS AND METHODS: We used an ovalbumin (OVA)-induced murine allergic airway inflammation model. Five groups of 8-week-old female BALB/C mice were divided into the following groups: saline control group, the vehicle (allergic) group that received OVA only, groups that received OVA and SDG (200 mg/kg or 400 mg/kg), and a positive control group that received OVA and Dexamethasone (5 mg/kg). In vitro experiments include T helper 2 (TH2) polarization system, murine macrophage cell culture, and human bronchial epithelial cell line (BEAS-2B) culture. RESULTS: SDG administration reduced allergic airway inflammatory cell infiltration, especially of eosinophils, mucus production, Th2 cell activation, OVA-specific immunoglobulin E (IgE), and total IgE production. Moreover, the activation of alveolar macrophages, which leads to immune tolerance in the steady state, was promoted by SDG treatment. Interestingly, SDG treatment also reduced the production of alarmin cytokines by the human bronchial epithelial cell line BEAS-2B stimulated with urban particulate matter. CONCLUSION: Our findings indicate that SDG has potential as a therapeutic drug to inhibit Th2 cell activation and promote alveolar macrophage activation. CI - Copyright (c) 2020 Elsevier B.V. All rights reserved. FAU - Jung, Seyoung AU - Jung S AD - Department of Pathology, College of Korean Medicine, Sangji University, Wonju-si, Gangwon-do, 26339, Republic of Korea. Electronic address: jxparao@naver.com. FAU - Park, Junkyu AU - Park J AD - Department of Pathology, College of Korean Medicine, Sangji University, Wonju-si, Gangwon-do, 26339, Republic of Korea. Electronic address: wnsrb1995@naver.com. FAU - Park, Jiwon AU - Park J AD - Department of Pathology, College of Korean Medicine, Sangji University, Wonju-si, Gangwon-do, 26339, Republic of Korea; Kyunghee University Medical Center, Kyunghee University, Seoul, 02447, Republic of Korea. Electronic address: wldnjdjs@naver.com. FAU - Jo, Hanna AU - Jo H AD - Department of Pathology, College of Korean Medicine, Sangji University, Wonju-si, Gangwon-do, 26339, Republic of Korea. Electronic address: dorejoah@naver.com. FAU - Seo, Chang-Seob AU - Seo CS AD - Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea. Electronic address: csseo0914@kiom.re.kr. FAU - Jeon, Woo-Young AU - Jeon WY AD - Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea. Electronic address: ssamggun85@kiom.re.kr. FAU - Lee, Mee-Young AU - Lee MY AD - Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea. Electronic address: cozy11@kiom.re.kr. FAU - Kwon, Bo-In AU - Kwon BI AD - Department of Pathology, College of Korean Medicine, Sangji University, Wonju-si, Gangwon-do, 26339, Republic of Korea; Research Institute of Korean Medicine, Sangji University, Wonju-si, Gangwon-do, 26339, Republic of Korea. Electronic address: kbi34812@sangji.ac.kr. LA - eng PT - Journal Article DEP - 20200802 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Anti-Asthmatic Agents) RN - 0 (Drugs, Chinese Herbal) RN - 9006-59-1 (Ovalbumin) SB - IM MH - Animals MH - Anti-Asthmatic Agents/isolation & purification/pharmacology/*therapeutic use MH - Asthma/chemically induced/*drug therapy/metabolism MH - Dose-Response Relationship, Drug MH - Drugs, Chinese Herbal/isolation & purification/pharmacology/*therapeutic use MH - Female MH - Macrophages, Alveolar/*drug effects/metabolism MH - Mice MH - Mice, Inbred BALB C MH - Ovalbumin/toxicity MH - Perilla MH - Pinellia MH - Th2 Cells/*drug effects/metabolism OTO - NOTNLM OT - Alarmin OT - Allergic lung inflammation OT - Alveolar macrophage OT - Bronchoalveolar lavage fluid OT - Particulate matter OT - Sojadodamgangki-tang OT - Th2 cells activation EDAT- 2020/08/07 06:00 MHDA- 2021/02/26 06:00 CRDT- 2020/08/07 06:00 PHST- 2020/03/09 00:00 [received] PHST- 2020/07/01 00:00 [revised] PHST- 2020/07/02 00:00 [accepted] PHST- 2020/08/07 06:00 [pubmed] PHST- 2021/02/26 06:00 [medline] PHST- 2020/08/07 06:00 [entrez] AID - S0378-8741(20)33034-8 [pii] AID - 10.1016/j.jep.2020.113152 [doi] PST - ppublish SO - J Ethnopharmacol. 2020 Dec 5;263:113152. doi: 10.1016/j.jep.2020.113152. Epub 2020 Aug 2.